Clinical Evaluation of a Biodegradable Collagen Membrane in Guided Tissue Regeneration

Abstract

A bio-resorbable type I collagen membrane was investigated as a barrier for guided tissue regeneration. Ten human subjects with at least one pair of contralateral periodontal lesions with probing pocket depths of greater than or equal to 5 mm and radiographic evidence of greater than or equal to 40% bone loss were included. Each patient underwent contralateral surgical flap procedures. A collagen barrier was adapted to the tooth in the experimental defect and the flap replaced and sutured. The controls consisted of the same procedure without the placement of the barrier. Standardized measurements of change in probing attachment levels and fill of intrabony defects were obtained at the time of surgery and 1 year later at the time of surgical re-entry. The differences in change of probing attachment levels and amount of bone fill between individual test and control sites were compared utilizing the student's t-test for paired samples. The mean probing attachment gain in the test sites was 0.56 +/- 0.57 mm, and there was a mean probing attachment loss of 0.71 +/- 0.91 mm in the control sites (P less than 0.01). The gain of bone in test lesions was 1.16 +/- 0.95 mm, while no gain was observed in the control lesions (P less than 0.01). The results of this study demonstrated that sites treated with a collagen barrier comprised of cross-linked bovine Type I collagen exhibited significantly better healing as compared to control sites over the 1-year period of the study.

Morphologic study and syntheses of type I collagen and fibronectin of human periodontal ligament cells cultured on poly(ethylene-co-vinyl alcohol) (EVA) with collagen immobilization

“…[1][2][3] A variety of applications of collagen has been reported to some diseases involving wound dressing, 4 peripheral nerve growth support, 5 and guided tissue regeneration for periodontal diseases. 6 We have attempted to regenerate the periodontal ligament (PDL) around dental titanium implants through PDL cell culture on collagen-immobilized implant [titanium + poly(ethylene-co-vinyl alcohol) (EVA) + collagen]. EVA has a high affinity with titanium and is so bioinert as to be applied to blood materials such as filter and permeability membranes.…”

Implications of cellular and molecular biology advances in periodontal regeneration

“…Several studies (Pitaru, 1987(Pitaru, , 1989YafTe, 1987) reported utilizing various forms of type I collagen as a membrane barrier for guided tissue regeneration procedures. Clinical human studies (Chung et al, 1990;Anderson, 1991) have also demonstrated positive results with type I collagen membranes in the treatment of periodontal defects using guided tissue regeneration techniques. Mendieta and Williams (1994) highlighted in their review the potential of crosslinked type I collagen together with polylactic acid as a suitable absorbable membrane.…”

Physiochemical properties and resorption progress of porcine skin-derived collagen membranes: <i>In vitro</i> and <i>in vivo</i> analysis

“…Studies investigating commonly used techniques for tissue regeneration, including guided bone regeneration (GBR) and guided tissue regeneration (GTR), have emphasized the need for barrier membranes to prevent the apical growth of unwanted cells (epithelium and gingival connective tissue) within the wound area and maintain space for an adequate period during the early healing phase [3][4][5] .…”