Classification of FABP isoforms and tissues based on quantitative evaluation of transcript levels of these isoforms in various rat tissues

Yamamoto, Toshiharu; Yamamoto, Atsushi; Watanabe, Masahiro; Matsuo, Taisuke; Yamazaki, Naoshi; Kataoka, Masatoshi; Terada, Hiroshi; Shinohara, Yasuo

doi:10.1007/s10529-009-0065-7

Cited by 33 publications

(30 citation statements)

References 19 publications

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“…Among these FABPs, FABP6 have been found to be highly expressed in the ileum of rats and the distal intestine of zebrafish (Yamamoto et al 2009;Du 2011). In this study, its expression pattern in the intestine is much closer to that of zebrafish than to that of mammals (Supp .…”

Section: Discussionmentioning

confidence: 99%

Division of Chinese soft‐shelled turtle intestine with molecular markers is slightly different from the morphological and histological observation

Zhang

Song

Xiao

et al. 2018

Integrative Zoology

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The Chinese soft-shelled turtle (Pelodiscus sinensis) is a commercially important species in Asian countries. Knowledge of its nutritional requirements and physiology is essential for determining the appropriate content of the feed for this animal. However, the lack of functional characterization of the intestine of this turtle limits the understanding of its absorption and utilization of nutritional materials. To solve this problem, this work utilized anatomical and histological methods to characterize 9 segments sampled along the anterior-posterior axis of the intestine. Furthermore, 9 genes, which have been well documented in the intestine division of mammals and fish, were employed to functionally characterize the 9 sampled segments. Our results suggest that regions covering from the starting site to S3 (position at 29.9% of the total length from the starting of the intestine) are the equivalent of mammalian dedumonen, and those covering S4 (40.2%) and S5 (65.4%), posterior to S8 (92.7%), are the equivalent of the mammalian ileum and the large intestine, respectively. As to the region spaning S6 (81.3%) and S7 (87.3%), its functional equivalent (small intestine or large intestine) may be variable and depends on the functional genes. This molecular characterization in relation to the division of the intestine of Chinese soft-shelled turtle may contribute to the understanding of the nutritional physiology of the turtle, and promote Chinese soft-shelled turtle production.

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Section: Discussionmentioning

confidence: 99%

Division of Chinese soft‐shelled turtle intestine with molecular markers is slightly different from the morphological and histological observation

Zhang

Song

Xiao

et al. 2018

Integrative Zoology

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“…FABPs are ubiquitously expressed proteins with the various members of this family differing in tissue expression patterns (Haunerland & Spener 2004, Yamamoto et al 2009). The present quantitative study in the mouse testicular tissue showed, to our knowledge for the first time, cell-compartmentalized and timedependent variations in the gene expression of five simultaneously assessed FABP members, which is indicative of a differential regulation of their expression at specific stages of spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…They promote the uptake and intracellular transport of fatty acids, guide them to specific metabolic pathways, and participate in cell growth by regulating the expression of specific genes (Haunerland & Spener 2004, Furuhashi & Hotamisligil 2008. In mammals, ten FABP members with different tissue-dependent patterns of expression have been described (Yamamoto et al 2009). All FABPs have the same primary role with potential differences in ligand selectivity for long-chain fatty acids, binding affinity, and binding mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…The predominant FABP in testis is widely accepted to be FABP9 (Kido & Namiki 2000, Selvaraj et al 2010, although other members of this family have also been reported, such as FABP3 (Watanabe et al 1991), FABP5 (Kingma et al 1998), and more recently FABP12 (Liu et al 2008, Yamamoto et al 2009). However, no studies are available comparing on the same basis the expression of different FABPs at definite periods of testis development and correlating this with their relative abundances, in particular testicular cell types.…”

Section: Introductionmentioning

confidence: 99%

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Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases, and perilipin 2

