Clickable Periodic Mesoporous Organosilica Monolith for Highly Efficient Capillary Chromatographic Separation

Abstract

A novel clickable periodic mesoporous organosilica monolith with the surface area up to 1707 m(2) g(-1) was in situ synthesized in the capillary by the one-step condensation of the organobridged-bonded alkoxysilane precursor bis(triethoxysilyl)ethylene. With Si-C bonds in the skeleton, the monolith possesses excellent chemical and mechanical stability. With vinyl groups highly loaded and homogeneously distributed throughout the structure, the monolith can be readily functionalized with functional groups by effective thiol-ene "click" chemistry reaction. Herein, with "click" modification of C18, the obtained monolith was successfully applied for capillary liquid chromatographic separation of small molecules and proteins. The column efficiency could reach 148,000 N/m, higher than most reported hybrid monoliths. Moreover, intact proteins could be separated well with good reproducibility, even after the monolithic column was exposed by basic mobile phase (pH 10.0) overnight, demonstrating the great promising of such monolith for capillary chromatographic separation.

Biodegradable Magnetic Silica@Iron Oxide Nanovectors with Ultra-Large Mesopores for High Protein Loading, Magnetothermal Release, and Delivery

“…[37] Silica NPs are slowly degraded in water by dissolution into biocompatible and bioadsorbed silicic acid products, [38] and silica NPs are renally excreted. [39][40][41] The unique properties of MSN and silica hybrid NPs have been implemented in catalysis, [37,42] separation, [43][44][45] bio-imaging, [33,[46][47][48] and in cargo delivery applications, [34][35][36][37][49][50][51] to name a few. Large-pore silica and silica hybrid NPs have also been designed for biomedical applications.…”

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

“…Zhang's group fabricated three kinds of organosilica monoliths via sol–gel process. First, a hybrid organosilica monolith with high surface area (1707 m 2 g −1 ) was synthesized in a capillary using bis‐(triethoxysilyl)ethylene as precursor and triethylamine as a basic catalyst in the presence of hexadecyltrimethylammonium chloride and a triblock polymer (F127).…”

Advances in the development and applications of organic–silica hybrid monoliths

“…A unique, large surface area (1707 m 2 /g) ethylene‐bridged hybrid silica monolith was prepared by base‐catalyzed condensation of bis‐(triethoxysilyl)ethylene and subsequent functionalization with octadecanethiol using thiol‐ene click reaction (Fig. ) . Through‐pore formation was tailored with a nonionic surfactant block copolymer, F127, and mesopores were controlled using hexadecyltrimethylammonium chloride.…”