Clinical mastitis in ewes; bacteriology, epidemiology and clinical features

Mørk, Torill; Waage, S.; Tollersrud, Tore; Kvitle, Bjørg; Sviland, Ståle

doi:10.1186/1751-0147-49-23

Cited by 74 publications

(67 citation statements)

References 27 publications

(31 reference statements)

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“…This low incidence might be due to the lower diffusion of Gram-negative bacteria in sheep than in cattle, where E.coli is considered a major pathogen causing mastitis in cattle [50]. No bacteria isolated in 15(9.4%) samples from such cases, nearly an equivalent percent was mentioned by [46]. This might be related to the infection caused by bacteria other than the isolated ones or due to yeast, fungus or even mycoplasma species infections.…”

Section: Discussionmentioning

confidence: 99%

Clinical Mastitis in Sheep Smallholder System in Sharkia Province: Bacteriological Studies, In Vitro Antibiogram Profile and Mineral Picture

2018

Adv. Environ. Biol

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Section: Discussionmentioning

confidence: 99%

Clinical Mastitis in Sheep Smallholder System in Sharkia Province: Bacteriological Studies, In Vitro Antibiogram Profile and Mineral Picture

2018

Adv. Environ. Biol

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“…aureus has been reported to be the most common aetiological agent causing clinical mastitis (29-65% of isolated bacteria) while CNS (3-19%), E. coli (3-19%) and M. haemolytica (1.5-4.3%) have also been less frequently observed 2,[25][26][27] . In the present study also S. aureus was found to be the most frequently isolated species while other bacteria species such as M. haemolytica, CNS and E. coli was isolated less frequently in agreement with those reported by other researchers 2,25,28 . In the present study no growth of bacteria could be detected in 5.3% of the samples by using bacterial examination.…”

Section: Discussionmentioning

confidence: 99%

“…Nocardia genera 1,2 . In sheep production, mastitis has a major economical impact due to reduced milk yield and quality as well as increased use of drugs and veterinary services 3 .…”

Section: Mannheimia (Pasteurella) Haemolytica Escherichia Coli Closmentioning

confidence: 99%

Identifying the Bacteria Causing Ovine Gangrenous Mastitis and Detection of Staphylococcus aureus in Gangrenous Milk by PCR

Tel¹,

Bozkaya²

2012

Kafkas Univ Vet Fak Derg

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SummaryThe objective of this study was to identify bacteria causing gangrenous ovine mastitis as well as to detect and genotype Staphylococcus aureus in milk samples by using polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP) of coagulase gene. A total of 264 milk samples were collected from Awassi sheep with gangrenous mastitis raised in Sanliurfa province of Turkey. By using bacteriological examination Staphylococcus aureus (75%), Mannheimia haemolytica (7.2%), coagulase negative staphylococci (6.43%), Escherichia coli (4.55%), Corynebacterium spp. (0.76%) and Actinomyces pyogenes (0.76%) were identified. Bacterial DNA was isolated from 155 randomly selected milk sample. By PCR amplification of coagulase gene S. aureus was detected in 91.6% of the milk samples. Three different amplicons of 420 (0.7%), 580 (65.3%) and 780 (34%) bp band sizes, each showing a specific RFLP profile were observed. In conclusion, S. aureus was found to be mostly responsible for gangrenous mastitis in sheep raised in this region. S. aureus in milk samples could be more rapidly detected by PCR method. Two coa genotype of S. aureus were found to be common suggesting that limited number of strains were responsible for most cases of gangrenous mastitis in the region under the study. Keywords: Coa gene, Gangrenous mastitis, PCR, Sheep Staphylococcus aureus Koyunlarda Gangrenöz Mastitise Neden Olan Bakterilerin İdentifikayonu ve Gangrenli Sütlerdeki Staphylococcus aureus'un PCR ile Tespit Edilmesi ÖzetBu çalışmanın amacı, koyun gangrenöz mastitisine neden olan bakterilerin identifiye edilmesi ve süt örneklerindeki Staphylococcus aureus'un polimeraz zincir reaksiyonu (PCR) ile tespit edilerek, koagülaz geni yönünden restriksiyon fragman uzunluğu polimorfizminin (RFLP) belirlenmesidir. Bu amaçla Şanlıurfa ilinde yetiştirilmekte olan gangrenöz mastitisli İvesi koyunlarından 264 adet süt örneği toplanmıştır. Bakteriyolojik incelemede Staphylococcus aureus (%75), Mannheimia haemolytica (%7.2), koagulaz negatif stafilakok (%6.43), Escherichia coli (%4.55), Corynebacterium spp. (%0.76) ve Actinomyces pyogenes (%0.76) identifiye edildi. Tesadüfi olarak seçilen 155 süt örneğinden bakteriyel DNA izole edildi. Koagulaz geni kullanılarak yapılan PCR amplifikasyonu ile örneklerin %91.6'sında S. aureus tespit edildi. Herbiri spesifik RFLP profili gösteren 420 (%0.7), 580 (%65.3) ve 780 (%34) baz çifti uzunluğunda 3 farklı PCR ürünü gözlendi. Sonuç olarak, S. aureus'un, bölgede yetiştirilen koyunlarda gangrenöz mastitislerden büyük oranda sorumlu olduğu bulunmuştur. S. aureus'un süt örneklerinden izole edilen DNA kullanılarak PCR metoduyla kolaylıkla tespit edilebileceği, iki coa genotipinin yaygın olduğu ve sınırlı sayıda suşun bölgedeki gangrenöz mastitis olgularının çoğundan sorumlu olduğu kanaatine varılmıştır.

