Clinical significance and intestinal microbiota composition in immunocompromised children with norovirus gastroenteritis

Abstract

Background Norovirus (NoV) infection is common in pediatric patients with immunodeficiency and is more likely to cause severe disease. Objective Our study aims to figure out the clinical differences and distribution of intestinal microbiota in immunocompromised children with NoV gastroenteritis. Methods Pediatric patients admitted to Shang-Ho Hospital with diagnosis of acute gastroenteritis including different immune status were enrolled and their medical records were reviewed. NoV gastroenteritis was validated using RT-PCR molecular methods. Viral shedding period was determined by real-time RT-PCR assays. Intestinal microbiota enrichment analysis was carried out by next generation sequencing after fecal DNA extraction and subsequent Linear Discriminant Analysis (LDA) Effect Size (LEfSe) method. Results Significantly higher frequency of diarrhea [mean, (IQR), 3.8 (3–5) /day] and longer viral shedding time [mean, IQR, 8.5 (5–13) days] was found in immunocompromised NoV infections than in immunocompetent patients without NoV infections (p = 0.013*) and immunocompetent patients with NoV infections (p = 0.030**). The fever prevalence was significantly lower in immunocompromised NoV infections than in different immune or infection status. Intestinal microbiota metagenomics analysis showed no significant community richness difference while the LEfSe analysis showed a significant difference in commensal richness at the phylum level, the family level, and the genus level in patients under different immune status. Conclusion We evaluated the clinical significances and microbiota composition in immunocompromised children with norovirus gastroenteritis. This will further facilitate studies of the interaction between the intestinal microbiota in such patients with precise determination of their bacterial infection control and probiotic supplements strategy.

Prolonged viral shedding in feces of children with COVID-19: a systematic review and synthesis of data

“…A study of 104 COVID-19 patients by Hao et al reported that a decrease in T cells and B cells was associated with prolonged viral RNA shedding [ 38 ]. For other respiratory viral infections, such as influenza [ 39 ], adenovirus [ 40 ], and norovirus [ 41 ], current data available suggest high rates of asymptomatic carriage in the stool and a prolonged carrier state in children, which are related in part to children’s current stage of immune development. It can be interpreted that prolonged fecal viral RNA shedding in children with COVID-19 may be related to the immaturity of their immune systems.…”

“…Third, gut microbiota has been reported to play a key role in determining the sensitivity of patients to viral infection. An animal study showed that the bacterial microbiome prevented persistent murine norovirus infection via the replenishment of the bacterial microbiota related to host immune specificity [ 41 ]. Another research has confirmed that Coprobacillus spp.…”