YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Liu, Zheng; Li, Gang; Zhu, Huixin; Wu, Zhongyuan; Ding, Xi; Hu, Rongrong; Jiang, Yichen; Tang, Changbo; Ding, Shijie; Guo, Renpeng

doi:10.3390/cells10113069

Cited by 17 publications

(14 citation statements)

References 40 publications

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“…It is consistent with the finding that inhibiting Yap1/Tead target genes in mouse embryonic neural stem cells contribute to the differentiation of premature neurons (Han et al, 2015). In pluripotent star stem cells, YAP1/TAZ is involved in maintaining cell differentiation and proliferation (Lorthongpanich et al, 2020). It is worth noting that YAP1, activated by tropomyosin-1 overexpression, maintains its nuclear localization and coaxes mesenchymal stem cell differentiation into osteogenic cells (Brielle et al, 2021).…”

Section: Yes-associated Protein 1 Mediates the Differentiation Of Ste...supporting

confidence: 89%

“…In addition, Yap1-deficient retinal progenitors displayed the decrease in S-phase cells and altered cell cycle progression, as evidenced by YAP1-mediated enhancement of the proliferation of retinal progenitors (Kim et al, 2016). In porcine muscle stem cells, YAP1 promotes their proliferation at a high cell density (Liu et al, 2021). Yap1/Taz signaling in mesenchymal progenitor indirectly regulates the proliferation of muscle stem cells (Kaneshige et al, 2022).…”

Section: Main Textmentioning

confidence: 99%

“…When MST1/2, the upstream of YAP1, is inhibited, YAP1 is phosphorylated to inhibit the differentiation of muscle satellite cells (Yang et al, 2022). Meanwhile, YAP1 activation by doxycycline can promote the differentiation of pig muscle stem cells at a high cell density (Liu et al, 2021). During osteogenesis, YAP1 is activated by tropomyosin-1 to maintain its nuclear localization and coaxes bone marrow mesenchymal stem cells (BMSCs) to differentiate into osteogenic cells (Brielle et al, 2021).…”

Section: Yes-associated Protein 1 Mediates the Differentiation Of Ste...mentioning

confidence: 99%

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The roles and regulation of Yes-associated protein 1 in stem cells

YIN¹,

LIU²,

JIANG³

et al. 2023

Biocell

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Yes-associated protein 1 (YAP1) is a downstream effector of the Hippo signaling pathway, and it is involved in tumorigenesis, tissue repair, growth, and development. In this review, the biological roles and the mechanisms of YAP1 in mediating stem cell fate decisions are discussed, including cell proliferation, differentiation, and apoptosis. In general, YAP1 promotes the proliferation and differentiation of stem cells, including embryonic stem cells and adult stem cells. It inhibits apoptosis by binding to the transcription factors, e.g., transcriptional enhanced associate domain (TEAD), Smad, runt-related transcription factor 1/2, p73, p63, and Erb84, to maintain tissue homeostasis. The translocalization of YAP1 in cellular nuclei and the phosphorylation in the cytoplasm work as important and unusual events for the activation of YAP1. Moreover, YAP1 serves as the crosstalk for the Hippo pathway and other signaling pathways, including the Wnt and Notch pathways. It is highlighted in this review that YAP1 is an essential regulator for stem cells that have significant applications in regenerative medicine and reproductive medicine.

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Section: Yes-associated Protein 1 Mediates the Differentiation Of Ste...supporting

confidence: 89%

Section: Main Textmentioning

confidence: 99%

Section: Yes-associated Protein 1 Mediates the Differentiation Of Ste...mentioning

confidence: 99%

See 1 more Smart Citation

The roles and regulation of Yes-associated protein 1 in stem cells

YIN¹,

LIU²,

JIANG³

et al. 2023

Biocell

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show abstract

“…[ 58,59 ] Its nuclear localization is associated with activation of the mechanotransduction pathway, which can lead to enhanced viability of the constructs. [ 60,61 ] We observed a higher nuclear localization of YAP in constructs incubated in CarGrow compared to those that were extracted (Figure 4H). This result indicates that the cells cultivated within CarGrow receive different mechanical cues compare to extracted scaffolds.…”

Section: Resultsmentioning

confidence: 89%

Print‐and‐Grow within a Novel Support Material for 3D Bioprinting and Post‐Printing Tissue Growth

et al. 2022

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3D bioprinting holds great promise for tissue engineering, with extrusion bioprinting in suspended hydrogels becoming the leading bioprinting technique in recent years. In this method, living cells are incorporated within bioinks, extruded layer by layer into a granular support material followed by gelation of the bioink through diverse cross‐linking mechanisms. This approach offers high fidelity and precise fabrication of complex structures mimicking living tissue properties. However, the transition of cell mass mixed with the bioink into functional native‐like tissue requires post‐printing cultivation in vitro. An often‐overlooked drawback of 3D bioprinting is the nonuniform shrinkage and deformation of printed constructs during the post‐printing tissue maturation period, leading to highly variable engineered constructs with unpredictable size and shape. This limitation poses a challenge for the technology to meet applicative requirements. A novel technology of “print‐and‐grow,” involving 3D bioprinting and subsequent cultivation in κ‐Carrageenan‐based microgels (CarGrow) for days is presented. CarGrow enhances the long‐term structural stability of the printed objects by providing mechanical support. Moreover, this technology provides a possibility for live imaging to monitor tissue maturation. The “print‐and‐grow” method demonstrates accurate bioprinting with high tissue viability and functionality while preserving the construct's shape and size.

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“…LATS1 can phosphorylate YAP, a significant Hippo pathway downstream effector, thereby inhibiting YAP nuclear translocation [ 18 , 19 ]. Together with TEAD, YAP functions in the nucleus as a transcriptional coactivator to suppress RB/p16 expression and promote the expression of cyclin, Oct-4 and vimentin, which are connected to cell proliferation, cell cycle arrest and cellular senescence [ 20 , 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer

Nan

Liu

Zeng

et al. 2022

JCM

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The purpose of this study is to study the effect of curcumin derivative WZ10 on the proliferation, invasion and apoptosis of ovarian cancer OVCAR3 cells, and to explore its mechanism. MTT assay was used to detect the effect of WZ10 on the proliferation of ovarian cancer OVCAR3 cells; Annexin V/PI double staining flow cytometry was used to detect the effect of WZ10 on cell apoptosis; Transwell method was used to detect the effect of WZ10 on cell invasiveness; Western blot was used to investigate the effect of WZ10 Mechanisms affecting OVCAR3 activity in ovarian cancer in vitro. Our results show that WZ10 treatment could significantly inhibit the proliferation and invasion of OVCAR3 cells, induce apoptosis of OVCAR3 cells in a dose-dependent manner. After knockdown of Hippo expression with sh-RNA, further combined treatment with WZ10 had no significant image on ovarian cancer OVCAR3 cells. In conclusion: WZ10 can significantly inhibit the proliferation of OVCAR3 cells, reduce cell invasion and proliferation by downregulating the activation of Hippo-YAP pathway, and induce cell apoptosis.

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YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Cited by 17 publications

References 40 publications

The roles and regulation of Yes-associated protein 1 in stem cells

The roles and regulation of Yes-associated protein 1 in stem cells

Print‐and‐Grow within a Novel Support Material for 3D Bioprinting and Post‐Printing Tissue Growth

Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer

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