Clinical applications of chromosome analysis, from fine needle aspiration biopsies, of posterior uveal melanomas

Sisley, Karen; Nichols, C; Parsons, M A; Farr, R. Wesley; Rees, RC; Rennie, IG

doi:10.1038/eye.1998.48

Cited by 33 publications

(24 citation statements)

References 17 publications

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“…These authors concluded that FNAB of uveal melanoma provided sufficient samples and was reliable for FISH analysis for chromosome 3 or 8 abnormalities. Sisley et al 17 found similar accurate correlation of cytogenetic analysis in FNAB compared with solid tumor samples after enucleation in 10 cases.…”

Section: Commentsupporting

confidence: 51%

Chromosome 3 Analysis of Uveal Melanoma Using Fine-Needle Aspiration Biopsy at the Time of Plaque Radiotherapy in 140 Consecutive Cases

Shields¹

2007

Arch Ophthalmol

118

113

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To evaluate the feasibility of genetic testing of uveal melanoma using fine-needle aspiration biopsy (FNAB).Methods: We reviewed the clinical records of all patients of the Ocular Oncology Service at Wills Eye Hospital with the diagnosis of uveal melanoma who underwent FNAB for genetic testing for chromosome 3 status between November 1, 2005, and March 1, 2006. The FNAB was performed immediately before plaque radiotherapy. The specimens underwent genetic analysis using DNA amplification and microsatellite assay to determine the presence of monosomy 3.Results: A total of 140 eyes of 140 patients with uveal melanoma were sampled for chromosome 3 abnormalities using FNAB. Monosomy 3 was found in 44 cases (31%), disomy 3 was found in 76 cases (54%), and the genomic DNA yield was insufficient for genetic analysis in 20 cases (14%). Monosomy 3 was found in 16 of 61 small melanomas (26%), 24 of 67 medium melanomas (36%), and 4 of 12 large melanomas (33%). Adequate DNA was achieved in 97% of cases using a 27-gauge needle via the transvitreal tumor apex approach and in 75% of cases using a 30-gauge needle via the transscleral tumor base approach. Factors predictive of monosomy 3 included greater tumor basal dimension (P=.02) and greater distance from the optic disc (P = .02). Transient localized vitreous hemorrhage was found in 46% of eyes. No cases of diffuse vitreous hemorrhage, retinal detachment, or tumor recurrence along the biopsy tract were found. Conclusion:We found that in most cases, FNAB provides adequate DNA for genetic analysis of uveal melanoma using microsatellite assay.

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Section: Commentsupporting

confidence: 51%

Chromosome 3 Analysis of Uveal Melanoma Using Fine-Needle Aspiration Biopsy at the Time of Plaque Radiotherapy in 140 Consecutive Cases

Shields¹

2007

Arch Ophthalmol

118

113

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“…Therefore, the application of FISH to FNAB is considered a reliable method for detecting genetic prognostic parameters, and cytologic material obtained using FNAB seems highly representative of the tumor's genetic characteristics [2,17]. We considered that moving detection of monosomy 3 from the ex vivo to the in vivo arena may represent a relevant improvement in the clinical identification of this risk factor in patients affected by posterior uveal melanoma.…”

Section: Discussionmentioning

confidence: 99%

“…FNAB has already been used to sample enucleated eyes to investigate cytogenetic abnormalities with promising results [2,17]. Therefore, the application of FISH to FNAB is considered a reliable method for detecting genetic prognostic parameters, and cytologic material obtained using FNAB seems highly representative of the tumor's genetic characteristics [2,17].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, monosomy 3 is considered a highly specific marker for poor prognosis in posterior uveal melanoma [6,8,[14][15][16]. Currently, cytogenetic studies on posterior uveal melanomas are performed only on biologic material taken from enucleated eyes or histologic specimens from resected tumors [16,17]. Moreover, accurate identification of patients at high risk of developing metastatic disease may be relevant for future clinical management, but patients treated with radiation seem to be excluded from any prognostic information, except clinical data [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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In vivo monosomy 3 detection of posterior uveal melanoma: 3-year follow-up

Midena

Bonaldi

Parrozzani

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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“…Starting a little over a decade ago, investigators around the world began to report that chromosomal and transcriptional features of primary uveal melanoma cells were strongly associated with the pa tient's likelihood of developing subsequent extraophthalmic metastasis and metastatic death (25,(29)(30)(31)(32)(33)(34)(35) Several authors have shown that these chromosomal and transcriptional features can be determined on tumor specimens obtained by FNAB changing the indication for FNAB from purely diagnostic to investigational-prognostic (24,25,(36)(37)(38) . During the past few years, investigators from several of these centers have reported their experience with cytogenetic testing of the obtained FNAB aspirates (25,27,35,37,(39)(40)(41) .…”

Section: Prognostic Implications Of Cytopathologic Classification Of mentioning

confidence: 99%

Prognostic implications of cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy

Augsburger¹,

Corrêa²,

Trichopoulos³

2013

Arq. Bras. Oftalmol.

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Purpose: Determine whether cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy (FNAB) is a significant prognostic factor for death from metastasis. Methods: Retrospective analysis of cases of clinically diagnosed uveal melanoma evaluated by fine-needle aspiration biopsy from 1980 to 2006. Main outcome evaluated was death from metastasis. Associations between baseline clinical variables and cytopathologic classification were evaluated using cross-tabulation. Prognostic significance of cytopathologic classification was evaluated by Ka planMeier and Cox proportional hazards analysis. Results: Of 302 studied biopsies, 260 (86.1%) yielded sufficient cells for cytopathologic classification. Eighty of the 260 patients who had a sufficient specimen have already died (P=0.021), 69 from metastatic uveal melanoma. Cell type assigned by cytopathology was strongly associated with metastasis/metastatic death in this series (P=0.0048). Multivariate analysis showed cytopathologic classification to be an independently significant prognostic factor for metastatic death (P=0.0006). None of the 42 patients whose tumor yielded insufficient aspirates (sampled in at least two sites) have developed metastasis or died of metastasis thus far. Conclusion: In this series, cytopathology of fine-needle aspiration biopsy samples obtained from uveal melanomas was strongly prognostic of death from metastasis. Insufficiently aspirates (2 or more sites sampled) proved to be prognostic of a favorable outcome (i.e., not developing metastasis). Keywords

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Clinical applications of chromosome analysis, from fine needle aspiration biopsies, of posterior uveal melanomas

Abstract: Cytogenetic analysis was performed following standard protocols.

Cited by 33 publications

References 17 publications

Chromosome 3 Analysis of Uveal Melanoma Using Fine-Needle Aspiration Biopsy at the Time of Plaque Radiotherapy in 140 Consecutive Cases

Chromosome 3 Analysis of Uveal Melanoma Using Fine-Needle Aspiration Biopsy at the Time of Plaque Radiotherapy in 140 Consecutive Cases

In vivo monosomy 3 detection of posterior uveal melanoma: 3-year follow-up

Prognostic implications of cytopathologic classification of melanocytic uveal tumors evaluated by fine-needle aspiration biopsy

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