Clean-up Method of Forchlorfenuron in Agricultural Products for HPLC Analysis

Abstract

A simplified method for the determination of forchlorfenuron in agricultural products by HPLC with UV detection was investigated. A chopped sample homogenate from agricultural products was extracted with acetone. The extract was filtrated and concentrated. The residues was loaded onto a Chem Elut column and extracted with ethyl acetate. The crude extract was purified on Oasis HLB and Bond Elut PSA mini-columns using a mixture of methanol and ethyl acetate. Forchlorfenuron was analyzed by HPLC with UV detection (263 nm). HPLC separation was performed on an ODS column with methanolῌwater as the mobile phase. Recoveries of forchlorfenuron from several agricultural products fortified at the level of 0.1 mg/g were in the range of 87.6ῌ99.5῍. The limit of detection (S/Nῌ3) was 0.005 mg/g in the sample.

CPPU treatment and pollination: Their combined effect on kiwifruit growth and quality

“…Recently, Negre et al (2014) reported similar dissipation behavior of CPPU in kiwifruits and a half life time of 10 days. Consequently negligible CPPU residues in kiwifruits were expected at harvest (5 months after CPPU application), as was also reported by Patterson Kobayashi et al (2007) and Negre et al (2014). All these reports support the late EU decision for amending the existing MRLs for CPPU.…”

“…In parallel, since the consumers awareness for residues of synthetic growth regulators in crops is increasing, the dissipation rate of the CPPU in the kiwifruits was determined using the QuEChERS extraction and HPLC analysis. Analytical methods including high-performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC/MS-MS) and liquid chromatography time-of-flight mass spectrometry (LC/TOF-MS) were used to determine CPPU residues in different matrices (Kobayashi et al, 2007;Valverde et al, 2007). Previous studies have also investigated its dissipation in various fruits and vegetables (Chen et al, 2013;Negre et al, 2014).…”

Application of Macroporous Diatomaceous Earth Column for Residue Analysis of Insecticide Endosulfan in Herbal Medicines

“…(Iijima et al, 1997(Iijima et al, ,2006Tseng et al, 2002;Klein and Alder, 2003;Kobayashi et al, 2007 …”

Βιοχημική Και Φυσιολογική Διερεύνηση Της Επίδρασης Εξωγενών Κυτοκινινών Στις Διεργασίες Ανάπτυξης Και Ωρίμανσης Καρπών Ακτινιδιάς

“…Σμ πενζεπυιεκμ ηςκ θαζκμθζηχκ μοζζχκ ζηα εηποθίζιαηα πμζμηζημπμζήεδηε ιε ηδ πνήζδ ηδξ ιεευδμο Folin-Ciocalteau (Singleton et al, 1999), ιεηά απυ ηαηάθδθδ ανάζςζδ (1:2.5) ιε ημ εηποθζζηζηυ. βημξ 0.5 ml ημο αναζςιέκμο εηποθίζιαημξ ακαιίπεδηακ ιε 2.5 ml ημο ακηζδναζηδνίμο Folin-Ciocalteau (αναζςιέκμ 1:10) ηαζ αημθμφεδζε πνμζεήηδ 2 ml οδαηζημφ δζαθφιαημξ Na 2 CO 3 (75 (Patterson et al, 1993;Kobayashi et al, 2007;Negre et al, 2014 (Patterson et al, 1993;Antognozzi et al, 1996), ήηακ υιςξ ιζηνυηενδ ζοβηνζηζηά ιε ηα απμηεθέζιαηα ενεοκδηχκ πμο πνδζζιμπμίδζακ ιεβαθφηενεξ ζοβηεκηνχζεζξ CPPU ζηδ δυζδ επέιααζδξ (Lawes et al, 1992;Antognozzi et al, 1997). Γεκζηά, δ επίδναζδ ημο CPPU ζημοξ ηανπμφξ αηηζκζδζάξ ελανηάηαζ ηυζμ απυ ηδ ζοβηέκηνςζδ ηδξ δναζηζηήξ μοζίαξ ζημ δζάθοια υζμ ηαζ απυ ημ πνυκμ ρεηαζιμφ (Biasi et al, 1992;Kim et al, 2006 (Iawhori et al, 1988, Patterson et al, 1993, Antognozzi et al, 1997, Kim et al, 2006 (Biedinger et al, 1990), απμηεθχκηαξ ημ ενέεζζια βζα ηδκ επαβςβή ηδξ ζφκεεζδξ θαζκμθζηχκ μοζζχκ (Dixon' and Paiva, 1995).…”