Clinical implication of autoantibodies in patients with systemic rheumatic diseases

Satoh, Minoru; Chan, Edward K. L.; Sobel, Eric S.; Kimpel, Donald L.; Yamasaki, Yoshioki; Narain, Sonali; Mansoor, Rizwan; Reeves, Westley H.

doi:10.1586/1744666x.3.5.721

Cited by 65 publications

(86 citation statements)

References 95 publications

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“…As shown in Table 4, the number of sera scored in our laboratory as definitely ANA positive by IFA during the year 2011, represented only 2.3% of the total examined, while up to 7.4% (3.2 times more) of samples were positive by IB only (these sera were positive at 1:10 dilution when tested by IFA, but were negative at the 1:100 dilution). This finding does not contradict to the statement that IFA is the best approach for ANA screening (Satoh et al, 2007 (Bradwell et al, 1995;Verstegen et al, 2009). According to our experience, this relationship is true at least with a probability of 55%.…”

Section: Remarks To Ana Diagnosticsupporting

confidence: 58%

Detection of autoantibodies by indirect immunofluorescence and related techniques: the pathologist´s view

Rajčáni

Korinkova²,

Bencat

2015

MRAJ

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The increasing importance of autoimmunity based diseases attracts the interest of pathologists, for whom the wide acceptance of indirect fluorescent antibody (IFA) technique at autoantibody screening is a relevant diagnostic approach. The IFA method has gained perfection by introducing the so called diagnostic mosaics, consisting of various tissue sections (primate and/or mammalian in origin), of HEp-2 cells, of microdots composed of specific purified antigen(s) and/or of transfected HEK cells expressing the target autoantigens. As result of this, the IFA technique dominates not only in the detection of anti-nuclear antibodies

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Section: Remarks To Ana Diagnosticsupporting

confidence: 58%

Detection of autoantibodies by indirect immunofluorescence and related techniques: the pathologist´s view

Rajčáni

Korinkova²,

Bencat

2015

MRAJ

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“…A marker of human systemic lupus erythamosus is the presence of high-titer IgG autoantibodies against nuclear autoantigens, especially dsDNA (35,36). Similarly, aged Fas lpr or B-cell-specific fas-deficient mice produce excessive amounts of autoantibodies directed against dsDNA or ssDNA autoantigens (5, 32).…”

Section: Significancementioning

confidence: 99%

Deletion of microRNA-155 reduces autoantibody responses and alleviates lupus-like disease in theFas^lprmouse

