Cleaner degreasing of sheepskins by the Yarrowia lipolytica LIP2 lipase as a chemical-free alternative in the leather industry

Moujehed, Emna; Zarai, Zied; Khemir, Haifa; Miled, Neila; Bchir, Mohamed Salah; Gablin, Corinne; Bessueille, François; Bonhommé, Anne; Léonard, Didier; Carrière, Frédéric; Aloulou, Ahmed

doi:10.1016/j.colsurfb.2021.112292

Cited by 13 publications

(6 citation statements)

References 29 publications

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“…The use of lipases in the leather industry is gaining acceptance as a safer, faster, and more economical technique. Additionally, lipases improve filling and dye penetration to give the leather a uniform appearance [ 115 ].…”

Section: Uses Of Microbial Lipases In Various Industrial Processesmentioning

confidence: 99%

“…Aloulou et al [ 116 ] reported that LIP2 lipases (YLLIP2) are produced by the yeast Yarrowia lipolytica at an industrial scale of up to 3 g/L of the culture medium. Moujehed et al [ 115 ] used YLLIP2 in the degreasing process for sheep skin instead of the harmful chemicals previously used in this process. They also reported that 6 mg of lipase/kg of raw skin resulted in successful degreasing within 15 min at a pH of 8 at 30 °C [ 115 , 117 ].…”

Section: Uses Of Microbial Lipases In Various Industrial Processesmentioning

confidence: 99%

“…Moujehed et al [ 115 ] used YLLIP2 in the degreasing process for sheep skin instead of the harmful chemicals previously used in this process. They also reported that 6 mg of lipase/kg of raw skin resulted in successful degreasing within 15 min at a pH of 8 at 30 °C [ 115 , 117 ]. Similarly, a study by Ben Rejeb et al [ 118 ] reported the optimization of enzymatic degreasing of sheep leather by using lipases from Aspergillus niger , Rhizopus oryzae , Penicillium roquefortii , and Candida rugosa in a fractional experimental design with four different parameters, viz., the source of enzyme, processing stage, amount of lipase, and duration of degreasing.…”

Section: Uses Of Microbial Lipases In Various Industrial Processesmentioning

confidence: 99%

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The Recent Advances in the Utility of Microbial Lipases: A Review

et al. 2023

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Lipases are versatile biocatalysts and are used in different bioconversion reactions. Microbial lipases are currently attracting a great amount of attention due to the rapid advancement of enzyme technology and its practical application in a variety of industrial processes. The current review provides updated information on the different sources of microbial lipases, such as fungi, bacteria, and yeast, their classical and modern purification techniques, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, aqueous two-phase system (ATPS), aqueous two-phase flotation (ATPF), and the use of microbial lipases in different industries, e.g., the food, textile, leather, cosmetics, paper, and detergent industries. Furthermore, the article provides a critical analysis of lipase-producing microbes, distinguished from the previously published reviews, and illustrates the use of lipases in biosensors, biodiesel production, and tea processing, and their role in bioremediation and racemization.

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Section: Uses Of Microbial Lipases In Various Industrial Processesmentioning

confidence: 99%

Section: Uses Of Microbial Lipases In Various Industrial Processesmentioning

confidence: 99%

Section: Uses Of Microbial Lipases In Various Industrial Processesmentioning

confidence: 99%

See 1 more Smart Citation

The Recent Advances in the Utility of Microbial Lipases: A Review

et al. 2023

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“…Lipase produced from Aspergillus oryzae is used to modify the polyethylene terephthalate (PET) fabric by increasing its polarity and anti-static ability (Mehta et al, 2017;. In another study, El Menoufy et al (2022) reported that the fungus Aspergillus niger NRRL-599 produced a lipase through solid-state fermentation and produced an immobilized lipase by using gelatin-coated titanium nanoparticles. The immobilized lipase is used in the treatment of wool after dyeing to improve color and strength.…”

Section: In the Textile Industrymentioning

confidence: 99%

Industrial applications of fungal lipases: a review

Kumar¹,

Verma²,

Dubey³

et al. 2023

Front. Microbiol.

