Classification of
            <i>Streptomyces</i>
            Spore Surfaces into Five Groups

Dietz, Alma; Mathews, John D.

doi:10.1128/am.21.3.527-533.1971

Cited by 22 publications

(2 citation statements)

References 9 publications

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“…The study of macro-morphological characteristics showed that this isolate belongs to the series of gray, this color is specific to bacteria of the genus Streptomyces (Tresner and Backus 1963). The micro-morphological characterization showed that the isolate exhibits smooth spores, and spiral type and straight to flexuous that are typical of the genus Streptomyces (Dietz and Mathews 1971). Physiologically, the isolated strain was metabolically active.…”

Section: Discussionmentioning

confidence: 87%

Molecular identification, antibacterial activity, and production of hydrolytic enzymes by halotolerant bacterium Streptomyces sp. ESM2-25 GTF strain isolated from extreme environment in north-east of Algeria

Alliouch-Kerboua

Bourzama

Gacemi-Kirane

et al. 2023

Nova Biotechnol. Chim.

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The aim of this study is to establish the taxonomic position of the halotolerant bacterium ESM2-25 GTF strain isolated from the extreme environment of El-Mellah lagoon water, which is situated in the city of El-Kala in the northeast of Algeria and to study its phenotypic characteristics, antibacterial activity against several pathogenic bacteria, and hydrolytic enzymes production. The novel bacterium ESM2-25 GTF strain was isolated from water samples by the dilution agar plating method using the starch-casein medium, screened in vitro for its hydrolytic enzymes production and antibacterial activity. The phenotypic and molecular characteristics show that the strain ESM2-25 GTF belongs to the genus Streptomyces. However, the comparison of the morphological and physiological characteristics of the strain ESM2-25 GTF with those of the nearest species showed significant differences. This strain showed an antibacterial activity against Bacillus subtilis subsp. spizizenii CIP 106094, Escherichia coli ATCC 29522, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 29213 and S. aureus MRSA ATCC 43300, while it is not active against Pseudomonas aeruginosa ATCC 27853. Furthermore, the strain ECM2-25 GTF was able to produce different hydrolytic enzymes such as lipase, protease, amylase, catalase, and gelatinase, which have applications in the field of food and industry. The interesting antibacterial activity of the strain ESM2-25 GTF against pathogenic bacteria and hydrolytic enzymes production indicate the importance of the exploitation of marine actinomycetes for biotechnological applications and the discovery of new antibacterial molecules and could encourage further research on the bioactive molecules secreted by this strain.

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Section: Discussionmentioning

confidence: 87%

Molecular identification, antibacterial activity, and production of hydrolytic enzymes by halotolerant bacterium Streptomyces sp. ESM2-25 GTF strain isolated from extreme environment in north-east of Algeria

Alliouch-Kerboua

Bourzama

Gacemi-Kirane

et al. 2023

Nova Biotechnol. Chim.

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“…Pure culture of each strain was inoculated in freshly ISP 2 broth and incubated at 28°C, 200 rpm for 2 days. The genomic DNA was extracted by physical and chemical combination methods (51). The 16S rRNA gene was amplified from the extracted genomic DNA by using the universal primers 1492R (5′ACGGCTACC TTGTTACGACTT 3′) and 27F (5′AGTTTGATCCTGGCTCAG 3′) directions.…”

Section: Genotypic Characteristics 16s Rrna Gene Sequencementioning

confidence: 99%

Antioxidant and antimicrobial activities of crude extracts of actinomycetes from soils

