Classification of Human Adenoviruses by SDS-Polyacrylamide Gel Electrophoresis of Structural Polypeptides

Abstract

The virion polypeptide compositions of 15 human adenovirus types, representing all members of subgroups B and C and selected members of subgroups A and D, have been analyzed by SDS-PAGE. The capsid polypeptides II, III, Ilia and IV displayed a pattern of apparent molecular weights which was characteristic for each adenovirus serotype analyzed. Adenovirus serotypes belonging to the same subgroup were also demonstrated to share an apparent molecular weight pattern of the internal structural polypeptides in the following manner: Subgroup A: V – resolved into two bands of 46–48.5 K and 51–51.5 K; VI – 25.5 K; and VII- 18 K. Subgroup B: V- 53.5–54.5 K; VI-24 K; and VII – 18 K. Subgroup C: V – 48.5 K; VI – 24 K; and VII – 18.5 K. Subgroup D: V – 50.5 K; VI – 23.5 K; and VII – 18 K. Adenovirus type 4: V – 48 K; VI – 24.5 K; and VII – 18 K. The structural polypeptides represent a major portion of the adenovirus gene products. It is therefore proposed that the apparent molecular weight differences of viral polypeptides be used (i) to confirm the identification of an adenovirus type, and (ii) for subgroup classification of adenoviruses and, consequently, that adenovirus type 4 should be classified as a member of a separate subgroup designated E.

Monoclonal Antibodies against Adenovirus Type 5: Preparation and Preliminary Characterization

“…wt. of the hexon polypeptides of the different serotypes in these experiments are consistent with those described in previous publications (Russell & Skehel, 1973;Wadell, 1979). On the whole, the fluorescent antibody (FA) technique gave a much sharper distinction between subgroup and group specificities (as demonstrated by the ability to stain type 3-and type 8-infected cells) (Table 2) whereas the immunopre.cipitation method showed a marked gradient in the abilities to precipitate hexon polypeptides of different serotypes (Fig.…”

“…2) and immunoprecipitation techniques using cells infected with adenoviruses representative of the various antigenic subgroups. Thus, types I and 2 viruses are members of the same subgroup as type 5 (group C) whereas types 3 (group B) and 12 (group A) belong to different subgroups (Green et al, 1979;Wadell, 1979). In this way, type, subgroup and group epitopes associated with the virus polypeptides could be recognized.…”

Identification of Polypeptides of the Tree Shrew (<i>Tupaia</i>) Adenovirus

“…Gel patterns of polypeptides of human and of different animal adenoviruses have been re ported by several laboratories [8][9][10][11][12][13][14]. Inspec tion of the published polypeptide patterns in 12 or 15% gels of various adenoviruses and comparison with those of TAV revealed two major differences.…”

Characterization of candidate adenovirus 37 by SDS‐polyacrylamide gel electrophoresis of virion pplypeptides and DNA restriction site mapping

“…In addition, this new adenovirus, candidate Ad 37, carries unique antigenic determinants. This is most apparent with antisera t o Ad 37, which by neutralization show virtually no cross-reactivity to any of the 36 prototype viruses [de Jong et al, 19811 , Biochemically, adenoviruses can be identified and classified either by virion polypeptide analysis [Wadell, 1979;Wadell et al, 19801 or by DNA restriction [Wadell et al, 19801. The prototype strain and five Swedish isolates of Ad 37 were therefore analysed by these techniques.…”