Clinical Presentation Resembling Mucosal Disease Associated with ‘HoBi’-like Pestivirus in a Field Outbreak

Weber, Matheus Nunes; Mósena, Ana Cristina Sbaraini; Simões, Sara Vilar Dantas; Almeida, Laura Lopes de; Pessoa, Clarice R.M.; Budaszewski, R.F.; Silva, T. R.; Ridpath, Julia F.; Riet-Correa, Franklin; Driemeier, David; Canal, Cláudio Wageck

doi:10.1111/tbed.12223

Cited by 54 publications

(86 citation statements)

References 41 publications

(81 reference statements)

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“…A árvore filogenética obtida (Fig.2) demonstra seis clusters separados que correspondem aos pestivirus BVDV-1, BVDV-2, CSFV, BDV, vírus Pronghorn antelope e 'Hobi'-like. A amostra detectada no presente estudo apresentou 99% de identidade com as cepas D32/00_HoBi (Número de acesso no GenBank: KP715144.1), detectada como contaminante de soro fetal bovino de origem brasileira (Schirrmeier et al 2004) e LV04/12 HoBi (Número de acesso no GenBank: KC465391.1), detectada em bovino com sintomatologia semelhante a doença das mucosas em Pombal, Paraíba, Brasil (Weber et al 2014). Portanto, a amostra viral detectada no presente estudo (denominada PB22487/12) está mais relacionada com o vírus 'Hobi'-like.O levantamento sorológico da propriedade revelou que 69,6% (16/23) dos animais foram soropositivos para o BVDV.…”

Section: Resultsunclassified

“…Alguns autores afirmam que o BVDV-3, em infecções naturais e experimentais, também está relacionado a síndromes respiratórias (Decaro et al 2011, 2012b, Larska et al 2012, abortos (Cortez et al 2006, Bianchi et al 2011, Decaro et al 2012a) e episódios de pirexia sem outras manifestações clínicas , de maneira que estas afecções são clinicamente semelhantes àquelas causadas pelo BVDV-1 e BVDV-2 (Decaro et al 2013, Weber et al 2014.…”

Section: Discussionunclassified

“…Geralmente o consumo hídrico de animais enfermos é inadequado para manter o conteúdo de água corporal normal, resultando em hemoconcentração, que aumenta o hematócrito e a proteína plasmática total (Lopes et al 2007). Decaro et al (2013) observaram apenas moderada linfopenia sem encontrar outras alterações significativas nos parâmetros hematológicos e bioquímicos de um bezerro PI por BVDV-3, enquanto Weber et al (2014) relataram leucopenia e neutrofilia nas mesmas circunstâncias. A maioria dos bovinos infectados apresentam leucopenia transitória e neutropenia, e em infecções persistentes, diferentemente do que ocorre na infecção aguda, a imunodepressão é induzida principalmente por alterações nas funções de linfó-citos, macrófagos e neutrófilos, pela afinidade do vírus por células de defesa, causando sua destruição e prejuízo funcional das células sobreviventes (Potgieter 2004).…”

Section: Discussionunclassified

“…Entretanto, alguns autores afirmam que a possibilidade de transmissão a partir de animais acometidos transitoriamente é mínima em relação ao PI, uma vez que a duração da infecção e quantidade de vírus por eles excretados é bem menor (Niskanen et al 2000, Niskanen et al 2002. Apesar da semelhança entre a cepa viral isolada neste relato com a do surto relatado por Weber et al (2014) em uma região próxima, o proprietário afirma que não adquiria animais provenientes daquele local.…”

Section: Discussionunclassified

“…Na propriedade havia ainda histórico de doença reprodutiva caracterizada por aborto e repetição de cio. Foi possível diagnosticar a presença de anticorpos contra o BVDV no rebanho e isolar em quatro animais estirpes semelhantes ao vírus 'Hobi'-like (Weber et al 2014). A crescente identificação de pestivirus atípicos em pelo menos três continentes tem suscitado grande preocupação de técnicos e pesquisadores em sanidade animal, pois tais agentes são recentes e suas epidemiologia, biologia e patogenia ainda são pouco conhecidas (Ridpath 2010, Ståhl & Alenius 2012.…”

Section: Introductionunclassified

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Detecção do virus ‘HoBi’-like (BVDV-3) em bovino no semiárido do Estado da Paraíba

