Clearance of histamine from the peritoneal cavity of rats

Kownatzki, E.

doi:10.1007/bf01972357

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Such treatment failed to induce significant alterations in mortality (unpublished data). This method of repleting mice may, however, not be sufficient because of the low bioavailability and the lability of histamine (54). Nevertheless, our results with antihistamines and genetic knockouts strongly suggest that antihistamine could have a therapeutic value in the treatment of malaria infection, particularly by reducing the likelihood of adverse complications.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of histamine-mediated signaling confers significant protection against severe malaria in mouse models of disease

Beghdadi

Porcherie

Schneider

et al. 2008

The Journal of Experimental Medicine

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From the inoculation of Plasmodium sporozoites via Anopheles mosquito bites to the development of blood-stage parasites, a hallmark of the host response is an inflammatory reaction characterized by elevated histamine levels in the serum and tissues. Given the proinflammatory and immunosuppressive activities associated with histamine, we postulated that this vasoactive amine participates in malaria pathogenesis. Combined genetic and pharmacologic approaches demonstrated that histamine binding to H1R and H2R but not H3R and H4R increases the susceptibility of mice to infection with Plasmodium. To further understand the role of histamine in malaria pathogenesis, we used histidine decarboxylase–deficient (HDC−/−) mice, which are free of histamine. HDC−/− mice were highly resistant to severe malaria whether infected by mosquito bites or via injection of infected erythrocytes. HDC−/− mice displayed resistance to two lethal strains: Plasmodium berghei (Pb) ANKA, which triggers cerebral malaria (CM), and Pb NK65, which causes death without neurological symptoms. The resistance of HDC−/− mice to CM was associated with preserved blood–brain barrier integrity, the absence of infected erythrocyte aggregation in the brain vessels, and a lack of sequestration of CD4 and CD8 T cells. We demonstrate that histamine-mediated signaling contributes to malaria pathogenesis. Understanding the basis for these biological effects of histamine during infection may lead to novel therapeutic strategies to alleviate the severity of malaria.

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Section: Discussionmentioning

confidence: 99%

Inhibition of histamine-mediated signaling confers significant protection against severe malaria in mouse models of disease

Beghdadi

Porcherie

Schneider

et al. 2008

The Journal of Experimental Medicine

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“…Thus, the mechanisms of how systemic injection of these vasoactive peptides can modulate BLB permeability to GTTR 3 h later remain uncertain. Vasoactive peptides are also dispersed into the extravascular space, and this may mediate a prolonged effect [Kownatzki, 1984]. Vasoactive peptides may also trigger an intracellular endothelial mechanism that has a prolonged influence on the permeability of the BLB.…”

Section: Effect Of Histamine or Serotonin On The Blbmentioning

confidence: 99%

Infection-Mediated Vasoactive Peptides Modulate Cochlear Uptake of Fluorescent Gentamicin

2010

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Inflammatory mediators released during bacterial infection include vasoactive peptides such as histamine and serotonin, and their serum levels are frequently elevated. These peptides also modulate the vascular permeability of endothelial cells lining the blood-brain and blood-labyrinth barriers (BLB). These peptides may also modulate the permeability of the BLB to ototoxic aminoglycoside antibiotics prescribed to resolve bacterial sepsis. To test this hypothesis, we compared the effect of histamine and serotonin on the cochlear distribution of fluorescently conjugated gentamicin (GTTR) in control animals at 0.5, 1 and 3 h after injection of GTTR. The intensity of GTTR fluorescence was attenuated at 1 h in the histamine group compared to control mice, and more intense 3 h after injection (p < 0.05). In the serotonin group, the intensity of GTTR fluorescence was attenuated at 0.5 and 1 h (p < 0.05) and was increased at 3 h compared to control animals, where GTTR intensities peaked at 1 h and then plateaued or was slightly decreased at 3 h. This biphasic pattern of modulation was statistically significant in the apical turn of the cochlea. No difference in the intensity of GTTR fluorescence was observed in kidney proximal tubules. Systemic increases in serum levels of vasoactive peptides can modulate cochlear uptake of gentamicin, likely via permeability changes in the BLB. Conditions that influence serum levels of vasoactive peptides may potentiate aminoglycoside ototoxicity.

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“…Following removal of intraperitoneal cells as de scribed by Kownatzki [1984], Sprague-Dawley rats received an intraperitoneal injection of 10 ml HBSS which was removed after 1 h. freed of residual cells by centrifugation and either processed immediately or stored at -2 0°C .…”

Section: Rat Peritonea/ Lavage Fluidmentioning

confidence: 99%

Interference with the Fluorometric Histamine Assay by Biogenic Amines and Amino Acids

Kownatzki¹,

Grüninger²,

Fuhr³

1987

Pharmacology

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Naturally occurring low molecular weight biogenic amines and amino acids were analyzed for interference in the fluorometric histamine assay. Three mechanisms of interference were detected, mimicking of histamine, suppression of histamine fluorescence and generation of increasing histamine-like fluorescence during excitation or preirradiation of the o-phthaldialdehyde condensation product with daylight or UVA light. The latter effect was seen only with amino acids. Rat peritoneal lavage fluid contained no fluorescence-suppressing substances, but there were considerable amounts of histamine-mimicking compounds which could not be digested with a diamine oxidase, and of substances generating increasing histamine-like fluorescence when exposed to UV light after condensation with phthaldialdehyde. Although butanol extraction was highly effective in selecting histamine over interfering compounds, it was not sufficient to avoid interference. In contrast, Dowex 50 W-X8 ion exchange chromatography added little to separating histamine and interfering substances. Precautions are recommended to avoid the different types of interference when biological samples are analyzed for histamine content with the fluorometric assay.

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Clearance of histamine from the peritoneal cavity of rats

Cited by 6 publications

References 23 publications

Inhibition of histamine-mediated signaling confers significant protection against severe malaria in mouse models of disease

Inhibition of histamine-mediated signaling confers significant protection against severe malaria in mouse models of disease

Infection-Mediated Vasoactive Peptides Modulate Cochlear Uptake of Fluorescent Gentamicin

Interference with the Fluorometric Histamine Assay by Biogenic Amines and Amino Acids

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