Classification of commercial Catuaba samples by NMR, HPLC and chemometrics

Abstract

For over a century, Catuaba has been used in Brazilian folk medicine as an aphrodisiac even though the identity of the plant material employed is often uncertain. The species recommended by the Brazilian Pharmacopeia is Anemopaegma arvense (Bignoniaceae), but many other plants, regionally known as Catuaba, are commercialised. Frequently, the quality control of such a complex system is based on chemical markers that do not supply a general idea of the system. With the advent of the metabolomics approach, a global analysis of samples becomes possible. It appears that (1)H-NMR is the most useful method for such application, since it can be used as a wide-spectrum chemical analysis technique. Unfortunately, the generated spectra is complex so a possible approach is to look at the metabolite profile as a whole using multivariate methods, for example, by application of principal component analysis (PCA). In the present paper, we describe for the first time a proton high-resolution magic angle spinning nuclear magnetic resonance ((1)H-HR-MAS NMR) method coupled with PCA for the metabolomic analysis of some commercial Catuaba samples, which provided a reduction in the time required for such analysis. A comparative study of HPLC, HR-MAS and liquid-NMR techniques is also reported.

Phenylpropanoid substituted flavan-3-ols from Trichilia catigua and their in vitro antioxidative activity

“…Marques 2 described the differences among the species known as "catuaba" and determined that T. catigua is the main species that is sold as "catuaba" in Brazil. The same conclusion was reached by Kletter et al 3 and Daolio et al 4 The bark of T. catigua has been traditionally used as a health and mental tonic, and especially as a sexual stimulant. 3,5 Some pharmacological studies of this bark have reported antimicrobial, trypanocidal, antinociceptive, vasorelaxant or dopamine-mediated antidepressantlike effects.…”

“…Furthermore, the NOESY NMR spectrum shows no effect between the aromatic ring systems, and H-2' and H-7", confirming the absolute configuration as R. Thus, it was concluded that the structure of compound 7 corresponds to apocynin E. To the best of our knowledge, 7 has been isolated for the first time from a natural source. 4 Hz) for 8a, respectively, in comparison to the equivalent protons in procyanidin B2 supported the S-configuration at C-7". In addition, the 4b → 8-linkage and thus the R-configuration at C-4(C) was confirmed by the strong positive Cotton effect observed between 220 and 230 nm, and 280 nm in the CD spectrum.…”

Análise farmacognóstica de amostras de drogas vegetais psicoativas comercializadas em Diadema

“…Coloração: azul de Astra/safranina (B, C, F, G); lugol (C). (DAOLIO et al, 2008;MOBOT, 2011;TABANCA et al, 2007).…”

“…A confusão na identificação iniciou-se no início do século 20 com uma tese de doutorado e perdura até os dias atuais (DAOLIO et al, 2008;KLETTER et al, 2004;TABANCA et al, 2007). DAOLIO et al, 2008;KLETTER et al, 2004).…”

“…As análises, em conjunto, permitiram verificar que os quatro lotes de catuaba se tratavam da espécie T. catigua, fato que também foi anteriormente observado na literatura (BELTRAME et al, 2006;DAOLIO et al, 2008), apesar da 1ª edição da Farmacopéia (1929), revogada, oficializar órgãos subterrâneos de A. mirandum. Kletter et al (2004) realizaram um projeto de avaliação da qualidade de amostras comerciais de catuaba, onde foi possível confirmar a prevalência das cascas de caule de T. catigua dentre as DVs presentes no mercado.…”