et al. 2016

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RESUMO.-Objetivou-se descrever os aspectos clínicos e anatomopatológicos, e a identificação viral de um caso de infecção pelo vírus 'Hobi'-like (BVDV-3) em bovino do semiárido paraibano, Nordeste do Brasil. Um bovino, fêmea, três meses de idade, foi levado ao Hospital Veterinário da UFCG apresentando salivação, dificuldade de apreensão do teto, falta de apetite, fezes escuras e em pouca quantidade. Diante da piora do quadro clínico optou-se por sua eutaná-sia in extremis, seguida da realização da necropsia e coleta de material para histopatologia. Histologicamente, nas mucosas do trato digestivo, havia edema, degeneração balonosa, necrose e infiltrado inflamatório, que foi observado na face dorsal da língua e no seu epitélio mais profundo. The aim of this study was to describe the clinical and anatomopathological aspects, and the viral identification of a case of 'Hobi'-like (BVDV-3) vírus infection in cattle from the semiarid of Paraiba State, Northeastern Brazil. A female bovine, three months old, was sent to the UFCG's Veterinary Hospital presenting salivation, difficulty of ceiling seizure, lack of appetite, dark feces and in small amounts. Due to worsening of symptoms it was decided to in extremis euthanasia, followed by the necropsy and collection of material for histopathology. Histologically, in the mucous membranes of the digestive tract there were edema, ballooning degeneration, necrosis and inflammatory infiltrate, which was observed on the dorsal surface of the tongue and in its deepest epithelium. The immunohistochemical of skin biopsies of the extremity of the ear (ear notches) showed positive antigenic marking and by RT-PCR it was possible to detect viral RNA of BVDV in the serum, whose cytopathic effect in epithelial cells of bovine kidney lineage "Madin Darby bovine kidney" (MDBK)was not observed. The sequencing of the 5'NCR gene showed that the virus isolated was more related to 'Hobi'-like (BVDV-3). After confirming the diagnosis serum samples were collected from 23 animals for serology by indirect ELISA, and a seropositivity of 69.6% (16/23) was found. The identification of this new case of 'Hobi'-like infection in Paraiba reaffirms the need for regular BVDV monitoring in the region to early detection of infection in the herds and adoption of effective preventive and control measures.INDEX TERMS: Cattle, BVDV-3, pestivirus, viral identification.

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Section: Resultsunclassified

Section: Discussionunclassified

Section: Introductionunclassified

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Detecção do virus ‘HoBi’-like (BVDV-3) em bovino no semiárido do Estado da Paraíba

et al. 2016

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Genomic and antigenic relationships between two ‘HoBi’-like strains and other members of the Pestivirus genus

et al. 2017

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'HoBi'-like viruses comprise a putative new species within the genus Pestivirus of the family Flaviviridae. 'HoBi'-like viruses have been detected worldwide in batches of fetal calf serum, in surveillance programs for bovine pestiviruses and from animals presenting clinical signs resembling bovine viral diarrhea virus (BVDV)-associated diseases. To date, few complete genome sequences of 'HoBi'-like viruses are available in public databases. Moreover, detailed analyses of such genomes are still scarce. In an attempt to expand data on the genetic diversity and biology of pestiviruses, two genomes of 'HoBi'-like viruses recovered from Brazilian cattle were described and characterized in this study. Analysis of the whole genome and antigenic properties of these two new 'HoBi'-like isolates suggest that these viruses are genetically close to recognized pestiviruses. The present data provide evidence that 'HoBi'-like viruses are members of the genus Pestivirus and should be formally recognized as a novel species.

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Herd-level prevalence and risk factors for bovine viral diarrhea virus infection in cattle in the State of Paraíba, Northeastern Brazil

Fernandes

Nogueira

Stéfano

et al. 2015

Trop Anim Health Prod

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Serological surveys based on a planned sampling on bovine viral diarrhea virus (BVDV) infection in Brazilian cattle herds are scarce. A cross-sectional study was carried out to determine herd- and animal-level seroprevalences and to identify risk factors associated with herd-level seroprevalence for BVDV infection in the State of Paraíba, Northeastern Brazil, from September 2012 to January 2013. The state was divided into three sampling strata, and for each stratum, the prevalence of herds infected with BVDV and the prevalence of seropositive animals was estimated by a two-stage sampling survey. In total, 2443 animals were sampled from 478 herds. A virus-neutralization test was used for BVDV antibody detection. A herd was considered positive when at least one seropositive animal was detected. The herd- and animal-level prevalences in the State of Paraíba were 65.5% (95% confidence interval (CI) = 61.1-69.7%) and 39.1% (95% CI = 33.1-45.6%), respectively. The frequency of seropositive animals per herd ranged from 10 to 100% (median of 50%). The risk factors identified were as follows: more than six calves aged ≤12 months (odds ratio (OR) = 3.72; 95% CI = 2.08-6.66), animal purchasing (OR = 1.66; 95% CI = 1.08-2.55), pasture rental (OR = 2.15; 95% CI = 1.35-3.55), and presence of veterinary assistance (OR = 2.04; 95% CI = 1.10-3.79). Our findings suggest that the implementation of control and prevention measures among farmers, with the aim of preventing dissemination of the agent in the herds, is necessary. Special attention should be given to addressing the identified risk factors, such as sanitary control prior to animal purchasing and to discourage the pasture rental, as well as to encourage the vaccination in the herds.

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Clinical Presentation Resembling Mucosal Disease Associated with ‘HoBi’-like Pestivirus in a Field Outbreak

Cited by 54 publications

References 41 publications

Detecção do virus ‘HoBi’-like (BVDV-3) em bovino no semiárido do Estado da Paraíba

Detecção do virus ‘HoBi’-like (BVDV-3) em bovino no semiárido do Estado da Paraíba

Genomic and antigenic relationships between two ‘HoBi’-like strains and other members of the Pestivirus genus

Herd-level prevalence and risk factors for bovine viral diarrhea virus infection in cattle in the State of Paraíba, Northeastern Brazil

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