Application of Macroporous Diatomaceous Earth Column for Residue Analysis of Insecticide Endosulfan in Herbal Medicines

Abstract

BACKGROUND:Because dried herbal medicines have many active ingredients, it is not easy to determine the residue amount after extraction, partition and clean up of pesticides from them. Especially, liquid-liquid partition method is consuming many times and solvents. Macroporous diatomaceous earth(MDE) column was used to replace the separatory funnel for liquid-liquid extraction with dichloromethane to analyze the endosulfan and its metabolite. METHODS AND RESULTS:The residue analysis method using MDE column instead of liquid-liquid partition for determining insecticide endosulfan and its metabolite in 4 dried herbal medicines was developed by GC/MS. As a result, the recovery rates of the pesticides in 4 herbal medicines were ranged from 80.3 to 93.5% for α-endosulfan, from 81.0 to 100.3% for β-endosulfan and from 80.6 to 95.6% for endosulfan sulfate, respectively. The coefficients of variation for triplicate were ranged from 1.1 to 3.4%. CONCLUSION: The improved methods are more ecofriendly, safer, faster and less laborious than conventional method by KFDA.

Analysis of Fungicide Prochloraz in Platycodi Radix by GC-ECD

“…농림축산식품부에 따 르면 2020년 기준 약용작물의 재배면적은 9,792 ha이고 생 산량은 55,183 톤이며 그 중 길경의 재배면적은 597 ha으로 연간 4,391 톤의 생산량을 보였다(MAFRA, 2021). 생약 재 배 시 효과적인 병해충 방제를 위해서 대다수의 농가에서 농 약을 사용하기 때문에 생약 중 잔류농약의 분석은 필수적이 다 [6]. Prochloraz는 Imidazole계 살균제로서, 환경이나 식 물체내에서 구조가 변환되어 일반적으로 3개의 독성 대사물 을 생산하는데, N-formyl-N-propyl-N-[2-(2,4,6-trichlorophenoxy)ethyl]urea와 N-propyl-N-[2-(2,4,6-trichlorophenoxy)ethyl]urea로 전환되고 이것은 다시 2,4,6-trichlorophenol (2,4,6-T)로 분해된다 [7].…”