Classification of Bifidobacterium Isolates from Infant Faeces Using PCR-Based and 16S rDNA Partial Sequences Analysis Methods

Mustafa, Shuhaimi; Ali, Abdul Manaf; Saleh, Norihan Mohd; Yazid, A.M.

doi:10.12938/bifidus1996.21.155

Cited by 12 publications

(10 citation statements)

References 20 publications

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“…The weak stimulation of bifidobacteria growth observed in this study may be explained by the fact that the stimulation effect of inulin or FOS is strain-specific [ 23 , 24 ]. Bifidobacterium isolates collected from healthy infants in Serdang, Malaysia mainly belong to the species B. pseudocatenulatum [ 25 ]. Among the bifidobacteria strains investigated, B. pseudocatenulatum demonstrated only weak growth on inulin in vitro [ 24 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Changes in Infants Faecal Characteristics and Microbiota by Inulin Supplementation

Yap

Mohamed

Jamal

et al. 2008

J. Clin. Biochem. Nutr.

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Summary The effects of inulin on the microbial composition and faecal characteristics in 36 healthy, formula-fed infants (average age 7.7 months) given 3 different daily dosages of native inulin (0.75 g/day, 1.00 g/day, and 1.25 g/day) were studied. At all levels of inulin consumption, a significant (p<0.05) reduction of potential pathogenic microorganisms such as clostridia was found. An intake of 1.25 g/day of inulin caused a significant (p<0.05) increase of Bifidobacterium spp. as well as a significant (p<0.05) decline in Gram-positive cocci and coliform bacteria. Inulin consumption resulted in a significant (p<0.05) decrease in faecal pH value and changes in faecal weight, faecal texture and colour, indicating improvement in healthy bile production and bacterial fermentation. It is concluded that inulin consumption in formula-fed infants after weaning positively affected the microbial composition of faeces and faecal properties.

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Section: Discussionmentioning

confidence: 99%

Changes in Infants Faecal Characteristics and Microbiota by Inulin Supplementation

Yap

Mohamed

Jamal

et al. 2008

J. Clin. Biochem. Nutr.

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“…pseudocatenulatum G4 is a human-derived strain obtained from the stock culture collection of Biotechnology and Functional Food Laboratory (Faculty of Food Science and Technology, UPM, Malaysia). The strain was previously classified using 16S rDNA partial sequences analysis (Shuhaimi et al 2002). The strain was maintained at )20°C in 20% (v ⁄ v) glycerol and skim milk supplemented with yeast extract.…”

Section: Maintenance Of Micro-organismmentioning

confidence: 99%

“…1995). Strain G4 of this species was screened and identified by Shuhaimi et al. (2002), found to survive the harsh gut environment (Wong et al.…”

Section: Introductionmentioning

confidence: 99%

“…Bifidobacterium pseudocatenulatum is present in the gut of breast-fed infants (Kleesen et al 1995). Strain G4 of this species was screened and identified by Shuhaimi et al (2002), found to survive the harsh gut environment (Wong et al 2006) and safe in animal test (Kabeir et al (FOS), sorbitol and arabinan selectively stimulate the growth and ⁄ or activity of indigenous probiotic bifidobacteria and lactobacillus in the intestinal tract (Gibson and Roberfroid 1995;de Vaux et al 2002;Al-Tamimi et al 2006). These probiotic bacteria have the ability to produce enzymes to hydrolyse prebiotics and elevate the level of organic acid such as acetate, propionate, butyrate and lactate.…”

Section: Introductionmentioning

confidence: 99%

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Synbiotics growth optimization ofBifidobacterium pseudocatenulatumG4 with prebiotics using a statistical methodology

Mustafa

Kabeir

Yazid

et al. 2009

Journal of Applied Microbiology

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Aims: This study demonstrated the optimum growth of Bifidobacterium pseudocatenulatum G4 with prebiotics via statistical model. Methods and Results: Commercial prebiotics [inulin and fructooligosaccharide (FOS)], together with sorbitol, arabinan and inoculum rate, were tested by fractional factorial design to determine their impact on growth of Bif. pseudocatenulatum G4 in skim milk. At 48 h incubation, bacterial growth was mainly influenced by FOS and inoculum rate. Growth reduction was observed in all samples incubated for 72 h. Central composite design (CCD) was adopted using FOS and inoculum rate at 48 h incubation to develop the statistical model for optimization. The model predicted that 2·461 log CFU ml−1 produced the optimum growth increase of Bif. pseudocatenulatum G4. The combination that produced the optimum point was 2·86% FOS (g/v) and 0·67% inoculum rate (v/v). Conclusion: At optimum combination of inoculum rate and FOS, validation experiments recorded 2·40 ± 10·02 log CFU ml−1. The application in 1‐l bioreactor for 24 h showed higher growth increase of 2·95 log CFU ml−1. Significant and Impact of the Study: Response surface methodology approach is useful to develop optimum synbiotics combination for strain G4 with FOS.

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“…More recent publications have been concerned with the characterization of fructose-6-phosphate phosphoketolase enzyme from Bifidobacterium asteroides [93]; the cloning and sequencing of the bile salt hydrolase gene from Bif. longum [94]; and molecular techniques to fingerprint isolates of Bifidobacterium and other probiotic bacteria [95][96].…”

Section: Malaysiamentioning

confidence: 99%

Probiotic Research in Australia, New Zealand and the Asia-Pacific Region

Crittenden¹,

Bird²,

Gopal

et al. 2005

CPD

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Although the epicentres of probiotic research in the past decade have been Japan and Europe, researchers in the Asia-Pacific region have actively contributed to the growing understanding of the intestinal microbial ecosystem, and interactions between gut bacteria, diet and health of the human host. A number of new probiotic strains have been developed in the region that have been demonstrated to have beneficial impacts on health in animal and human trials, including improved protection against intestinal pathogens and modulation of the immune system. Probiotics targeted to animals, including aquaculture, feature heavily in many Asian countries. Developments in probiotic technologies have included microencapsulation techniques, antimicrobial production in fermented meats, and synbiotic combinations. In particular, the impact of resistant starch on the intestinal environment and fermentation by intestinal bacteria has been intensively studied and new probiotic strains selected specifically for synbiotic combinations with resistant starch. This paper provides an overview of probiotic research within Australia, New Zealand and a number of Asian countries, and lists scientists in the Asia-Pacific region involved in various aspects of probiotic research and development.

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Classification of Bifidobacterium Isolates from Infant Faeces Using PCR-Based and 16S rDNA Partial Sequences Analysis Methods

Cited by 12 publications

References 20 publications

Changes in Infants Faecal Characteristics and Microbiota by Inulin Supplementation

Changes in Infants Faecal Characteristics and Microbiota by Inulin Supplementation

Synbiotics growth optimization ofBifidobacterium pseudocatenulatumG4 with prebiotics using a statistical methodology

Probiotic Research in Australia, New Zealand and the Asia-Pacific Region

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