Clinical Evaluation of the Combination of Anorganic Bovine‐Derived Hydroxyapatite Matrix/Cell‐Binding Peptide (P‐15) in Particulate and Hydrogel Form as a Bone Replacement Graft Material in Human Periodontal Osseous Defects: 6‐Month Reentry Controlled Clinical Study

Matos, Sérgio; Guerra, Fernando; Krauser, Jack T.; Marques, Francisco Batel; Ermida, Jorge; Sanz, Mariano

doi:10.1902/jop.2007.060474

Cited by 23 publications

(25 citation statements)

References 30 publications

(50 reference statements)

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“…CMC and HPMC are considered suitable carriers based on evidence that both substances support the osteoinductive capacities of BMP‐25, 21–27 and BMP‐7,6–10, 28 as well as the osteoconductive properties of bone substitutes 29. However, whether CMC or HPMC can control the formation or the activity of osteoclasts and osteoblasts is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Preclinical models and clinical studies suggest that CMC is a suitable carrier for recombinant growth factors with osteoinductive properties, for example, bone morphogenetic protein (BMP)‐21–4 and osteogenic protein‐1 (OP‐1/BMP‐7) 5. CMC also serves as a carrier for bone substitutes such as calcium sulfate6 and a modified bovine‐derived hydroxyapatite matrix 7, 8. HPMC and chemical modifications thereof have been used as carriers for bone substitutes9 and bone cements 10.…”

Section: Introductionmentioning

confidence: 99%

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Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

Agis

Beirer

Watzek

et al. 2010

J Biomedical Materials Res

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Carboxymethylcellulose (CMC) and hydroxypropylmethylcellulose (HPMC) serve as carriers for growth factors and bone substitutes. Although both carriers are placed into the defect sites, their impacts on bone regeneration are unclear. Herein, we examined whether CMC and HPMC affect the differentiation of bone marrow progenitors into osteoclasts and osteoblasts. We therefore induced osteoclastogenesis and osteoblastogenesis in murine bone marrow progenitors in the presence of CMC and HPMC, respectively. Measures of osteoclastogesis were based on the number and activity of tartrate-resistant acid-phosphatase-positive (TRAP(+)) multinucleated cells and expression of marker genes. Osteoblastogenesis was determined by the number and activity of alkaline-phosphatase-positive (AP(+)) colonies and relevant marker genes. Viability was assessed by colorimetric measurement of formazan formation. We report that CMC at 1% caused a significant reduction in the number and activity of TRAP(+) multinucleated cells. Changes in viability were not responsible for the observed effects. HPMC showed no remarkable impact on osteoclastogenesis; however, the concentration was limited to 0.5% because of the high viscosity. The ability of bone marrow progenitors to form AP(+) colonies was not affected by either of the two carriers. Together, these results suggest that CMC and possibly also HPMC can decrease osteoclastogenesis while osteoblastogenesis remains unchanged in vitro. These observations raise the possibility that these carriers might affect the cellular process of bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

Agis

Beirer

Watzek

et al. 2010

J Biomedical Materials Res

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“…Only one case report shows that use of ABM/P-15 can result in periodontal regeneration on previously diseased root surfaces [252]. To develop an injectable biomimetic matrix to facilitate clinical application, ABM/P-15 can be suspended in injectable hyaluronate (Hy) hydrogels [253,254]. There is more and more evidence that suggests P-15 cell-binding peptide, when complexed with ABM, promotes cell survival [255] and modulates proliferation and mRNA synthesis of GFs of PDL cells [256].…”

Section: Cell Binding Peptide (P-15)mentioning

confidence: 99%

A review on endogenous regenerative technology in periodontal regenerative medicine

et al. 2010

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“…In some studies that treated infra-osseous defects with absorbable barriers 8,18 or other regenerative therapies 20 , surgical re-entry was complemented by the radiographic subtraction technique to evaluate bone density gain.…”

Section: Discussionmentioning

confidence: 99%

“…The properties of osteoconduction and integration into bone tissue 26 have enabled the use of xenogenous grafts derived from bovine bone (BDX) in regenerative periodontal therapy 11,20,24,30 . The material is slowly reabsorbed by osteoclastic activity 2 .…”

Section: Introductionmentioning

confidence: 99%

Digital subtraction radiographic analysis of the combination of bioabsorbable membrane and bovine morphogenetic protein pool in human periodontal infrabony defects

et al. 2010

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ObjectivesThis study assessed the bone density gain and its relationship with the periodontal clinical parameters in a case series of a regenerative therapy procedure.Material and MethodsUsing a split-mouth study design, 10 pairs of infrabony defects from 15 patients were treated with a pool of bovine bone morphogenetic proteins associated with collagen membrane (test sites) or collagen membrane only (control sites). The periodontal healing was clinically and radiographically monitored for six months. Standardized presurgical and 6-month postoperative radiographs were digitized for digital subtraction analysis, which showed relative bone density gain in both groups of 0.034 ± 0.423 and 0.105 ± 0.423 in the test and control group, respectively (p>0.05).ResultsAs regards the area size of bone density change, the influence of the therapy was detected in 2.5 mm2 in the test group and 2 mm2 in the control group (p>0.05). Additionally, no correlation was observed between the favorable clinical results and the bone density gain measured by digital subtraction radiography (p>0.05).ConclusionsThe findings of this study suggest that the clinical benefit of the regenerative therapy observed did not come with significant bone density gains. Long-term evaluation may lead to a different conclusions.

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Clinical Evaluation of the Combination of Anorganic Bovine‐Derived Hydroxyapatite Matrix/Cell‐Binding Peptide (P‐15) in Particulate and Hydrogel Form as a Bone Replacement Graft Material in Human Periodontal Osseous Defects: 6‐Month Reentry Controlled Clinical Study

Abstract: This trial failed to demonstrate superiority of the novel ABM/P-15 hydrogel therapeutic modality over the standard ABM/ P-15 particulate graft in the treatment of intrabony periodontal defects.

Cited by 23 publications

References 30 publications

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

Effects of carboxymethylcellulose and hydroxypropylmethylcellulose on the differentiation and activity of osteoclasts and osteoblasts

A review on endogenous regenerative technology in periodontal regenerative medicine

Digital subtraction radiographic analysis of the combination of bioabsorbable membrane and bovine morphogenetic protein pool in human periodontal infrabony defects

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