Claudin-5 expression in the vasculature of the developing chick embryo

Collins, Michelle M.; Baumholtz, Amanda I.; Ryan, Aimee K.

doi:10.1016/j.gep.2012.01.005

Cited by 10 publications

(7 citation statements)

References 41 publications

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“…In both the mouse and chick, claudin-3 localizes to the nephric duct and ureteric bud, and additional experiments reveal that it plays an important role in regulating tubule formation from inner medullary collecting duct cells in vitro (Haddad et al, 2011). Chick claudin-5 expression is restricted to the extra-embryonic tissue at HH4 and 6, with embryonic expression not observed until HH8 and correlating with the onset of vascularization, a finding corroborated by documentation of later claudin-5 expression in the developing vasculature (Collins et al, 2012). Claudin-5 is also transiently expressed during the formation of the chick retinal pigment epithelium, with highest levels observed between embryonic days 10–14 (Kojima et al, 2002).…”

Section: Discussionmentioning

confidence: 67%

The tight junction protein claudin-1 influences cranial neural crest cell emigration

Fishwick

Neiderer

Jhingory

et al. 2012

Mechanisms of Development

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The neural crest is a population of migratory cells that follows specific pathways during development, eventually differentiating to form parts of the face, heart, and peripheral nervous system, the latter of which includes contributions from placodal cells derived from the ectoderm. Stationary, premigratory neural crest cells acquire the capacity to migrate by undergoing an epithelial-to-mesenchymal transition that facilitates their emigration from the dorsal neural tube. This emigration involves, in part, the dismantling of cell-cell junctions, including apically localized tight junctions in the neuroepithelium. In this study, we have characterized the role of the transmembrane tight junction protein claudin-1 during neural crest and placode ontogeny. Our data indicate that claudin-1 is highly expressed in the developing neuroepithelium but is down-regulated in migratory neural crest cells, although expression persists in the ectoderm from which the placode cells arise. Depletion or overexpression of claudin-1 augments or reduces neural crest cell emigration, respectively, but does not impact the development of several cranial placodes. Taken together, our results reveal a novel function for a tight junction protein in the formation of migratory cranial neural crest cells in the developing vertebrate embryo.

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Section: Discussionmentioning

confidence: 67%

The tight junction protein claudin-1 influences cranial neural crest cell emigration

Fishwick

Neiderer

Jhingory

et al. 2012

Mechanisms of Development

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“…Within chicks, only claudin-5 is localised to endothelial cells and is present from the onset of vasculogenesis at HH stage 4 in extraembryonic tissue. Claudin-5 expression appears in the embryo at HH stage 8 coinciding with embryonic vascularisation and by HH stage 24 is highly visible in branching blood vessels in the brain [17]. In the developing rat, claudin-5 is detected as early as E12 coinciding with angiogenesis [18].…”

Section: Expression Of Claudin-5mentioning

confidence: 99%

Claudin-5: gatekeeper of neurological function

Greene¹,

Hanley²,

Campbell³

2019

Fluids Barriers CNS

342

277

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Tight junction proteins of the blood–brain barrier are vital for maintaining integrity of endothelial cells lining brain blood vessels. The presence of these protein complexes in the space between endothelial cells creates a dynamic, highly regulated and restrictive microenvironment that is vital for neural homeostasis. By limiting paracellular diffusion of material between blood and brain, tight junction proteins provide a protective barrier preventing the passage of unwanted and potentially damaging material. Simultaneously, this protective barrier hinders the therapeutic effectiveness of central nervous system acting drugs with over 95% of small molecule therapeutics unable to bypass the blood–brain barrier. At the blood–brain barrier, claudin-5 is the most enriched tight junction protein and its dysfunction has been implicated in neurodegenerative disorders such as Alzheimer’s disease, neuroinflammatory disorders such as multiple sclerosis as well as psychiatric disorders including depression and schizophrenia. By regulating levels of claudin-5, it is possible to abrogate disease symptoms in many of these disorders. This review will give an overview of the blood–brain barrier and the role of tight junction complexes in maintaining blood–brain barrier integrity before focusing on the role of claudin-5 and its regulation in homeostatic and pathological conditions. We will also summarise therapeutic strategies to restore integrity of cerebral vessels by targeting tight junction protein complexes.

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“…Therefore, we performed a comprehensive temporal and spatial expression analysis of the claudin family in chick embryos. We have previously published detailed expression patterns of Claudin-1 and -5 24 , 25 . Here, we present the expression patterns for 14 chick claudin family members during gastrulation, neurulation and the early stages of organogenesis.…”

Section: Introductionmentioning

confidence: 95%

Claudin family members exhibit unique temporal and spatial expression boundaries in the chick embryo

2013

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The claudin family of proteins are integral components of tight junctions and are responsible for determining the ion specificity and permeability of paracellular transport within epithelial and endothelial cell layers. Several members of the claudin family have been shown to be important during embryonic development and morphogenesis. However, detailed embryonic expression patterns have been described for only a few claudins. Here, we provide a phylogenetic analysis of the chicken claudins and a comprehensive analysis of their mRNA expression profiles. We found that claudin family members exhibit both overlapping and unique expression patterns throughout development. Especially striking were the distinct expression boundaries observed between neural and non-neural ectoderm, as well as within ectodermal derivatives. Claudins were also expressed in endodermally-derived tissues, including the anterior intestinal portal, pharynx, lung and pancreas and in mesodermally derived tissues such as the kidney, gonad and heart. The overlapping zones of claudin expression observed in the chick embryo may confer distinct domains of ion permeability within the early epiblast and in epithelial, mesodermal and endothelial derivatives that may ultimately influence embryonic patterning and morphogenesis during development.

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Claudin-5 expression in the vasculature of the developing chick embryo

Cited by 10 publications

References 41 publications

The tight junction protein claudin-1 influences cranial neural crest cell emigration

The tight junction protein claudin-1 influences cranial neural crest cell emigration

Claudin-5: gatekeeper of neurological function

Claudin family members exhibit unique temporal and spatial expression boundaries in the chick embryo

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