Clinical Evaluation of the New High-Throughput Luminex NxTAG Respiratory Pathogen Panel Assay for Multiplex Respiratory Pathogen Detection

Chen, Jonathan Hon-Kwan; Lam, Hoyin; Yip, Cyril Chik-Yan; Wong, Sally Cheuk-Ying; Chan, Jasper Fuk‐Woo; S.K., Edmond; Cheng, Vincent Chi-Chung; Tang, Bone Siu-Fai; Yuen, Kwok‐Yung

doi:10.1128/jcm.00517-16

Cited by 61 publications

(55 citation statements)

References 26 publications

(29 reference statements)

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“…Multiplex real‐time PCR has allowed simultaneous detection of multiple respiratory viruses in a short time. Compared with the singleplex approach, the multiplex diagnostic approach has higher sample throughput (96 samples per run), shorter turnaround time (5 hours), and a smaller amount of sample requirement 73 …”

Section: Multiplex Respiratory Virus Detectionmentioning

confidence: 99%

“…The Luminex NxTAG Respiratory Pathogen Panel (NxTAG‐RPP) is a new high‐throughput multiplex real‐time PCR system, which was upgraded from the xTAG Respiratory Viral Panel Fastv2 (RVPv2) assay 73 . This assay demonstrates good diagnostic performance on detection of multiplex respiratory pathogens.…”

Section: Multiplex Respiratory Virus Detectionmentioning

confidence: 99%

“…A total of 284 clinical respiratory specimens, including hAdV and RV, and 3 influenza A/H7N9 viral culture samples have been tested, and the overall diagnostic sensitivity of the NxTAG‐RPP was 98.9% (95% CI, 97.2 to 99.7), while the specificity was 99.0% (95% CI, 98.6 to 99.2). This assay demonstrated 100% sensitivity for detection of hAdV 73 . A number of such assays have been developed, as described, and are summarized in Table 2.…”

Section: Multiplex Respiratory Virus Detectionmentioning

confidence: 99%

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Recent advances in the detection of respiratory virus infection in humans

Zhang

Wang

Deng

et al. 2020

Journal of Medical Virology

404

363

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Respiratory tract viral infection caused by viruses or bacteria isone of the most common diseases in human worldwide, while those caused by emerging viruses, such as the novel coronavirus, 2019-nCoV that caused the pneumonia outbreak in Wuhan, China most recently, have posed great threats to global public health. Identification of the causative viral pathogens of respiratory tract viral infections is important to select an appropriate treatment, save people's lives, stop the epidemics, and avoid unnecessary use of antibiotics. Conventional diagnostic tests, such as the assays for rapid detection of antiviral antibodies or viral antigens, are widely used in many clinical laboratories. With the development of modern technologies, new diagnostic strategies, including multiplex nucleic acid amplification and microarray-based assays, are emerging. This review summarizes currently available and novel emerging diagnostic methods for the detection of common respiratory viruses, such as influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus, and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. It is anticipated that such data will assist researchers and clinicians to develop appropriate diagnostic strategies for timely and effective detection of respiratory virus infections. K E Y W O R D S adenovirus, coronavirus, diagnostic methods, influenza virus, respiratory syncytial virus, respiratory viral infection, rhinovirus

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Section: Multiplex Respiratory Virus Detectionmentioning

confidence: 99%

Section: Multiplex Respiratory Virus Detectionmentioning

confidence: 99%

Section: Multiplex Respiratory Virus Detectionmentioning

confidence: 99%

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Recent advances in the detection of respiratory virus infection in humans

Zhang

Wang

Deng

et al. 2020

Journal of Medical Virology

404

363

View full text Add to dashboard Cite

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“…A recent evaluation of NxTAG RPP RUO against a composite standard of BioFire FilmArray respiratory panel or singleplex realtime PCR by Chen et al reported Ն96% sensitivity and specificity rates for all respiratory targets except CoV-OC43 (19). Similarly, Beckmann et al compared NxTAG RPP to a reference multiplex nucleic acid amplification testing (RespiFinder-22) and also de-termined high detection rates of Ͼ94.7% for all targets except for CoV-OC43 (88.9%), CoV-NL63 (83.3), and BoV (90%) (16).…”

