Clinical, biophysical and immunohistochemical analysis of skin reactions to acute skin barrier disruption - a comparative trial between participants with sensitive skin and those with nonsensitive skin

Richters, R.J.H.; Uzunbajakava, N.; Falcone, Denise; Hendriks, Jan C.M.; Jaspers, Eva; Kerkhof, P.C.M. van de; Erp, P.E.J. van

doi:10.1111/bjd.14307

Cited by 14 publications

(40 citation statements)

References 30 publications

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“…We found, however, significantly less mast cells in SS subjects compared to NSS subjects at 24 h after stimulus (À19.5, 95% CI: (À36.7;À2.4) (P = 0.029)), (Figs 1 and 2). This finding is in line with Richters et al studying skin responses to mechanical barrier disruption, in which a trend in a lower number of mast cells following stimulus in SS subjects was observed (10).…”

Section: Resultssupporting

confidence: 92%

“…These results confirm that the SC is vulnerable to surfactants, especially in SS. Other studies show that the SC is also vulnerable to mechanical stimulation, therefore suggesting that SS might be triggered by factors of different nature (10). As TEWL measurements were not able to discriminate between SS and NSS, it could mean that the SC structure might be malformed and establish a slightly impaired barrier at a level that is beyond sensitivity of traditional biophysical methods.…”

Section: Resultsmentioning

confidence: 98%

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Responses to sodium dodecyl sulphate as an in vivo human model to study the pathomechanisms underlying sensitive skin

Richters

Hendriks²,

Uzunbajakava

et al. 2016

Experimental Dermatology

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 98%

Responses to sodium dodecyl sulphate as an in vivo human model to study the pathomechanisms underlying sensitive skin

Richters

Hendriks²,

Uzunbajakava

et al. 2016

Experimental Dermatology

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“…Potential participants were recruited via websites and asked to fill the novel perception-based questionnaire [17], as previously described [18,19,20]. Briefly, inclusion was based on self-assessed skin sensitivity and on a sensitive skin score obtained by rating skin perceptions (i.e., discomfort, stinging, redness, dryness) following exogenous and endogenous triggers (i.e., toiletries, shaving, heat, cold, clothes, emotions).…”

Section: Methodsmentioning

confidence: 99%

“…With as much as 50-61% women and 30-44% men reporting SS in the industrialized world, and consensus on the definition and pathomechanism remaining elusive despite extensive research [15], SS has been identified as a global challenge [16]. However, recent clinical studies, based on the selection of a SS and NSS panel according to a novel perception-based questionnaire [17] and on the application of in vivo skin models followed by clinical, biophysical, and immunohistochemical analysis, have shown that aberrant vascular and inflammatory reactions, accompanied by enhanced sensory reactivity, were the hallmarks of SS [18,19,20]. We thus hypothesized that the triple response elicited by histamine iontophoresis might differ between SS and NSS subjects.…”

Section: Introductionmentioning

confidence: 99%

Histamine Iontophoresis as in vivo Model to Study Human Skin Inflammation with Minimal Barrier Impairment: Pilot Study Results of Application of the Model to a Sensitive Skin Panel

Falcone

Uzunbajakava²,

Richters³

et al. 2017

Skin Pharmacol Physiol

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Background/Aims: Histamine iontophoresis is known to elicit itch and a wheal-and-flare reaction; however, its impact on the skin barrier and underlying compartments has not been thoroughly evaluated yet. The primary objective of this study was to characterize that using immunohistochemistry, biophysical measurements, and image analysis, and secondly, to explore whether skin reactions to this model differ in sensitive skin (SS). Methods: Eighteen healthy subjects, n = 9 with SS and n = 9 with non-sensitive skin (NSS), were included based on a perception-based questionnaire. Histamine iontophoresis was performed on the buttock, and skin reactions were evaluated up to 72 h after stimulation. Results: The wheal-and-flare peaked at 30 min; after 8 h, no clinical signs were visible. No signs of disruption of the stratum corneum, as well as no increase in the number of Ki67-positive cells emerged, whereas fewer tryptase-positive mast cells and increased epidermal thickness were observed at 1 and 72 h, respectively. SS subjects showed higher perception of itch compared to NSS subjects. Conclusion: Histamine iontophoresis is a well-standardized in vivo model to quantitatively study the early stages of cutaneous inflammation with minimal impact on the skin barrier. In line with previous studies, it highlighted increased sensory perceptions in SS.

