Clinical Hæmatology

Dacie, J. V.; Woodliff, H.J.; Holman, R.A.

doi:10.1016/s0140-6736(69)92086-8

Cited by 134 publications

(155 citation statements)

References 2 publications

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“…Glutathione peroxidase and superoxide dismutase were analysed by kit provided by RANDOX Laboratories Limited, United Kingdom. Blood was also analysed for haemoglobin (Dacie and Lewis 1975) and fasting blood glucose (Trinder 1969).…”

Section: Methodsmentioning

confidence: 99%

Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women

2012

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Menopause is a gradual three-stage process that concludes with the end of periods and reproductive life. The antioxidant enzyme system get affected in postmenopause due to deficiency of estrogen, which has got antioxidant properties. The objective of the present study was therefore, to analyze the effect of supplementation of drumstick and amaranth leaves powder on blood levels of antioxidant and marker of oxidative stress. Ninety postmenopausal women aged 45-60 years were selected and divided into three groups viz. Group I, II and III having thirty subjects in each group. The subjects of group II and III were supplemented daily with 7 g drumstick leaves powder (DLP) and 9 g amaranth leaves powder (ALP), respectively for a period of 3 months in their diet. The subjects of group I was not given supplementation. Serum retinol, serum ascorbic acid, glutathione peroxidase, superoxide dismutase and malondialdehyde were analyzed before and after supplementation. Fasting blood glucose and haemoglobin level of the subjects were also analyzed. The data revealed that supplementation of DLP and ALP significantly increased serum retinol (8.8 % and 5.0 %), serum ascorbic acid (44.4 % and 5.9 %), glutathione peroxidase (18.0 % and 11.9 %), superoxide dismutase (10.4 % and 10.8) whereas decrease in marker of oxidative stress i.e. malondialdehyde (16.3 % and 9.6 %) in postmenopausal women of group II and group III, respectively. A significant (p≤0.01) decrease was also observed in fasting blood glucose level (13.5 % and 10.4 %) and increase in haemoglobin (17.5 % and 5.3 %) in group II and group III, respectively. The results indicated that these plants possess antioxidant property and have therapeutic potential for the prevention of complications during postmenopause.

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Section: Methodsmentioning

confidence: 99%

Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women

2012

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“…Packed cell volume (%) was measured using the microcapillary reader technique, and corrected by 1.5% for plasma trapped within erythrocytes [24]. Haemoglobin (g/dl) was measured using a β-haemoglobin photometer (Hemocue, Angelholm, Sweden).…”

Section: Analysesmentioning

confidence: 99%

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

et al. 2008

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Aims/hypothesis Patients with type 1 diabetes mellitus are more susceptible than healthy individuals to exercise-induced oxidative stress and vascular endothelial dysfunction, which has important implications for the progression of disease. Thus, in the present study, we designed a randomised doubleblind, placebo-controlled trial to test the original hypothesis that oral prophylaxis with vitamin C attenuates rest and exercise-induced free radical-mediated lipid peroxidation in type 1 diabetes mellitus. Methods All data were collected from hospitalised diabetic patients. The electron paramagnetic resonance spectroscopic detection of spin-trapped α-phenyl-tert-butylnitrone (PBN) adducts was combined with the use of supporting markers of lipid peroxidation and non-enzymatic antioxidants to assess exercise-induced oxidative stress in male patients with type 1 diabetes (HbA 1c 7.9±1%, n=12) and healthy controls (HbA 1c 4.6±0.5%, n=14). Following participant randomisation using numbers in a sealed envelope, venous blood samples were obtained at rest, after a maximal exercise challenge and before and 2 h after oral ingestion of 1 g ascorbate or placebo. Participants and lead investigators were blinded to the administration of either placebo or ascorbate treatments. Primary outcome was the difference in changes in free radicals following ascorbate ingestion. Results Six diabetic patients and seven healthy control participants were randomised to each of the placebo and ascorbate groups. Diabetic patients (n=12) exhibited an elevated concentration of PBN adducts (p<0.05 vs healthy, n=14), which were confirmed as secondary, lipid-derived oxygen-centred alkoxyl (RO·) radicals (a nitrogen =1.37 mT and aβ hydrogen =0.18 mT). Lipid hydroperoxides were also selectively elevated and associated with a depression of retinol and lycopene (p<0.05 vs healthy). Vitamin C supplementation increased plasma vitamin C concentration to a similar degree in both groups (p<0.05 vs presupplementation) and attenuated the exercise-induced oxidative stress response (p<0.05 vs healthy).There were no selective treatment differences between groups in the primary outcome variable. Conclusions/interpretation These findings are the first to suggest that oral vitamin C supplementation provides an Diabetologia

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“…The degree of reticulocytosis was determined from blood films stained with Brilliant Cresyl Blue in citrate-saline solution (Dacie and Lewis, 1970).…”

Section: Preparation Of Parasitesmentioning

confidence: 99%

COMPARATIVE STUDIES ON DIHYDROOROTATE DEHYDROGENASE FROM P. BERGHEI AND THE MOUSE RETICULOCYTE

Gero

Finney

Bennett

et al. 1981

Aust J Exp Biol Med

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Summary. Kinetic parameters on dihydroorotate dehydrogenase (DHO-DHase) from the rodent malarial parasite, Plasmodium berghei, have been determined. This enzyme, the fourth in de novo pyrimidine biosynthesis, is particulate and is absent in the mature mammalian red cell. The K,,, of the substrate, dihydroorotate, was determined to be 23 MM and the Ki values for a number of substrate analogues have been determined. The most potent inhibitor was dihydroazaorotate (Kl, 3 MM). The product orotate was also a good inhibitor (Ki, 5 MM) as were methylorotate (Ki, 10 /"M), 5-azaorotate (Ki, 20 MM) and other pyrimidine analogues. The activity of the enzyme was also affected by a number of respiratory chain inhibitors.As the P. berghei infection is accompanied by reticulocytosis, a comparative study of DHO-DHase in mouse reticulocytes was also carried out. The general properties of the enzyme from these sources were similar to those of the parasite enzyme. However, significant differences in the response of the two enzymes to various inhibitors were observed and could provide a rational basis for the development of chemotherapeutic agents active against the parasite.

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Clinical Hæmatology

Cited by 134 publications

References 2 publications

Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women

Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women

Molecular detection of exercise-induced free radicals following ascorbate prophylaxis in type 1 diabetes mellitus: a randomised controlled trial

COMPARATIVE STUDIES ON DIHYDROOROTATE DEHYDROGENASE FROM P. BERGHEI AND THE MOUSE RETICULOCYTE

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