Classification of pollen species using autofluorescence image analysis

Mitsumoto, Kotaro; Yabusaki, Katsumi; Aoyagi, Haruhiko

doi:10.1016/j.jbiosc.2008.10.001

Cited by 56 publications

(43 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is evident from row (A) that there is no autofl uorescence of natural spores with these settings, whereas the blue and red channels show strong autofl uorescence from the spore constituents which is even more evident from their overlay image. This autofl uorescence can be primarily attributed to the presence of sporoplasm constituents in the form of cellular organelles, [ 16,38,39 ] and suggests that a large portion of the inner cavity of the natural spores is empty.…”

Section: Macromolecular Encapsulation and Characterization Of Naturalmentioning

confidence: 99%

Lycopodium Spores: A Naturally Manufactured, Superrobust Biomaterial for Drug Delivery

Mundargi

Potroz

Park

et al. 2015

Adv Funct Materials

View full text Add to dashboard Cite

Herein, the exploration of natural plant‐based “spores” for the encapsulation of macromolecules as a drug delivery platform is reported. Benefits of encapsulation with natural “spores” include highly uniform size distribution and materials encapsulation by relatively economical and simple versatile methods. The natural spores possess unique micromeritic properties and an inner cavity for significant macromolecule loading with retention of therapeutic spore constituents. In addition, these natural spores can be used as advanced materials to encapsulate a wide variety of pharmaceutical drugs, chemicals, cosmetics, and food supplements. Here, for the first time a strategy to utilize natural spores as advanced materials is developed to encapsulate macromolecules by three different microencapsulation techniques including passive, compression, and vacuum loading. The natural spore formulations developed by these techniques are extensively characterized with respect to size uniformity, shape, encapsulation efficiency, and localization of macromolecules in the spores. In vitro release profiles of developed spore formulations in simulated gastric and intestinal fluids have also been studied, and alginate coatings to tune the release profile using vacuum‐loaded spores have been explored. These results provide the basis for further exploration into the encapsulation of a wide range of therapeutic molecules in natural plant spores.

show abstract

Section: Macromolecular Encapsulation and Characterization Of Naturalmentioning

confidence: 99%

Lycopodium Spores: A Naturally Manufactured, Superrobust Biomaterial for Drug Delivery

Mundargi

Potroz

Park

et al. 2015

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Such chemotaxonomy is possible because the tissues of plants that utilize the C 4 photosynthetic pathway, which has independently evolved at least 18 times in grasses (Edwards et al 2010), have a heavier carbon isotopic composition than the tissues of plants that use the C 3 photosynthetic pathway (Amundson et al 1997;Vogts et al 2009). Techniques based on the fluorescence properties of the exine have also been explored, and the fluorescence spectra emitted from the exine following excitation can differ between certain taxa, which has been used to classify pollen grains (Mitsumoto et al 2009;Pan et al 2011).…”

Section: The Use Of Nonmorphological Charactersmentioning

confidence: 99%

On the Taxonomic Resolution of Pollen and Spore Records of Earth’s Vegetation

Mander

Punyasena

2014

International Journal of Plant Sciences

View full text Add to dashboard Cite

Premise of research. Pollen and spores (sporomorphs) are a valuable record of plant life and have provided information on subjects ranging from the nature and timing of evolutionary events to the relationship between vegetation and climate. However, sporomorphs can be morphologically similar at the species, genus, or family level. Studies of extinct plant groups in pre-Quaternary time often include dispersed sporomorph taxa whose parent plant is known only to the class level. Consequently, sporomorph records of vegetation suffer from limited taxonomic resolution and typically record information about plant life at a taxonomic rank above species.Methodology. In this article, we review the causes of low taxonomic resolution, highlight examples where this has hampered the study of vegetation, and discuss the strategies researchers have developed to overcome the low taxonomic resolution of the sporomorph record. Based on this review, we offer our views on how greater taxonomic precision might be attained in future work.Pivotal results. Low taxonomic resolution results from a combination of several factors, including inadequate reference collections, the absence of sporomorphs in situ in fossilized reproductive structures, and damage following fossilization. A primary cause is the difficulty of accurately describing the very small morphological differences between species using descriptive terminology, which results in palynologists classifying sporomorphs conservatively at the genus or family level to ensure that classifications are reproducible between samples and between researchers.Conclusions. In our view, the most promising approach to the problem of low taxonomic resolution is a combination of high-resolution imaging and computational image analysis. In particular, we encourage palynologists to explore the utility of microscopy techniques that aim to recover morphological information from below the diffraction limit of light and to employ computational image analyses to consistently quantify small morphological differences between species.

show abstract

“…17,18 Fluorescence and bright-field micrographs of pollen grains on a glass plate were taken using a fluorescence microscope (Leica DMR; Leica Microsystems, Tokyo, Japan) equipped with both an ultraviolet illuminating system using a xenon lamp and a band-pass filter (central wavelength: 340 nm) and CCD camera (SPOT ISA-CE Color; Diagnostic Instruments, Inc, Sterling Heights, MI). Samples for scanning electron microscopy study were prepared according to the method of Vinckier and Smets.…”

Section: Microscopic Observationsmentioning

confidence: 99%

Fullerene fine particles adhere to pollen grains and affect their autofluorescence and germination

Aoyagi¹,

Ugwu²

2011

NSA

Self Cite

View full text Add to dashboard Cite

Adhesion of commercially produced fullerene fine particles to Cryptomeria japonica, Chamaecyparis obtusa and Camellia japonica pollen grains was investigated. The autofluorescence of pollen grains was affected by the adhesion of fullerene fine particles to the pollen grains. The degree of adhesion of fullerene fine particles to the pollen grains varied depending on the type of fullerene. Furthermore, germination of Camellia japonica pollen grains was inhibited by the adhesion of fullerene fine particles.

show abstract

Classification of pollen species using autofluorescence image analysis

Cited by 56 publications

References 8 publications

Lycopodium Spores: A Naturally Manufactured, Superrobust Biomaterial for Drug Delivery

Lycopodium Spores: A Naturally Manufactured, Superrobust Biomaterial for Drug Delivery

On the Taxonomic Resolution of Pollen and Spore Records of Earth’s Vegetation

Fullerene fine particles adhere to pollen grains and affect their autofluorescence and germination

Contact Info

Product

Resources

About