Clinical chemistry reference database for Wistar rats and C57/BL6 mice

Boehm, Olaf; Zur, Berndt; Koch, Alexander; Tran, Nguyen; Freyenhagen, Rainer; Hartmann, Matthias; Zacharowski, Kai

doi:10.1515/bc.2007.061

Cited by 126 publications

(91 citation statements)

References 18 publications

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“…Serum cholesterol concentration in rats that were fed the cholesterol-enriched control diet (3.41 µmol ml -1 ) exceeded the median value of the cholesterol concentration reported for female Wistar rats (1.50 µmol ml -1 ; [16]). In two previous experiments [8] with male rats fed an almost identical diet containing cholesterol at 10 g kg -1 , the serum cholesterol concentration was 1.99 and 2.08 µmol ml -1 .…”

Section: Discussionmentioning

confidence: 52%

Comparative effect of amidated pectin and psyllium on cholesterol homeostasis in rats

et al. 2010

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Abstract:The effects of amidated pectin and psyllium on serum, hepatic and faecal cholesterol concentration were compared in female rats fed diets supplemented with palm fat and cholesterol at 50 and 10 g/kg, respectively. Control rats were fed a diet supplemented with cellulose at 60 g/kg. In treated rats, cellulose was replaced with either amidated pectin or psyllium. Amidated pectin and psyllium intake significantly decreased serum cholesterol from 3.41 µmol/ml (control) to 1.68 and 2.04 µmol/ml, respectively, and hepatic cholesterol from 31.9 µmol/g (control) to 7.2 and 9.0 µmol/g, respectively. Histology with lipid-staining Sudan Black B revealed that liver tissue from control rats was infiltrated with lipids, but staining was absent in livers of treated rats. No hepatic pathophysiology was apparent in treated rats. Amidated pectin and psyllium intake significantly increased faecal fat content. Faecal cholesterol content was significantly increased in rats that were fed amidated pectin, and non-significantly increased in rats that were fed psyllium. Body weight and food intake did not differ among treatment groups. In conclusion, amidated pectin, a novel sequestrant of sterols, demonstrated a similar effect on rat serum and hepatic cholesterol concentration to psyllium, which is a well-established hypocholesterolaemic agent.© Versita Sp. z o.o.

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Section: Discussionmentioning

confidence: 52%

Comparative effect of amidated pectin and psyllium on cholesterol homeostasis in rats

et al. 2010

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“…Sporadic differences in some analytes were not treatment related and did not affect either gross or microscopic pathology and therefore were interpreted as biological variability normally observed in Wistar rats. 17,20 Treatment-related morphological abnormalities were not found in any organs/tissues examined. These findings confirm other studies showing that, as for AFA, high exposure to StemEnhance does not lead to toxicity.…”

Section: Resultsmentioning

confidence: 88%

Subacute toxicity study in Wistar rats fed with StemEnhanceTM, an extract from Aphanizomenon flos-aquae

Dirikolu¹,

Chakkath²,

Ball-Kell³

et al. 2010

NDS

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This study evaluated the safety (absence of toxicity) of StemEnhance™, an extract of the blue-green alga Aphanizomenon flos-aquae that is used as a health supplement. Groups of 12 rats of each sex were given either 5% glycerin in water (control) or 600 mg/kg of StemEnhance prepared in 5% glycerin in water for 2 weeks by oral gavage followed by 2 weeks of observation. The administration of StemEnhance had no effect on behavior, food and water intake, growth, or survival. Values at the end of dosing and observation periods did not reveal differences between treated and control groups for hematology and clinical chemistry. There were no significant differences in the gross and histopathology of the reproductive organs in either males or females. Sperm motility parameters were similar for control and treated males. Our results show that StemEnhance at doses ∼20 times the maximum label-recommended daily dose did not produce adverse effects in Wistar rats after subacute treatment.

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“…Tabla I. Concentraciones plasmáticas de lípidos antes y después del consumo de la dieta hipercolesterolémica D12336 en ratones C57BL/6. *Test t de Student; ** Boehm et al (2007).…”

Section: Resultsunclassified

“…Concentraciones plasmáticas de lípidos en ratones C57BL/6 que consumieron dieta D12336 y después del tratamiento con ezetimiba (5mg/kg/día/1mes). *Test t de Student; ** Boehm et al (2007).…”

Section: Resultsunclassified

La Respuesta Terapéutica a Ezetimiba en Ratones C57BL/6 es Mediada por Cambios en la Expresión de NPC1L1, ABCG5 y ABCG8 en el Enterocito

Caamaño¹,

Saavedra

Wulff

et al. 2012

Int. J. Morphol.