Oresti¹,

García-López²,

Aveldaño³

et al. 2013

Reproduction

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Male germ cell differentiation entails the synthesis and remodeling of membrane polar lipids and the formation of triacylglycerols (TAGs). This requires fatty acid-binding proteins (FABPs) for intracellular fatty acid traffic, a diacylglycerol acyltransferase (DGAT) to catalyze the final step of TAG biosynthesis, and a TAG storage mode. We examined the expression of genes encoding five members of the FABP family and two DGAT proteins, as well as the lipid droplet protein perilipin 2 (PLIN2), during mouse testis development and in specific cells from seminiferous epithelium. Fabp5 expression was distinctive of Sertoli cells and consequently was higher in prepubertal than in adult testis. The expression of Fabp3 increased in testis during postnatal development, associated with the functional differentiation of interstitial cells, but was low in germ cells. Fabp9, together with Fabp12, was prominently expressed in the latter. Their transcripts increased from spermatocytes to spermatids and, interestingly, were highest in spermatid-derived residual bodies (RB). Both Sertoli and germ cells, which produce neutral lipids and store them in lipid droplets, expressed Plin2. Yet, while Dgat1 was detected in Sertoli cells, Dgat2 accumulated in germ cells with a similar pattern of expression as Fabp9. These results correlated with polyunsaturated fatty acid-rich TAG levels also increasing with mouse germ cell differentiation highest in RB, connecting DGAT2 with the biosynthesis of such TAGs. The age-and germ cell type-associated increases in Fabp9, Dgat2, and Plin2 levels are thus functionally related in the last stages of germ cell differentiation.

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“…Nonenzymatic cytosolic FABPs are abundantly expressed in numerous animal tissues with high rates of fatty acid metabolism. FABP has 10 isoforms that are distinctly distributed in various tissues in mammals and that have distinct mechanisms in different organs and cells (5). Among them, heart-type (H)-FABP, also referred to as FABP3, is most predominantly expressed in the heart and less in the skeletal muscle, kidney, stomach and testis (5).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of heart-type fatty acid binding protein enhances fatty acid-induced podocyteï¿½injury

et al. 2017

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Abstract. Deregulated lipid metabolism is a characteristic of metabolic diseases including type 2 diabetes and obesity, and likely contributes to podocyte injury and end-stage kidney disease. Heart-type fatty acid binding protein (H-FABP) was reported to be associated with lipid metabolism. The present study investigated whether H-FABP contributes to podocyte homeostasis. Podocytes were transfected by lentiviral vector to construct a cell line which stably overexpressed H-FABP. Small interfering RNA capable of effectively silencing H-FABP was introduced into podocytes to construct a cell line with H-FABP knockdown. Certain groups were treated with palmitic acid (PA) and the fat metabolism, as well as inflammatory and oxidative stress markers were measured. PA accelerated lipid metabolism derangement, inflammatory reaction and oxidative stress in podocytes. Overexpression of H-FABP enhanced the PA-induced disequilibrium in podocytes. The mRNA and protein expression levels of acyl-coenzyme A oxidase 3 and monocyte chemotactic protein 1, and the protein expression levels of 8-hydroxy-2'-deoxyguanosine and 4-hydroxynonenal were upregulated in the H-FABP overexpression group, while the mRNA and protein expression of peroxisome proliferator activated receptor α was downregulated. Knockdown of H-FABP inhibited the PA-induced injury and lipid metabolism derangement, as well as the inflammatory reaction and oxidative stress in podocytes. These results indicated that overexpression of H-FABP enhances fatty acid-induced podocyte injury, while H-FABP inhibition may represent a potential therapeutic strategy for the prevention of lipid metabolism-associated podocyte injury.

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Classification of FABP isoforms and tissues based on quantitative evaluation of transcript levels of these isoforms in various rat tissues

Cited by 33 publications

References 19 publications

Division of Chinese soft‐shelled turtle intestine with molecular markers is slightly different from the morphological and histological observation

Division of Chinese soft‐shelled turtle intestine with molecular markers is slightly different from the morphological and histological observation

Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases, and perilipin 2

Overexpression of heart-type fatty acid binding protein enhances fatty acid-induced podocyteï¿½injury

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