Clinical Manifestations of Zika Virus Infection, Rio de Janeiro, Brazil, 2015

Cerbino-Neto, José; Mesquita, Emersom Cicilini; Souza, Thiago Moreno L.; Parreira, Viviane Gomes; Wittlin, Bernardo Bastos; Durovni, Betina; Lemos, Maria Cristina Ferreira; Vizzoni, AG; Filippis, Ana María Bispo de; Sampaio, Simone Alves; Gonçalves, Bianca de Santis; Bozza, Fernando A.

doi:10.3201/eid2207.160375

Cited by 79 publications

(69 citation statements)

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“…ZIKV infection in adult humans results in conjunctivitis in ϳ50% of symptomatic infections (32)(33)(34), with uveitis having been diagnosed in multiple patients (35,36). ZIKV replication in eye-associated tissues has been reported in infected humans, with confirmation of viral RNA and infectious virus in conjunctival fluid (37).…”

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confidence: 99%

Animal Models of Zika Virus Infection, Pathogenesis, and Immunity

Morrison

Diamond

2017

J Virol

232

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Zika virus (ZIKV) is an emerging mosquito-transmitted flavivirus that now causes epidemics affecting millions of people on multiple continents. The virus has received global attention because of some of its unusual epidemiological and clinical features, including persistent infection in the male reproductive tract and sexual transmission, an ability to cross the placenta during pregnancy and infect the developing fetus to cause congenital malformations, and its association with Guillain-Barré syndrome in adults. This past year has witnessed an intensive effort by the global scientific community to understand the biology of ZIKV and to develop pathogenesis models for the rapid testing of possible countermeasures. Here, we review the recent advances in and utility and limitations of newly developed mouse and nonhuman primate models of ZIKV infection and pathogenesis.

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confidence: 99%

Animal Models of Zika Virus Infection, Pathogenesis, and Immunity

Morrison

Diamond

2017

J Virol

232

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“…Originally discovered in the Zika forest of Uganda in 1947 (1), ZIKV has been neglected for the past several decades. Since 2007, it has successfully expanded into the Pacific (2)(3)(4)(5) and the Americas (6)(7)(8), resulting in significant outbreaks and epidemics. More importantly, ZIKV infection was unexpectedly linked to congenital defects and severe neurological complications, including microcephaly (9)(10)(11)(12) and Guillain-Barré syndrome (3,13).…”

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confidence: 99%

Characterization of cis -Acting RNA Elements of Zika Virus by Using a Self-Splicing Ribozyme-Dependent Infectious Clone

Liu

Huang

et al. 2017

J Virol

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Zika virus (ZIKV) has caused significant outbreaks and epidemics in the Americas recently, raising global concern due to its ability to cause microcephaly and other neurological complications. A stable and efficient infectious clone of ZIKV is urgently needed. However, the instability and toxicity of flavivirus cDNA clones in Escherichia coli hosts has hindered the development of ZIKV infectious clones. Here, using a novel self-splicing ribozyme-based strategy, we generated a stable infectious cDNA clone of a contemporary ZIKV strain imported from Venezuela to China in 2016. The constructed clone contained a modified version of the group II self-splicing intron P.li.LSUI2 near the junction between the E and NS1 genes, which were removed from the RNA transcripts by an easy-to-establish in vitro splicing reaction. Transfection of the spliced RNAs into BHK-21 cells led to the production of infectious progeny virus that resembled the parental virus. Finally, potential cis-acting RNA elements in ZIKV genomic RNA were identified based on this novel reverse genetics system, and the critical role of 5=-SLA promoter and 5=-3= cyclization sequences were characterized by a combination of different assays. Our results provide another stable and reliable reverse genetics system for ZIKV that will help study ZIKV infection and pathogenesis, and the novel self-splicing intron-based strategy could be further expanded for the construction of infectious clones from other emerging and reemerging flaviviruses.IMPORTANCE The ongoing Zika virus (ZIKV) outbreaks have drawn global concern due to the unexpected causal link to fetus microcephaly and other severe neurological complications. The infectious cDNA clones of ZIKV are critical for the research community to study the virus, understand the disease, and inform vaccine design and antiviral screening. A panel of existing technologies have been utilized to develop ZIKV infectious clones. Here, we successfully generated a stable infectious clone of a 2016 ZIKV strain using a novel self-splicing ribozyme-based technology that abolished the potential toxicity of ZIKV cDNA clones to the E. coli host. Moreover, two crucial cis-acting replication elements (5=-SLA and 5=-CS) of ZIKV were first identified using this novel reverse genetics system. This novel self-splicing ribozyme-based reverse genetics platform will be widely utilized in future ZIKV studies and provide insight for the development of infectious clones of other emerging viruses.

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“…The 35 Brazilian neonates with microcephaly delivered by women who were suspected to have developed Zika virus infection during pregnancy were associated with congenital abnormalities, such as congenital The data are cited from several studies. 4,13,14,44,53,54 clubfoot, joint contracture or microphthalmia, other than microcephaly. 35 Additionally, cases with retinal or auditory abnormality have been reported, [35][36][37] which have also been observed in neonates without microcephaly.…”

Section: Complicationsmentioning

confidence: 99%

“…The clinical symptoms of Zika virus infection are shown in Table 2. 4 Generally, most cases have mild symptoms and rarely require hospitalization. Few fatal cases attributable to Zika virus infection have been reported.…”

Section: Clinical Symptomsmentioning

confidence: 99%

“…3 The infection has an incubation period of 2-7 days, with symptoms such as fever (including low-grade fever), headache, joint pain, muscle pain, hyperemic bulbar conjunctiva and skin rash. 4 The occurrence of Guillain-Barr e syndrome (GBS) has been reported, after Zika virus infection, while GBS is considered a rare complication of the infection. 5 In addition, there is an association between Zika virus infection during pregnancy and an increased risk of microcephaly in the fetus.…”

Section: Introductionmentioning

confidence: 99%

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Zika virus infection: Clinical overview with a summary of Japanese cases

Kutsuna

2017

Clinical & Exp Neuroim

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Zika virus infection is a mosquito-borne infection caused by the Zika virus, a member of the genus Flavivirus in the family Flaviviridae. The infection is transmitted to humans mainly by Aedes aegypti and Aedes albopictus mosquitoes. Sexual contact has also been reported to cause infection. It can be diagnosed based on the detection of the virus from blood or urine samples, detection of the viral genes by reverse transcription polymerase chain reaction, positive conversion of immunoglobulin M or neutralizing antibodies, or a significant increase in antibody titer in paired sera. No effective antiviral drugs or vaccines are currently available. On rare occasions, individuals infected with the Zika virus subsequently develop Guillain-Barr e syndrome. In addition, pregnant women infected with the Zika virus can have an increased risk of fetal microcephaly, which has been recognized as a social problem. Finally, I summarized Zika virus infection in Japan as of June 2017.

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Clinical Manifestations of Zika Virus Infection, Rio de Janeiro, Brazil, 2015

Cited by 79 publications

References 8 publications

Animal Models of Zika Virus Infection, Pathogenesis, and Immunity

Animal Models of Zika Virus Infection, Pathogenesis, and Immunity

Characterization of cis -Acting RNA Elements of Zika Virus by Using a Self-Splicing Ribozyme-Dependent Infectious Clone

Zika virus infection: Clinical overview with a summary of Japanese cases

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