Section: Discussionmentioning

confidence: 99%

Comparison of NxTAG Respiratory Pathogen Panel and Anyplex II RV16 Tests for Multiplex Detection of Respiratory Pathogens in Hospitalized Children

et al. 2016

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Childhood acute lower respiratory infections (ALRI) remain as the most important single cause of global burden of disease among pediatric populations and a major cause of child mortality (1-3). It has been estimated that approximately 165 million episodes of ALRI occur globally each year in children aged 0 to 4 years, resulting in 2.1 million deaths (4). Management of these infections requires extensive use of health care and economic resources: viral ALRI alone has been described to account for an expenditure of approximately 2.4 billion dollars annually (3). Rapid etiological diagnosis of ALRI, followed by timely and specific treatment, has the potential to improve clinical outcomes and reduce the duration of hospital stay, as well as reduce unnecessary laboratory testing and antibiotic use (5, 6). However, discerning the causal agent of the infection is challenging in younger children, who typically present with nonspecific clinical symptoms (7,8). Frequent occurrence of mixed viral and bacterial-viral infections at early ages (9, 10) makes etiological diagnosis even more difficult.Nucleic acid amplification techniques are widely acknowledged to contribute improved sensitivity and rapidness over traditional methods such as culture and direct fluorescent antibody detection for diagnosing ALRI (11,12). In recent years, the introduction of novel molecular assays that allow the performance of multiple analyses in one reaction has widened the spectrum of detectable respiratory pathogens while increasing throughput and convenience (13)(14)(15). The NxTAG respiratory pathogen panel (RPP) RUO (Luminex Molecular Diagnostics, Toronto, Ontario, Canada) is a qualitative reverse transcriptase PCR assay capable of detecting and differentiating the following 22 targets in a single patient sample: adenovirus (ADV), bocavirus (BoV), coronavirus (CoV) types 229E/NL63/OC43/HKU1, influenza A virus (IFV-A; including differentiating subtypes H1/H1N1/H3), influenza B virus (IFV-B), metapneumovirus (MPV), parainfluenza virus (PIV) types 1/2/3/4, respiratory syncytial virus (RSV) types A/B, enterovirus/rhinovirus (EV/RV), Chlamydophila pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila (16). Similarly, Anyplex II RV16 (Seegene, Inc., Seoul, South Korea) is a qualitative multiplex PCR able to detect 16 targets, including AdV, BoV CoV types 229E/NL63/OC43, EV, IFV-A, IFV-B, MPV, PIV types 1 to 4, RSV types A/B, and RV (17). Both NxTAG RPP and Anyplex II RV16 RUO are Conformité Européene (CE)-marked assays. However, while the former has obtained U.S. Food and Drug Administration clearance (except for the Legionella target), the latter is not available in the United States.The primary objective of this study was to evaluate the diagnostic accuracy of NxTAG RPP RUO in comparison to Anyplex II RV16 using respiratory specimens prospectively collected from pediatric patients hospitalized at a referral medical center out of MATERIALS AND METHODS Study design and setting.A cross-sectional study was conducted to analyze nasopharyng...

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“…The potential for multiplexing of this technology is immense and has been used for simultaneous identification and/ or genotyping of microbes. [23][24][25] Any discussion regarding advances in clinical microbiology would be incomplete without mention of the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). Few technologic advances have made as much impact on the identification of microbes.…”

Section: Microarraysmentioning

confidence: 99%

The new frontier of diagnostics: Molecular assays and their role in infection prevention and control

Das

Shibib

Vernon

2017

American Journal of Infection Control

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Recent advances in technology over the last decade have propelled the microbiology laboratory into a pivotal role in infection prevention and control. The rapid adaptation of molecular technologies to the field of clinical microbiology now greatly influences infectious disease management and significantly impacts infection control practices. This review discusses recent developments in molecular techniques in the diagnosis of infectious diseases. It describes the basic concepts of molecular assays, discusses their advantages and limitations, and characterizes currently available commercial assays with respect to cost, interpretive requirements, and clinical utility.

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Clinical Evaluation of the New High-Throughput Luminex NxTAG Respiratory Pathogen Panel Assay for Multiplex Respiratory Pathogen Detection

Cited by 61 publications

References 26 publications

Recent advances in the detection of respiratory virus infection in humans

Recent advances in the detection of respiratory virus infection in humans

Comparison of NxTAG Respiratory Pathogen Panel and Anyplex II RV16 Tests for Multiplex Detection of Respiratory Pathogens in Hospitalized Children

The new frontier of diagnostics: Molecular assays and their role in infection prevention and control

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