Thai

Patterson

Pham

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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regulates antibody responses and subsequent B-cell effector functions to exogenous antigens. However, the role of miR-155 in systemic autoimmunity is not known. Using the death receptor deficient (Fas lpr ) lupus-prone mouse, we show here that ablation of miR-155 reduced autoantibody responses accompanied by a decrease in serum IgG but not IgM anti-dsDNA antibodies and a reduction of kidney inflammation. MiR-155 deletion in Fas lpr B cells restored the reduced SH2 domain-containing inositol 5′-phosphatase 1 to normal levels. In addition, coaggregation of the Fc γ receptor IIB with the B-cell receptor in miR-155 −/− -Fas lpr B cells resulted in decreased ERK activation, proliferation, and production of switched antibodies compared with miR-155 sufficient Fas lpr B cells. Thus, by controlling the levels of SH2 domaincontaining inositol 5′-phosphatase 1, miR-155 in part maintains an activation threshold that allows B cells to respond to antigens.ERK pathways | SHIP-1 M icroRNA-155 (miR-155) plays a critical role in the generation of effective antibody responses to exogenous antigenic challenges in mice (1-3). MiR-155 levels have been reported to be elevated in B but low in T cells from patients with systemic lupus erythamosus (4), yet it is not known whether miR-155 controls autoimmune responses and the expression of related pathology.Mice harboring ubiquitous or B-cell-specific ablation of the death receptor Fas develop a severe lupus-like disease. B-cellspecific deletion of the death receptor (fas −/− ) fas −/− mice develop an excessive germinal center (GC)-derived IgG autoantibody deposition in their kidneys and succumb to renal failure (5). It has been suggested that loss of tolerance in lpr mice results from the down-regulation of the low-affinity IgG inhibitory receptor FcγRIIB (Fc γ receptor IIB), thereby rendering their B cells incapable of terminating stimulatory signals delivered by autoantigen-containing immune complexes (6-8). However, the mechanisms whereby lack of FcγRIIB engagement would lead to autoimmunity, and whether additional factors contribute to autoimmunity, are still unclear.The SH2 domain-containing inositol 5′-phosphatase 1 (SHIP-1) phosphatase acts downstream of inhibitory cell-surface receptors (9-12), including the FcγRIIB, which is essential in opposing B-cell activation signals in mice and humans (13,14). FcγRIIB inactivation has been implicated in the development of autoreactive GC B cells and plasma cells (15), as well as in the regulation of the persistence and longevity of bone marrow plasma cells (16). After coligation of the FcγRIIB with the B-cell receptor (BCR), FcγRIIB recruits SHIP-1 to the plasma membrane, where it negatively regulates cell survival, Ca 2+ -dependent effector functions, and ERK activation, thus controlling cell proliferation, anergy, and apoptosis (17-23). As a consequence of these wideranging activities, germ-line or B-cell-specific deletion of FcγRIIB or SHIP-1 in mice results in a severe lupus-like disease characterized by high-titer serum IgG antinucl...

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“…IIF allows the detection of a large number of nuclear and cytoplasm antigens, however, the IIF assay has some downsides. It is laborious and time consuming due to the large number of serial dilutions and visual determination of the staining patterns [7]. The assay is subjective as it relies heavily on human interpretation [8].…”

Section: Introductionmentioning

confidence: 99%

Predictive autoimmunity using autoantibodies: screening for anti-nuclear antibodies

Pérez

Gilburd

Cabrera-Marante³

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background: Early detection of antinuclear antibodies (ANA) in asymptomatic subjects is useful to predict autoimmune diseases years before diagnosis. ANA have been determined by indirect immunofluorescence (IIF) using human epithelial type 2 (HEp-2) cells, which is considered the gold standard technique. Multiplex technology (BioPlex ANA Screen) has been introduced for ANA evaluation in recent years. Nevertheless, concordance between BioPlex and IIF is low and there is no harmonization between both methods for detection of autoantibodies. This study has aimed to clarify the clinical significance of autoantibodies detected by BioPlex ANA Screen in subjects with undiagnosed clinical suspicion of autoimmune disease and to determine the predictive value of autoantibodies detected by BioPlex ANA Screen. Methods: A 3-year follow-up study was performed of 411 subjects without a clear diagnosis of autoimmune diseases in whom autoantibodies were detected by BioPlex ANA Screen that were negative by IIF on HEp-2 cells.

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Clinical implication of autoantibodies in patients with systemic rheumatic diseases

Cited by 65 publications

References 95 publications

Detection of autoantibodies by indirect immunofluorescence and related techniques: the pathologist´s view

Detection of autoantibodies by indirect immunofluorescence and related techniques: the pathologist´s view

Deletion of microRNA-155 reduces autoantibody responses and alleviates lupus-like disease in theFas^lprmouse

Predictive autoimmunity using autoantibodies: screening for anti-nuclear antibodies

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Clinical implication of autoantibodies in patients with systemic rheumatic diseases

Cited by 65 publications

References 95 publications

Detection of autoantibodies by indirect immunofluorescence and related techniques: the pathologist´s view

Detection of autoantibodies by indirect immunofluorescence and related techniques: the pathologist´s view

Deletion of microRNA-155 reduces autoantibody responses and alleviates lupus-like disease in theFaslprmouse

Predictive autoimmunity using autoantibodies: screening for anti-nuclear antibodies

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Deletion of microRNA-155 reduces autoantibody responses and alleviates lupus-like disease in theFas^lprmouse