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RESUMEN:Las proteínas NPC1L1, ABCG5 y ABCG8 participan en la absorción intestinal de colesterol. Ezetimiba inhibe este proceso bloqueando a NPC1L1, sin embargo, su efecto sobre ABCG5 y ABCG8 aún no está claro. Así, el objetivo del presente trabajo fue evaluar en ratones C57BL/6 con hipercolesterolemia inducida por dieta y tratados con ezetimiba, la expresión de NPC1L1, ABCG5 y ABCG8 mediante PCR en tiempo real y Western blot, en 3 grupos de animales: 1, dieta hipercolesterolémica D12336; 2, dieta D12336 más 5 mg/kg/día de ezetimiba; 3, dieta control. El nivel sérico de colesterol total fue significativamente diferente entre los grupos estudiados (control: 1,85 ± 0,49 mmol/L; dieta D12336: 3,11 ± 0,73 mmol/L; ezetimiba: 2,11 ± 0,50 mmol/L, P = 0,001). La expresión génica de NPC1L1 aumentó 5,4 veces en el grupo que recibió la dieta D12336 (P = 0,003). Por otro lado, la expresión génica de ABCG5 y ABCG8 no fue diferente en el grupo con hipercolesterolemia (P = 0,239 y P = 0,201, respectivamente). Después del tratamiento con ezetimiba, la expresión génica de ABCG5 se incrementó 15,6 veces (P = 0.038). No hubo diferencias significativas en la expresión génica de NPC1L1 (P = 0,134) y ABCG8 (P = 0,067). En relación a la expresión proteica, la dieta D12336 incrementó los niveles de expresión de NPC1L1 (P = 0,022) y ABCG5 (P = 0,008); el tratamiento con ezetimiba incrementó los niveles de NPC1L1 (P = 0,048) y redujo los niveles de ABCG5 (P = 0,036) y ABCG8 (P = 0,016). En conclusión, nuestros resultados sugieren que tanto la dieta hipercolesterolémica como el tratamiento con ezetimiba, en un modelo experimental, afectan los niveles de expresión de NPC1L1, ABCG5 y ABCG8, sugiriendo que ABCG5 y ABCG8 están involucrados en la respuesta hipolipemiante a este fármaco. No obstante, el mecanismo mediante el cual se explica esta interacción requiere de un futuro estudio.PALABRAS CLAVE: Ezetimiba; Colesterol; NPC1L1; ABCG5/G8. INTRODUCCIÓNSe ha establecido que la captación de colesterol por los enterocitos se debe a mecanismos facilitados por proteí-nas (Lammert & Wang, 2005). En el año 2004, la proteína Niemann-Pick C1-like 1 (NPC1L1), fue identificada como responsable de la absorción de colesterol de la dieta a nivel intestinal (Altmann et al., 2004). Además, la cantidad de colesterol absorbido puede ser regulada a través de transportadores ATP-binding cassette (ABC), localizados en la membrana del enterocito, que ejercen su acción limitadora de absorción de colesterol mediante la secreción de vuelta al lumen intestinal del colesterol internalizado (Hui & Howles, 2005). ABCG5 y ABCG8, forman un heterodímero que está involucrado en el eflujo de esteroles de la dieta desde las células epiteliales intestinales hacia el lumen del intestino y del hígado al ducto biliar (Schmitz et al., 2001). Experimentos desarrollados en ratones knock-out para ABCG5/ABCG8 han permitido confirmar que ABCG5 y ABCG8 son los mayores transportadores hepatobiliares de colesterol, que protegen frente a la acumulación de esterol dietético en el cuerpo y qu...

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Clinical chemistry reference database for Wistar rats and C57/BL6 mice

Cited by 126 publications

References 18 publications

Comparative effect of amidated pectin and psyllium on cholesterol homeostasis in rats

Comparative effect of amidated pectin and psyllium on cholesterol homeostasis in rats

Subacute toxicity study in Wistar rats fed with StemEnhanceTM, an extract from Aphanizomenon flos-aquae

La Respuesta Terapéutica a Ezetimiba en Ratones C57BL/6 es Mediada por Cambios en la Expresión de NPC1L1, ABCG5 y ABCG8 en el Enterocito

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