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Fungal lipases (triacylglycerol acyl hydrolases EC 3.1.1.3) are significant industrial enzymes and have several applications in a number of industries and fields. Fungal lipases are found in several species of fungi and yeast. These enzymes are carboxylic acid esterases, categorized under the serine hydrolase family, and do not require any cofactor during the catalyzing of the reactions. It was also noticed that processes including the extraction and purification of lipases from fungi are comparatively easier and cheaper than other sources of lipases. In addition, fungal lipases have been classified into three chief classes, namely, GX, GGGX, and Y. Fungal lipases have applications not only in the hydrolysis of fats and oils (triglycerides) but are also involved in synthetic reactions such as esterification, acidolysis, alcoholysis, interesterification, and aminolysis. The production and activity of fungal lipases are highly affected by the carbon source, nitrogen source, temperature, pH, metal ions, surfactants, and moisture content. Therefore, fungal lipases have several industrial and biotechnological applications in many fields such as biodiesel production, ester synthesis, production of biodegradable biopolymers, formulations of cosmetics and personal care products, detergent manufacturing, degreasing of leather, pulp and paper production, textile industry, biosensor development, and drug formulations and as a diagnostic tool in the medical sector, biodegradation of esters, and bioremediation of wastewater. The immobilization of fungal lipases onto different carriers also helps in improving the catalytic activities and efficiencies of lipases by increasing thermal and ionic stability (in organic solvents, high pH, and temperature), being easy to recycle, and inducing the volume-specific loading of the enzyme onto the support, and thus, these features have proved to be appropriate for use as biocatalysts in different sectors.

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“…Proteases E.C.3.4 are considered one of the most important groups of enzymes produced on a commercial and industrial scale. They have a wide range of applications: in biotechnology as food additives [2], in bioremediation or biosurfactant production [3] for the recovery of biopolymers from waste, keratin, and collagen [4,5], in pharmaceuticals and cosmetics as detergents [6], and in textile technology. The rapidly developing biotechnology industry is looking for new technologies to solve existing problems, and the global production of these enzymes was worth $5 billion in 2021, with a clear upward trend, making them an important subject for research [7].…”

Section: Introductionmentioning

confidence: 99%

Extracellular activity of proteases from Yarrowia lipolytica IPS21 as a function of the carbon and nitrogen source

Wieczorek,

Miśkiewicz,

Gendaszewska

et al. 2023

Fibres &Amp; Textiles in Eastern Europe

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The yeast strain Yarrowia lipolytica IPS 21 was tested for its ability to produce the protease enzyme on analytically pure carbon sources as well as on waste carbon sources. It was confirmed that the yeast Y. lipolytica IPS21 can have a higher proteolytic activity in the presence of waste carbon sources in chrome-tanned leather shavings (CTLS) than on yeast extract alone. This is confirmed by the high concentration of amino acids in samples with CTLS, suggesting increased degradation of CTLS by Y. lipolytica or secretion of proteases into the medium. It was also confirmed that metals accumulate mainly in the biomass and not in the supernatant. The biomass was also found to contain high levels of Ca, K and P, which are essential for plant growth. These results show that Y. lipolytica strain IPS21 can be used for the production of extracellular alkaline proteases and for the degradation of protein waste.

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Cleaner degreasing of sheepskins by the Yarrowia lipolytica LIP2 lipase as a chemical-free alternative in the leather industry

Cited by 13 publications

References 29 publications

The Recent Advances in the Utility of Microbial Lipases: A Review

The Recent Advances in the Utility of Microbial Lipases: A Review

Industrial applications of fungal lipases: a review

Extracellular activity of proteases from Yarrowia lipolytica IPS21 as a function of the carbon and nitrogen source

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