Clinical optical coherence tomography combined with multiphoton tomography of patients with skin diseases

König, Karsten; Speicher, Marco; Bückle, Rainer; Reckfort, Julia; McKenzie, Gordon; Welzel, Julia; Koehler, Martin; Elsner, Peter; Kaatz, Martin

doi:10.1002/jbio.200910013

Cited by 97 publications

(62 citation statements)

References 27 publications

(19 reference statements)

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“…OCT was performed with a swept source centered at 1300 nm and provided simultaneous largearea screening of simple microsphere specimens to demonstrate the spatial coregistration in the small intestine and ear tissues of mouse models ex vivo. König et al 200 reported the first clinical study with a variety of skin diseases and disorders, such as skin cancer, psoriasis, hemangioma, connective tissue diseases, pigmented lesions, and autoimmune bullous skin diseases. Two different OCT systems were combined with MPT and dermoscopy to provide 2-D color images of the skin surface.…”

Section: Multimodal Oct and Mpmmentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

422

264

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Abstract. In the last 25 years, optical coherence tomography (OCT) has advanced to be one of the most innovative and most successful translational optical imaging techniques, achieving substantial economic impact as well as clinical acceptance. This is largely owing to the resolution improvements by a factor of 10 to the submicron regime and to the imaging speed increase by more than half a million times to more than 5 million A-scans per second, with the latter one accomplished by the state-of-the-art swept source laser technologies that are reviewed in this article. In addition, parallelization of OCT detection, such as line-field and full-field OCT, has shortened the acquisition time even further by establishing quasi-akinetic scanning. Besides the technical improvements, several functional and contrast-enhancing OCT applications have been investigated, among which the label-free angiography shows great potential for future studies. Finally, various multimodal imaging modalities with OCT incorporated are reviewed, in that these multimodal implementations can synergistically compensate for the fundamental limitations of OCT when it is used alone. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Multimodal Oct and Mpmmentioning

confidence: 99%

Optical coherence tomography today: speed, contrast, and multimodality

Drexler¹,

Li²,

Kumar³

et al. 2014

J. Biomed. Opt

422

264

View full text Add to dashboard Cite

show abstract

“…This system was capable of acquiring wide area mosaics ($1 Â 1 mm 2 ) of in vivo human skin. A clinical study investigating a variety of skin diseases such as psoriasis, pemphigus vulgaris and melanocytic lesions in vivo using the clinical MPT system, DermaInspect (JenLab GmbH, Jena, Germany) and two OCT systems (EX1301, Michelson diagnostics, UK; Callisto, Thorlabs GmbH, Germany) operated in two-dimensional (2D) mode, demonstrated the potential of MPT/OCT multimodal imaging technology for dermatological diagnostic applications [30]. However, more systematic studies using 3D multimodal MPT/OCT systems are required to identify and characterize dermatological disorders with high sensitivity and specificity.…”

Section: Biophotonicsmentioning

confidence: 99%

Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Alex

Weingast

Weinigel

et al. 2012

Journal of Biophotonics

Self Cite

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A preliminary clinical trial using state‐of‐the‐art multiphoton tomography (MPT) and optical coherence tomography (OCT) for three‐dimensional (3D) multimodal in vivo imaging of normal skin, nevi, scars and pathologic skin lesions has been conducted. MPT enabled visualization of sub‐cellular details with axial and transverse resolutions of <2 μm and <0.5 μm, respectively, from a volume of 0.35 × 0.35 × 0.2 mm3 at a frame rate of 0.14 Hz (512 × 512 pixels). State‐of‐the‐art OCT, operating at a center wavelength of 1300 nm, was capable of acquiring 3D images depicting the layered architecture of skin with axial and transverse resolutions ∼8 μm and ∼20 μm, respectively, from a volume of 7 × 3.5 × 1.5 mm3 at a frame rate of 46 Hz (1024 × 1024 pixels). This study demonstrates the clinical diagnostic potential of MPT/OCT for pre‐screening relatively large areas of skin using 3D OCT to identify suspicious regions at microscopic level and subsequently using high resolution MPT to obtain zoomed in, sub‐cellular level information of the respective regions (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…A combined system could perform a fast OCT scan to find a region of interest, which would subsequently be examined in detail with MPT. In a recent study, König et al demonstrated with correlated OCT and MPT images of skin cancer lesions diagnostic potential of combining these modalities [252]. Already in 2006, Vinegoni et al presented a combined MPT/OCM system that provides overlaid morphologic and spectroscopic images of different samples [253].…”

Section: Combination Of Modalitiesmentioning

confidence: 99%

Optical coherence tomography—current technology and applications in clinical and biomedical research

et al. 2011

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Optical coherence tomography (OCT) is a non-invasive imaging technique providing real-time twoand three-dimensional images of scattering samples with micrometer resolution. Mapping the local reflectivity, OCT visualizes the morphology of the sample. In addition, functional properties such as birefringence, motion or the distribution of certain substances can be detected with high spatial resolution. The main field of application is bio-medical imaging and diagnostics. In ophthalmology, OCT is accepted as a clinical standard for diagnosing and monitoring treatment of a number of retinal diseases, and OCT is becoming an important instrument for clinical cardiology. New applications are emerging in various medical fields, e. g. early-stage cancer detection, surgical guidance, and early diagnosis of musculoskeletal diseases. OCT has also proven its value as a tool for developmental biology. The number of companies involved in manufacturing OCT systems has increased substantially during the last years-especially due to its success in opthalmology-and this technology can be expected to continue to spread into various fields of application.

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Clinical optical coherence tomography combined with multiphoton tomography of patients with skin diseases

Cited by 97 publications

References 27 publications

Optical coherence tomography today: speed, contrast, and multimodality

Optical coherence tomography today: speed, contrast, and multimodality

Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Optical coherence tomography—current technology and applications in clinical and biomedical research

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