Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Song, Wenyan; Sun, Yingpu; Jin, Haixia; Xin, Zhi-Min; Su, Yingchun; Chian, Ri‐Cheng

doi:10.3109/19396368.2011.566666

Cited by 24 publications

(23 citation statements)

References 21 publications

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“…These encouraging results suggest that oocyte vitrification can be largely used for a panel of purposes including oocyte storing in cases of sperm retrieval failure [19], fertility preservation [20] or egg donation [3,13].…”

Section: Discussionmentioning

confidence: 97%

Oocyte vitrification: A local validation of the method

Boyer¹,

Montjean²,

Tourame³

et al. 2013

OJOG

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Is there a really need to validate oocyte vitrification technique in an ART laboratory before establishing it in daily practice? Validation of micromanipulationbased technique, in this case oocyte vitrification, is essential prior to enlarging its use to routine practice. Oocyte vitrification is a new worldwide used technique and legal recently in France. This micromanipulation needs to be performed by a skilled and experienced embryologist and requires an internal assessment in each ART unit before any wide use. We designed a prospective study, from September 2011 to July 2012, using sibling oocytes from women who recovered more than 12 Metaphase II oocytes. A part of freshly recovered oocytes underwent immediate ICSI while the remaining oocytes were vitrified. 87 couples undergoing ICSI were selected based on number of mature oocytes available on the recovery day after denudation. A part of fresh MII oocytes were microinjected and the others were vitrified using an open system (Cryotop ® ). The major criterion of interest was the number of embryo transferred/ number of Metaphase II ratio for after ICSI on fresh oocytes (42/211) versus vitrified/warmed oocytes (51/204) (p > 0.05). Secondary studied criteria were survival rate (80.5% ± 26.3%), fertilization rate (68.9 ± 33.5) and finally, cumulative pregnancy rate obtained in this study is 40.2%. One of the benefits of such practice is the limitation of embryo freezing. However, the study design delays oocytes warming cycles, due to pregnancies triggered by the transfer of fresh derived oocyte embryos and to the priority to transfer all the frozen embryos before starting oocytes warming. Moreover, no data is available about children' health. Oocyte vitrification represents not only a change in our daily practice to improve cumulative pregnancy rate but also a promising tool to develop egg banking and donation. Clinical Trials Registration number: 209 R02.

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Section: Discussionmentioning

confidence: 97%

Oocyte vitrification: A local validation of the method

Boyer¹,

Montjean²,

Tourame³

et al. 2013

OJOG

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“…Ovarian stimulation was performed with a standard long protocol in all female patients [10]. After complete down-regulation using decapeptyl (Germany), Gonal-F (FSH: follicle-stimulating hormone, Serono Company, Switzerland) was given on the third day of menstrual cycle.…”

Section: Ovarian Stimulationmentioning

confidence: 99%

“…There are reports about babies born using vitrified oocytes of the three types [8][9][10], but it is yet unclear which kind of oocytes is more suitable to vitrification. Moreover, at present, most studies about human oocyte vitrification are clinically retrospective [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Vitrification on Outcomes of In VivoMature, In Vitro-Mature and Immature Human Oocytes

Song

Peng

Jin

et al. 2016

Cell Physiol Biochem

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Key Words Vitrification • In vivo-mature human oocytes • In vitro-mature human oocytes • Immature human oocytesAbstract Background/Aims: To observe the effects of vitrification on spindle, zona pellucida, embryonic aneuploidy and DNA injury in in vivo-maruted, in vitro-mature and immature human oocytes. Methods: Between January 2009 and February 2015, 223 immature oocytes from 450 infertile patients, and 31 in vivo-mature oocytes from 3 infertile couples were collected. Of the 223 immature oocytes, 113 were used for in vitro culture before vitrification. Some oocytes were randomly divided into in vivo-mature group (group A, n = 15), in vitro-mature group (group B, n = 88) and immature group (group C, n = 85), and then the oocytes with spindle in these three groups after freezing-thawing were selected to use for Polscope imaging, embryonic aneuploidy screening and embryo development evaluation. Other oocytes were randomly divided into group A (n = 16), group B (n = 25) and group C (n = 25) for detecting DNA injury. Results: After thawing, spindle occurrence rate, spindle Retardance value, and cleavage rate were significantly higher in groups A and B than in group C (all P < 0.05), but there were no statistical differences in fertility rate, high-quality embryo rate, blastulation rate and aneuploidy rate amongst the three groups (all P > 0.05). Zona pellucida density (ZPD) was significantly lower in group A than in groups B and C both before and after vitrification (all P < 0.05). ZPD was significantly higher after thawing than before vitrification (all P < 0.05), but zona pellucida thickness (ZPT) was not significantly changed in all the three groups (all P > 0.05). Rate of comet cells was significantly lower in group A than in groups B and C (all P < 0.01). Comet tail was significantly longer in group C than in groups B and A (all P < 0.05). Conclusion:In vivo-and in vitro-mature human oocytes are more suitable to vitrification than immature human oocytes. Spindle Retardance value has more predictive value for embryonic development potential than ZPD and ZPT.

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“…Spare oocytes can be cryopreserved for a future attempt if the number of spermatozoa retrieved from the testis is insufficient to inject all available oocytes [Chian 2010;Song et al 2011]. Cryopreservation of testicular spermatozoa is already a widely used technique with which the repetition of surgical procedures for future ICSI attempts can be avoided [Carrel 2010;Friedler et al 1997;Küpker et al 2000;Revel and Mejia 2010;Wald et al 2006].…”

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confidence: 99%

Birth and clinical pregnancy from fresh and frozen oocytes fertilized with cryopreserved testicular spermatozoa

Zeke

Konc

Kanyó

et al. 2012

Systems Biology in Reproductive Medicine

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This is the first report showing a second clinical pregnancy of a couple who already have a baby from a previous frozen embryo transfer cycle when the embryos were generated from fresh oocytes that were fertilized by intracytoplasmic sperm injection (ICSI) using frozen testicular spermatozoa (the couple have unsuccessful fresh and frozen embryo transfer cycles). Fifty-two months after the first IVF/ICSI cycle the couple had their second IVF/ICSI cycle, but the collected oocytes (n=8) were frozen because no spermatozoa was obtained from the frozen testicular tissue samples which were cryopreserved prior to the first IVF/ICSI cycle. New testicular tissue samples were obtained and frozen. Finally, 58 months after the first IVF/ICSI cycle all of the 8 frozen oocytes of the couple were thawed and fertilized by ICSI using frozen testicular spermatozoa obtained from the newly cryopreserved testicular tissue. Three embryos were transferred and the couple has an ongoing pregnancy, which is in the 20(th) week of pregnancy. Our case report shows that: 1) developmentally competent embryos can be generated by ICSI of frozen-thawed testicular spermatozoa into both fresh and frozen human oocytes, and 2) clinical pregnancy and a healthy baby can be conceived from both frozen and fresh oocytes fertilized with cryopreserved testicular spermatozoa.

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Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Cited by 24 publications

References 21 publications

Oocyte vitrification: A local validation of the method

Oocyte vitrification: A local validation of the method

Effects of Vitrification on Outcomes of In VivoMature, In Vitro-Mature and Immature Human Oocytes

Birth and clinical pregnancy from fresh and frozen oocytes fertilized with cryopreserved testicular spermatozoa

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