Clean meat: techniques for meat production and its upcoming challenges

Srutee, Rout; Sowmya, R; S, Annapure Uday

doi:10.1080/10495398.2021.1911810

Cited by 19 publications

(7 citation statements)

References 41 publications

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“…Once isolated and cultured in controlled growth conditions, MuSCs can either replicate to increase the number of cells or differentiate into multinucleated myotubes to produce cell-based meat [6]. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs [10]. The existence of contact inhibition under high-density culture condition restricts clinical or industrial-scale amplification of stem cells [11,12].…”

Section: Introductionmentioning

confidence: 99%

YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Liu

Zhu

et al. 2021

Cells

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Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show that at higher cell densities, proliferation and differentiation capacities of porcine MuSCs are impaired. We investigate the roles of Hippo-YAP signaling, which is important regulators in response to cell contact inhibition. Interestingly, abundant but not functional YAP proteins are accumulated in MuSCs seeded at high density. When treated with lysophosphatidic acid (LPA), the activator of YAP, porcine MuSCs exhibit increased proliferation and elevated differentiation potential compared with control cells. Moreover, constitutively active YAP with deactivated phosphorylation sites, but not intact YAP, promotes cell proliferation and stemness maintenance of MuSCs. Together, we reveal a potential molecular target that enables massive MuSCs expansion for large-scale cultured meat production under high-density condition.

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Section: Introductionmentioning

confidence: 99%

YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Liu

Zhu

et al. 2021

Cells

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“…Food researchers are currently developing meat analogues from different methods, such as culture‐based, plant‐based, insect‐based, fungi‐based, and algae‐based (Weinrich & Elshiewy, 2019; Srutee et al ., 2022). Plant‐based meat will be worth over $30 billion by 2026 (Zwanka, 2022).…”

Section: Introductionmentioning

confidence: 99%

A review on development of plant‐based meat analogues as future sustainable food

Rout

Sowmya

Srivastav

2023

Int J of Food Sci Tech

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SummaryConsumers worldwide want to improve their diets by consuming nutritional and eco‐friendly products in their food. The demand for plant‐based meat analogues (PBMA) has increased due to the adverse effects on animals, people, and the environment. "Meat analogue" means to use a substitute for meat as the primary element. The rising desire for "good for you" and "good for the planet" is driving the demand for plant‐based meat alternatives. This manuscript describes the methods and orientation used to create meat analogues that mimic meat. The term "bottom‐up approach" describes putting together a structure from the ground up. By systematically structuring proteins through the assembly of individual structural pieces, bottom‐up processes can approach the design of the meat. The top‐down method is more efficient and scalable, but it can only generate the required shape at massive scales. Therefore, this review discussed various production methods and their processing consequences on PBMAs proposed for creating sustainable foods to enhance their structural and techno‐functionality.

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“…According to researchers by 2050, the meat sector would need to increase its production by 50%–100% to meet the increasing population demand. The global meat output reached 263 million tons in 2018 and is projected to increase to 445 million tons by 2050 ( Srutee et al, 2021 ). The serious issue to achieving these targets are bacterial diseases and emerging resistance in bacteria which directly affect production.…”

Section: Introductionmentioning

confidence: 99%

A pharmacodynamic investigation to assess the synergism of orbifloxacin and propyl gallate against Escherichia coli

Abbas

Lee²,

Boby³

et al. 2022

Front. Pharmacol.

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Escherichia coli (E. coli) infections are becoming increasingly difficult to treat, as antibiotic-resistant variants proliferate. Studies on novel methods to combat the spread of resistance and improve the performance of current antibiotics are vital. We aimed to boost the efficacy of the antibiotic orbifloxacin (ORB) against E. coli by combining it with a phenolic component, propyl gallate (PG). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ORB against the E. coli KVCC 1423 resistant strain were 128 μg/ml and 256 μg/ml, respectively. However, the MIC of ORB for the remaining E. coli strains was 0.5 μg/ml–2 μg/ml. For the combination of PG and ORB, the lowest fractional inhibitory concentration (FIC) index was less than 0.5, and the combination decreased the MIC of both drugs by 74%. The time-kill assay revealed the killing properties of both the drugs and the pharmacodynamic model (PD model) confirmed the strong killing properties of the combination as compared to the individual activities of the drugs. The ratio between MIC and mutant prevention concentration of ORB against E. coli 1400306 and 1,423 were 1:32 and 1:8, respectively. The combination of ORB and PG showed strong biofilm eradication and inhibited the motility of bacteria. The cell viability of the combination was > 80%. Therefore, we believe that ORB and PG in combination could be a possible antibacterial candidate that could minimize resistance and improve antibiotic potential.

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Clean meat: techniques for meat production and its upcoming challenges

Cited by 19 publications

References 41 publications

YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

A review on development of plant‐based meat analogues as future sustainable food

A pharmacodynamic investigation to assess the synergism of orbifloxacin and propyl gallate against Escherichia coli

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