Classification of Sequences Expressed during the Primordial and Basidiome Stages of the Cultivated Mushroom Agaricus bisporus

Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations

“…The remaining TDFs (70%) did not have significant matches within the GenBank databases and may represent undescribed fungal genes, although this could also be partly due to the fragments being derived from either the 5′ or the 3′ untranslated messenger RNA (mRNA) regions. This relatively high proportion of TDFs showing no homology to GenBank entries is compatible with the range of 50-65% reported for expressed sequence tags (ESTs) in other filamentous fungi (Lee et al 2002;Ospina-Giraldo et al 2000;Skinner et al 2001) and might reflect the low number of database entries describing sequences of fungal origin.…”

Comparative Analysis of Sequences Expressed during the Liquid-Cultured Mycelia and Fruit Body Stages of Pleurotus ostreatus

“…Therefore, redundancy analysis between mycelial ESTs and fruit body ESTs also can show developmentally specific gene expression. According to a comparison of 446 unigenes isolated from the promodia and fruiting body, 55 ESTs (12%) were common and others were specific to each stage in Agaricus bisporus (Ospina-Giraldo et al, 2000). In this study, only 66 (5.3%) among 1256 unigenes were common between mycelial and fruit body.…”

“…In highly redundant ESTs, it can be considered that the genes were preferentially expressed only in a particular tissue and EST redundancy is proportional to expressed mRNA quantity (Hwang et al, 2000;Ospina-Giraldo et al, 2000). Most stage-specific ESTs occurred one to four times but some ESTs occurred several times.…”

Differential Expression of Chitin Synthase III and IV mRNAs in Ascomata of Tuber borchii Vittad

“…However, no Chs gene has thus far been functionally linked to fruitbody development in higher fungi. To the best of our knowledge, there is only a report of an expressed sequence tag (EST) clone found in developing basidioma of Agaricus bisporus which shows homology to a class V Chs gene (Ospina-Giraldo et al, 2000).…”