Clinical decision making in Barrett's oesophagus can be supported by computerized immunoquantitation and morphometry of features associated with proliferation and differentiation

Grading of dysplasia in Barrett's oesophagus: substantial interobserver variation between general and gastrointestinal pathologists

“…20 Montgomery et al 13 systematically investigated the effect of a consensus meeting, which, it was shown, brought hardly any improvement in interobserver variability. A strategy to overcome difficulties with subjective grading involves the use of morphometry, as has been performed by Polkowski et al 21 However, this approach requires dedicated equipment and expertise, which is not universally available. Furthermore, morphometry provides no solutions to such technical issues as tangential cutting or severe inflammation.…”

JARID1B Protein Expression and Prognostic Implications in Uveal Melanoma

“…A modified quantitative adaptation of stereology [37][38][39] was used to evaluate the IHC results. This method yielded approximate data by estimating the percentage of immunoreactive cells irrespective of staining intensity and assessing fields of vision covering the entire tumor area.…”

Advances in cancer tissue microarray technology: Towards improved understanding and diagnostics

“…There now also exist multiple commercial products which are aimed at quantitative IHC analysis such as the ChromaVision Automated Cellular Imaging System (ChromaVision Medical Systems Inc., San Juan Capistrano, CA) [112], the Image Tiler (Tripath Imaging, Burlington, NC) [113], and QPRODIT (Leica Imaging Systems Ltd., Cambridge, UK) [109], TMAscore (Bacus Instruments, Lombard, IL), and TMAx (Breecher Instruments, Sun Prairie, WI). Most of these approaches rely upon manual delineation of the region of interest (ROI) before analysis can begin [109,114].…”