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“…(Spanu et al, 2011), Pseudomonas spp. (Leitner and Krifucks, 2007), Enterobacteriaceae (Fthenakis et al, 2004;Mork et al, 2007), Listeria monocytogenes (Brugère-Picoux, 2008) and other bacteria have been found to be related with mastitis in dairy ewes (Table 1).…”

Section: Etiologymentioning

confidence: 99%

“…However, some authors have reported outbreaks where the incidence of clinical mastitis ranged from 30% to 50% (Lafi et al, 1998;Calavas et al, 1998), with an increased incidence of clinical mastitis being observed in the early lactation until weaning (Mork et al, 2007;Arsenault et al, 2008;Gougoulis et al, 2008). In the case of subclinical mastitis, a prevalence ranging from 5% to 30% has been found Contreras et al, 2003;Berthelot et al, 2006).…”

Section: Epidemiologymentioning

confidence: 99%

Predisposing factors and control of bacterial mastitis in dairy ewes

Bramis

Gelasakis

Kiossis

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. Bacterial mastitis is a major health problem in dairy sheep worldwide. It is associated with reduced milk yield and occasionally involuntary culling of affected ewes, as well as insufficient growth and mortality of lambs. In general, the incidence of clinical mastitis in ewes during lactation is lower than 5%. However, the prevalence of subclinical mastitis is variable ranging from 10-30% or more. In clinical cases of bacterial intramammary infections, Staphylococcus aureus is dominant. In the case of subclinical mastitis the prevailing isolates are Coagulase-Negative Staphylococci (CNS). Moreover, predisposing factors related to the environment, genetics, udder morphology and nutrition, are likely to contribute to the occurrence of mastitis. Hence, control of the latter factors and bacteriological contamination during milking are the main preventive measures. The notion is that detection of the ovine genome regions involved in mastitis resistance will also facilitate the most effective control of mastitis in flock level. Appropriate genetic selection, together with the implementation of preventive measures, could reduce the negative consequences of bacterial mastitis.

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Clinical mastitis in ewes; bacteriology, epidemiology and clinical features

Abstract: Background: Clinical mastitis is an important disease in sheep. The objective of this work was to identify causal bacteria and study certain epidemiological and clinical features of clinical mastitis in ewes kept for meat and wool production.

Cited by 74 publications

References 27 publications

Clinical Mastitis in Sheep Smallholder System in Sharkia Province: Bacteriological Studies, In Vitro Antibiogram Profile and Mineral Picture

Clinical Mastitis in Sheep Smallholder System in Sharkia Province: Bacteriological Studies, In Vitro Antibiogram Profile and Mineral Picture

Identifying the Bacteria Causing Ovine Gangrenous Mastitis and Detection of Staphylococcus aureus in Gangrenous Milk by PCR

Predisposing factors and control of bacterial mastitis in dairy ewes

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