Clinical <i>Trypanosoma rangeli</i> infection as a complication of Chagas‘ disease

Guhl, Felipe; Hudson, L.; Marinkelle, C. J.; Jaramillo, Carlos; Bridge, Dacie R.

doi:10.1017/s0031182000055827

Cited by 57 publications

(39 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, none of the sera from T. rangeli infected children showed a positive reaction with the T. cruzi IFAT, ELISA or with the Western blot tests used. In agreement with our results, other studies have reported no recognition of T. cruzi antigens by T. rangeli infected human sera (Guhl et al 1987, Hudson et al 1988, Vasquez et al 1997). It appears, therefore, that most of the T. rangeli human infections do not raise enough cross-reacting an- a: reactivity in at least two of the three serological tests used.…”

supporting

confidence: 93%

Predominance of Trypanosoma rangeli infection in children from a Chagas disease endemic area in the west-shore of the Panama canal

Saldaña

Samudio

Miranda

et al. 2005

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

A total of 206 serum samples from children (3-14 years old) Human Trypanosoma cruzi and T. rangeli infections have, for a long time, been recognized as endemic in Panama (Sousa & Johnson 1971, 1973, Sousa & Saldaña 1994. However, currently available, seroepidemiological information is insufficient to convey an acceptable picture of the prevalence of Chagas disease in the country. Of special interest is the unclear role of T. rangeli on human infections. This trypanosome is considered totally benign (Cuba Cuba 1998, Guhl & Vallejo 2003 but with significant immunological consequences on T. cruzi infection (Zuñiga et al. 1997, Palau et al 2003, Basso et al. 2004. It is therefore necessary to update the information concerning human trypanosome infections in endemic regions from Panama.During 2004, a descriptive, parasitological, and seroprevalence study on human trypanosome infections was conducted in the Amador County, La Chorrera District, in the Province of Panama. Four neighboring rural communities, previously recognized as endemic for T. cruzi infection, were selected: Lagartera Grande, Los Hules, Lagarterita, and Las Pavas. The estimated population of these four areas is 1000 inhabitants. In these localities the ecoepidemiological characteristics are favorable for trypanosomes transmission and, at present, no organized vector control measures have been established by the health authorities. The climate in this region is tropical and comprises pasture lands where the presence of the royal palm tree, Attalea butyracea, is abundant. A. butyracea is the primary biotope of Rhodnius pallescens, the main vector of T. cruzi and T. rangeli in Panama. Also, this palm tree is considered a good ecological indicator of Chagas disease risk areas (Romaña et al. 2003).To determine the prevalence of anti-T. cruzi antibodies and blood trypanosomes in these localities, 206 apparently healthy children aged between 3 and 14 years old were investigated. This sample size represents 80% of the total number of children in this age range inhabiting the studied sites. Blood samples were obtained by venipuncture from each child, informed written consent was obtained from all parents or legal guardians. Hemoculture of samples was performed as described by Vasquez et al. (1997). To corroborate and identify the species of trypanosomes isolated, a duplex PCR assay (Chiurillo et al. 2003) followed by dot blot hybridization with radiolabeled oligonucleotide probes specific for T. cruzi and T. rangeli was performed on initially microscopically positive hemocultures.Sera samples were at first screened for the presence of anti-T. cruzi antibodies using an indirect immunofluorescence antibody test (IFAT) with a local T. cruzi isolate (Burunga strain) as antigen, according to the technique described by Guhl and Nicholls (2001). The sensitivity and specificity of this assay is of 95.2 and 96.3%, respectively. Samples with circulating anti-T. cruzi antibodies in the screening test were further submitted to a commercial recombinant ELISA (ELISA, Cha...

show abstract

supporting

confidence: 93%

Predominance of Trypanosoma rangeli infection in children from a Chagas disease endemic area in the west-shore of the Panama canal

Saldaña

Samudio

Miranda

et al. 2005

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…In this sense, recent data have shown that complete or partial immunity against T. cruzi can be obtained in animal models previously immunized with low virulent T. cruzi strains 5,9 or with T. rangeli 1,29 . The fact that more than one T. cruzi strain 10,16 and even more than one Trypanosoma species 6,23 can be transmitted by R. pallescens in Panama makes this possibility feasible. Undoubtedly, this hypothesis needs to be further evaluated and other aspects responsible for the large spectrum of Chagas disease manifestations, such as host genetic factors and local T. cruzi isolates virulence, should also be taken into consideration.…”

Section: Discussionmentioning

confidence: 99%

Eco-epidemiological aspects of Trypanosoma cruzi, Trypanosoma rangeli and their vector (Rhodnius pallescens) in Panama

Vasquez

Samudio

Saldaña

et al. 2004

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYThe eco-epidemiology of T. cruzi infection was investigated in the Eastern border of the Panama Canal in Central Panama. Between 1999 and 2000, 1110 triatomines were collected: 1050 triatomines (94.6%) from palm trees, 27 (2.4%) from periurban habitats and 33 (3.0%) inside houses. All specimens were identified as R. pallescens. There was no evidence of vector domiciliation. Salivary glands from 380 R. pallescens revealed a trypanosome natural infection rate of 7.6%, while rectal ampoule content from 373 triatomines was 45%. Isoenzyme profiles on isolated trypanosomes demonstrated that 85.4% (n = 88) were T. cruzi and 14.6% (n = 15) were T. rangeli. Blood meal analysis from 829 R. pallescens demonstrated a zoophilic vector behavior, with opossums as the preferential blood source. Seroprevalence in human samples from both study sites was less than 2%. Our results demonstrate that T. cruzi survives in the area in balanced association with R. pallescens, and with several different species of mammals in their natural niches. However, the area is an imminent risk of infection for its population, consequently it is important to implement a community educational program regarding disease knowledge and control measures.

show abstract

“…As positive controls, we used 100 pg of DNA from T. cruzi Berenice (Type 1) and Dm28 (Type 2). Negative controls were 100 pg of DNA from Leishmania braziliensis (42) and T. rangeli (43,44).…”

Section: Phenotypic and Genotypic Characterization Of T Cruzi-like Imentioning

confidence: 99%

Emerging Chagas Disease: Trophic Network and Cycle of Transmission ofTrypanosoma cruzifrom Palm Trees in the Amazon

Teixeira

Monteiro

Rebêlo

et al. 2001

Emerg. Infect. Dis.

View full text Add to dashboard Cite

Clinical Trypanosoma rangeli infection as a complication of Chagas‘ disease

Cited by 57 publications

References 10 publications

Predominance of Trypanosoma rangeli infection in children from a Chagas disease endemic area in the west-shore of the Panama canal

Predominance of Trypanosoma rangeli infection in children from a Chagas disease endemic area in the west-shore of the Panama canal

Eco-epidemiological aspects of Trypanosoma cruzi, Trypanosoma rangeli and their vector (Rhodnius pallescens) in Panama

Emerging Chagas Disease: Trophic Network and Cycle of Transmission ofTrypanosoma cruzifrom Palm Trees in the Amazon

Contact Info

Product

Resources

About