Clinical and economic evaluation of BBL™ CHROMagar™ Salmonella (CHROMSal) versus subculture after selenite broth enrichment to CHROMSal and Hektoen enteric agars to detect enteric Salmonella in a large regional microbiology laboratory

Church, Deirdre L.; Emshey, D.; Lloyd, Tracie; Pitout, Johann

doi:10.1016/j.diagmicrobio.2010.04.003

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…3). The superior performance of chromogenic media has been demonstrated in several studies that reported better sensitivity and specificity over traditional selective/differential media used for Salmonella isolation and detection (92)(93)(94). Putative Salmonella cultures that are lactose nonfermenters and/or H 2 S producers, isolated on selective plates, are often subjected to a secondary screening test involving subculturing of the suspicious Salmonella colonies onto triple-sugar iron (TSI) agar, lysine iron agar (LIA), or Hy-Enterotest system tubes containing special culture medium that permits the identification of Enterobacteriaceae.…”

Section: Laboratory Diagnosis Of Salmonella Infections Salmonella Diamentioning

confidence: 99%

Persistent Infection and Long-Term Carriage of Typhoidal and Nontyphoidal Salmonellae

2018

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SUMMARY The ability of pathogenic bacteria to affect higher organisms and cause disease is one of the most dramatic properties of microorganisms. Some pathogens can establish transient colonization only, but others are capable of infecting their host for many years or even for a lifetime. Long-term infection is called persistence, and this phenotype is fundamental for the biology of important human pathogens, including Helicobacter pylori, Mycobacterium tuberculosis, and Salmonella enterica. Both typhoidal and nontyphoidal serovars of the species Salmonella enterica can cause persistent infection in humans; however, as these two Salmonella groups cause clinically distinct diseases, the characteristics of their persistent infections in humans differ significantly. Here, following a general summary of Salmonella pathogenicity, host specificity, epidemiology, and laboratory diagnosis, I review the current knowledge about Salmonella persistence and discuss the relevant epidemiology of persistence (including carrier rate, duration of shedding, and host and pathogen risk factors), the host response to Salmonella persistence, Salmonella genes involved in this lifestyle, as well as genetic and phenotypic changes acquired during prolonged infection within the host. Additionally, I highlight differences between the persistence of typhoidal and nontyphoidal Salmonella strains in humans and summarize the current gaps and limitations in our understanding, diagnosis, and curing of persistent Salmonella infections.

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Section: Laboratory Diagnosis Of Salmonella Infections Salmonella Diamentioning

confidence: 99%

Persistent Infection and Long-Term Carriage of Typhoidal and Nontyphoidal Salmonellae

2018

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“…The value of chromogenic media has been demonstrated in a number of studies that reported improved sensitivity and specificity over traditional selective/differential media for the recovery of Salmonella, Shigella, STEC, Vibrio, and Yersinia (267)(268)(269)(270)(271). However, these media are more expensive than traditional media and are not available for in vitro diagnostic use for Vibrio and Yersinia in the United States at this time.…”

Section: Specimen Processingmentioning

confidence: 99%

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

2015

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SUMMARY Bacterial gastroenteritis is a disease that is pervasive in both the developing and developed worlds. While for the most part bacterial gastroenteritis is self-limiting, identification of an etiological agent by bacterial stool culture is required for the management of patients with severe or prolonged diarrhea, symptoms consistent with invasive disease, or a history that may predict a complicated course of disease. Importantly, characterization of bacterial enteropathogens from stool cultures in clinical laboratories is one of the primary means by which public health officials identify and track outbreaks of bacterial gastroenteritis. This article provides guidance for clinical microbiology laboratories that perform stool cultures. The general characteristics, epidemiology, and clinical manifestations of key bacterial enteropathogens are summarized. Information regarding optimal specimen collection, transport, and processing and current diagnostic tests and testing algorithms is provided. This article is an update of Cumitech 12A (P. H. Gilligan, J. M. Janda, M. A. Karmali, and J. M. Miller, Cumitech 12A, Laboratory diagnosis of bacterial diarrhea, 1992).

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“…Biz çalışmamızda klasik besiyerleri ile daha önce karşılaş-tırma çalışmaları yapılmış, güvenilirliğini kanıtlamış bir kromojenik besiyeri olan CHROMagar Salmonella plus besiyerini kullanmayı tercih ettik [23][24][25][26] . Salmonella türü bakterilerin bu besiyerinde yaptıkları tipik koloni görünümü ve rengi (S. Typhi, S. Paratyphi A ve laktoz pozitif Salmonella bu besiyerinde pembe/leylak rengi olarak görülür) nedeni ile ayrıca zaman kaybettirici saflaştırma işlemlerine gerek kalmamaktadır 6 .…”

Section: Maldi-tof'un Başarısı Phoenix Otomatize Cihazı Ileunclassified

Salmonella Türlerinin Hızlı Tanısında Kromojenik Besiyeri İle Birlikte “Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry” Yönteminin Phoenix Otomatize Sistemi ile Kıyaslanması

Akyar¹,

Can²

2013

Kafkas Univ Vet Fak Derg

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Clinical and economic evaluation of BBL™ CHROMagar™ Salmonella (CHROMSal) versus subculture after selenite broth enrichment to CHROMSal and Hektoen enteric agars to detect enteric Salmonella in a large regional microbiology laboratory

Cited by 6 publications

References 18 publications

Persistent Infection and Long-Term Carriage of Typhoidal and Nontyphoidal Salmonellae

Persistent Infection and Long-Term Carriage of Typhoidal and Nontyphoidal Salmonellae

Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Bacterial Gastroenteritis

Salmonella Türlerinin Hızlı Tanısında Kromojenik Besiyeri İle Birlikte “Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry” Yönteminin Phoenix Otomatize Sistemi ile Kıyaslanması

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