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“…Skin barrier function is affected in patients with extensive list of dermatological diseases including lamellar ichthyosis, psoriasis, Netherton syndrome, Chanarin-Dorfman and atopic dermatitis (AD) [10] and has been suggested to play a role in sensitive skin [11][12][13].…”

Section: Importance Of Skin Barrier Lipids and Water For Cosmetic-anmentioning

confidence: 99%

Potential of short-wave infrared spectroscopy for quantitative depth profiling of stratum corneum lipids and water in dermatology

Ezerskaia¹,

Uzunbajakava²,

Puppels³

et al. 2018

Biomed. Opt. Express

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Abstract:We demonstrate the feasibility of short wave infrared (SWIR) spectroscopy combined with tape stripping for depth profiling of lipids and water in the stratum corneum of human skin. The proposed spectroscopic technique relies on differential detection at three wavelengths of 1720, 1750, and 1770 nm, with varying ratio of the lipid-to-water absorption coefficient and an 'isosbestic point'. Comparison of the data acquired using SWIR spectroscopy with that obtained by a gold standard for non-invasive quantitative molecularspecific skin measurements, namely confocal Raman spectroscopy (CRS), revealed specificity of the proposed modality for water and lipid quantification. At the same time, we provide evidence showing aberrant sensitivity of Corneometer hydration read-outs to the presence of skin surface lipids, and a lack of sensitivity of the Sebumeter when attempting to measure the lipids of the cornified lipid envelope and intracellular lipid layers. We conclude that a spectroscopic SWIR-based spectroscopic method combined with tape stripping has the potential for depth profiling of the stratum corneum water and lipids, due to superior measurement sensitivity and specificity compared to the Corneometer and Sebumeter. References and links1. D. J. Tobin, "Introduction to skin aging," J. Tissue Viability 26(1), 37-46 (2017). 2. P. C. Arck, A. Slominski, T. C. Theoharides, E. M. Peters, and R. Paus, "Neuroimmunology of stress: skin takes center stage," J. Invest. Dermatol. 126(8), 1697-1704 (2006). 3. K. R. Feingold and P. M. Elias, "Role of lipids in the formation and maintenance of the cutaneous permeability barrier," Biochim. Biophys. Acta 1841(3), 280-294 (2014). 4. D. J. Tobin, "Biochemistry of human skin--our brain on the outside," Chem. Soc. Rev. 35(1), 52-67 (2006). 5. M. Akiyama, "Corneocyte lipid envelope (CLE), the key structure for skin barrier function and ichthyosis pathogenesis," J. Dermatol. Sci. 88(1), 3-9 (2017). 6. K. C. Madison, "Barrier function of the skin: "la raison d'être" of the epidermis," J. Invest. Dermatol. 121(2), 231-241 (2003). 7. D. J. Tobin, "Introduction to skin aging," J. Tissue Viability 26(1), 37-46 (2017 75-83 (2015). 13. R. J. Richters, N. E. Uzunbajakava, D. Falcone, J. C. Hendriks, E. J. Jaspers, P. C. van de Kerkhof, and P. E. van Erp, "Clinical, biophysical and immunohistochemical analysis of skin reactions to acute skin barrier disruptiona comparative trial between participants with sensitive skin and those with nonsensitive skin," Br. J. Dermatol. 174(5), 1126-1133 (2016). 14. M. Akiyama, "Corneocyte lipid envelope (CLE), the key structure for skin barrier function and ichthyosis pathogenesis," J. Dermatol. Sci. 88(1), 3-9 (2017). 15. M. Sullivan, N.B. Silverberg, "Current and emerging concepts in atopic dermatitis pathogenesis," Clin, Dermatol.35 (

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Clinical, biophysical and immunohistochemical analysis of skin reactions to acute skin barrier disruption - a comparative trial between participants with sensitive skin and those with nonsensitive skin

Cited by 14 publications

References 30 publications

Responses to sodium dodecyl sulphate as an in vivo human model to study the pathomechanisms underlying sensitive skin

Responses to sodium dodecyl sulphate as an in vivo human model to study the pathomechanisms underlying sensitive skin

Histamine Iontophoresis as in vivo Model to Study Human Skin Inflammation with Minimal Barrier Impairment: Pilot Study Results of Application of the Model to a Sensitive Skin Panel

Potential of short-wave infrared spectroscopy for quantitative depth profiling of stratum corneum lipids and water in dermatology

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