Wai¹

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A total of 17 actinomycetes isolated from soil collected from Nong Khai, Nakhon Ratchasima, Khon Kaen, Phichit, Chachoengsao and Udonthani Province, were evaluated on antimicrobial and antioxidant activities. They were identified as Streptomyces (16 strains) and Micromonospora (1 strain) based on their phenotypic and chemotaxonomic characteristics including 16S rRNA gene sequences analysis. Strain JA03 showed 98.95% similarity of 16S rRNA gene sequence to S. puniciscabiei DSM 41929T. Strain JA03 was found to be the novel species of genus Streptomyces based on whole genome average nucleotide identity (ANI). All these strains were evaluated on antimicrobial and antioxidant activities. Based on agar disk diffusion method, strain NE2-6 closely related to Streptomyces alboniger NRRL B 1832T, has the most potent antimicrobial activity against K.rhizophila ATCC 9341 and mild activity against S.aureus ATCC 25923. ET1-12 was effective on C. albicans ATCC 10231 and on K. rhizophila ATCC 934 and on B.subtilis ATCC 6633. ET2-2 closely related to Streptomyces diastaticus subsp. ardesiacus NRRL B 1773T exhibited moderate activity against B.subtilis ATCC 6633 and K. rhizophila ATCC 9341. CT2-4 closely related to Streptomyces capoamus JCM 4734T showed moderate effect on S.aureus ATCC 25923. Strain JA03 closely related to Streptomyces puniciscabiei DSM 41929T showed the highest phenolic content (33.4 µg GAE/ mg of extract) based on TPC assay and MKP33 closely related to Micromonospora yasonensis DS 3186T showed the highest FRAP value (135 µg AAE/ mg of extract) based on FRAP assay. Strain CT1-17 closely related to Streptomyces olivaceus NRRB L 3009T, showed potent scavenging activity (80.21±0.04%) at 1,000 µg/ml with IC50 value of 218.6±27.4 µg/ml based on DPPH assay. Strain MKP33 showed more potent in nitric oxide scavenging (49.3±1.96%) at 100 µg/ml than NE2-6 (49.6±1.34%) at 500 µg/ml. Cytotoxic effects of strains NE1-12, ET3-23, CT1-17, NE2-6, CT2-4, CT2-10, JA03 and MKP33 on RAW264.7 macrophage cells were evaluated by % cell viability. Strains NE1-12, CT1-17, JA03 and MKP33 showed no cytotoxic effect at 200µg/mL and strains CT2-4, CT2-10, ET3-23 and NE2-6 at 10 µg/ml on RAW 264.7 macrophage cells. Strain ET3-23 inhibited intracellular ROS generation (33±6.56%) in H2O2 (100µM) induced RAW 264.7 cells. CT2-10 at 10 µg/mL significantly inhibited NO production induced by LPS (100ng/ml) in RAW264.7 macrophage cells when compared with MKP33 at 200 µg/ml (13.87± 1.78µM and 2.9±1.02µM, respectively). In conclusion, these findings confirmed that Strain JA03 is the novel species of genus Streptomyces and possesses antioxidant activity. The actinomycetes isolated from soil provided the activity information in the development of good candidates for antibiotics and antioxidants.

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Diversity of Plant Associated Actinobacteria

Bouizgarne

Aouamar

2014

Sustainable Development and Biodiversity

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The phylum Actinobacteria encompasses Gram-positive bacteria with a high DNA G+C. In soils, they present multiple lifestyles: rhizosphere saprophytes, endophytes, facultative symbionts and obligate phytopathogens. They play either beneficial or adverse effects towards plants. Numerous studies focused on their taxonomy and preservation. Also, adequate strategies were developed to enable their isolation. Phenotypically, the Actinobacteria is one of the most diverse phyla within bacteria. Their presence was once monitored by classical culture dependent techniques and phenetic characterization. Development of culture independent methods including chemotaxonomic and genomic approaches such as 16S rRNA gene analysis allowed to detect the so called unculturable bacteria. Major works on their diversity combine culture dependant and independant techniques. This chapter focuses on the phenetic and genetic diversity of Actinobacteria in plant-soil systems. It begins with some knowledge of their biology and their taxonomy, followed by a brief overview of the methods that have facilitated advances in the understanding of their diversity. It also discusses diversity of ecologically important plant associated Actinobacteria (rhizosphere and phyllosphere colonizers, endophytes, symbionts and phytopathogens).

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Classification of Streptomyces Spore Surfaces into Five Groups

Abstract: Streptomyces spores surfaces have been classified into five groups, smooth, warty, spiny, hairy, and rugose, by examination of carbon replicas of spores with the transmission electron microscope and by direct examination of spores with the scanning electron microscope.

Cited by 22 publications

References 9 publications

Molecular identification, antibacterial activity, and production of hydrolytic enzymes by halotolerant bacterium Streptomyces sp. ESM2-25 GTF strain isolated from extreme environment in north-east of Algeria

Molecular identification, antibacterial activity, and production of hydrolytic enzymes by halotolerant bacterium Streptomyces sp. ESM2-25 GTF strain isolated from extreme environment in north-east of Algeria

Antioxidant and antimicrobial activities of crude extracts of actinomycetes from soils

Diversity of Plant Associated Actinobacteria

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