Monoclonal Antibodies against Adenovirus Type 5: Preparation and Preliminary Characterization

Abstract

SUMMARYEight different hybridoma cell lines producing monoclonal antibodies against the major antigens of human adenovirus type 5 have been obtained. They were selected by screening initial hybridomas by the fluorescent antibody technique followed by radioimmune precipitation and they reacted with hexon, penton, fibre and 100K polypeptides. Five apparently different epitopes against the hexon antigen were detected showing a spectrum of activity against the hexons of other serotypes, suggesting that the hexon contained a variety of subgroup specificities as well as the previously described group and type specificities.

Evidence for the Persistence of Paramyxoviruses in Human Bone Marrows

“…The slides were again washed three times in PBS and 5-gl drops of an optimal dilution of fluorescein-conjugated anti-mouse globulin (Nordic), previously adsorbed with human bone marrow cells were added and incubated as above. The slides were washed again, mounted and analysed by fluorescence microscopy (Russell et al, 1981).…”

“…A third sample was mixed with the suspension of Vero cells but in the presence of polyethylene glycol (PEG 4000; Serva, Heidelberg, F.R.G.) (Russell et al, 1981) to promote fusion of the bone marrow and tissue culture cells. After fusion the cells were placed on the multispot slides and in the plastic tissue culture wells as described above.…”

ADP-ribosylation in in vitro Systems Synthesizing Adenovirus DNA

“…Russell et al (1981) prior to analysis by SDS-PAGE and autoradiography; lane 2, as for lane 1 but immunoprecipitated with normal hamster serum. (c) CK extracts from infected cells labelled as described for samples shown in (a).…”

Detection of diphtheria toxin in culture supernates of Corynebacterium diphtheriae and C. ulcerans by immunoassay with monoclonal antibody

“…Strains were grown in 3 ml of either proteose peptone maltose (PPM) medium' ' (Proteose Peptone No. 3 [Difco] 4% w/v, maltose 0.6% w/v), or Elek broth' (proteose peptone 2% w/v, maltose 0.3% w/v, NaClO-5% w/v, lactic acid 0.07% w/v, NaOH 0.06% w/v). After incubation for 18-24 h at 37"C, cultures were centrifuged to remove the bacteria and the supernates were filtered through a 0.2-pm Acrodisc R filter (Gelman Sciences).…”