Classification and Regression Trees for Bacterial Vaginosis Diagnosis in Pregnant Women Based on High-Throughput Quantitative PCR

Loquet, Aurore; Guern, Rémi Le; Grandjean, Teddy; Duployez, Claire; Bauduin, Marvin; Kipnis, Éric; Brabant, G.; Subtil, D.; Dessein, Rodrigue

doi:10.1016/j.jmoldx.2020.11.004

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…BV is typified by reduced Lactobacillus abundance in the VMC, resulting in an increased pH > 4.5 and a commensurate increase in diversity 14 . BV is most often associated with an abundance of Gardnerella vaginalis in CST IV, often accompanied with an increase in the number of anaerobes, Bacteroides and Prevotella, and often a range of potential pathogens including Ureaplasma and Gram-positive Mobiluncus and Megasphaera [15][16][17] . Although early studies identified Gardnerella vaginalis as the primary cause of BV, it is now recognized that the vaginal bacterial community varies widely in different regions and across different genetic backgrounds 18 .…”

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confidence: 99%

Microbial diversity in the vaginal microbiota and its link to pregnancy outcomes

Baud,

Hillion,

Plainvert

et al. 2023

Sci Rep

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The vaginal microbiota refers to the microorganisms that reside in the vagina. These microorganisms contribute significantly to a woman’s reproductive and general health. A healthy vaginal microbiota is typically a low-diversity environment with a predominance of lactic acid-producing Lactobacillus species. Factors such as antibiotic use, sexual activity, and hormonal changes can disrupt the balance of the vaginal microbiota, leading to conditions such as bacterial vaginosis. The composition of the vaginal microbiota changes and takes on added importance during pregnancy, serving as a barrier against infection for both mother and fetus. Despite the importance of the microorganisms that colonize the vagina, details of how changes in composition and diversity can impact pregnancy outcomes is poorly understood. This is especially true for woman with a high prevalence of Gardnerella vaginalis. Here we report on a diverse cohort of 749 women, enrolled in the InSPIRe cohort, during their final trimester of pregnancy. We show that Lactobacilli, including L. crispatus are important in maintaining low diversity, and that depletion in this critical community is linked with preterm delivery. We further demonstrate that it is overall diversity of the vaginal microbiota, not specific species, which provides the best indicator of risk.

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confidence: 99%

Microbial diversity in the vaginal microbiota and its link to pregnancy outcomes

Baud,

Hillion,

Plainvert

et al. 2023

Sci Rep

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“…Urinary tract infections are mostly brought on by Escherichia coli [ 2 ]. In addition, it has been also reported that Staphylococcus aureus is another microorganism causing BV [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment

et al. 2023

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Bacterial vaginosis is an infectious disease that has significantly affected women’s health. Metronidazole has been widely used as a drug for treating bacterial vaginosis. Nevertheless, the currently available therapies have been found to be inefficient and inconvenient. Here, we developed the combination approach of gel flake and thermoresponsive hydrogel systems. The gel flakes were prepared using gellan gum and chitosan, showing that the incorporation of metronidazole was able to provide a sustained release pattern for 24 h with an entrapment efficiency of >90%. Moreover, the gel flakes were incorporated into Pluronics-based thermoresponsive hydrogel using the combination of Pluronic F127 and F68. The hydrogels were found to exhibit the desired thermoresponsive properties, showing sol-gel transition at vaginal temperature. Following the addition of sodium alginate as a mucoadhesive agent, the hydrogel was retained in the vaginal tissue for more than 8 h, with more than 5 mg of metronidazole retained in the ex vivo evaluation. Finally, using the bacterial vaginosis infection model in rats, this approach could decrease the viability of Escherichia coli and Staphylococcus aureus with reduction percentages of more than 95% after 3 days of treatment, with the healing ability similar to normal vaginal tissue. In conclusion, this study offers an effective approach for the treatment of bacterial vaginosis.

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Self-correction of cycle threshold values by a normal distribution–based process to improve accuracy of quantification in real-time digital PCR

Zang,

Xu,

et al. 2024

Anal Bioanal Chem

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Classification and Regression Trees for Bacterial Vaginosis Diagnosis in Pregnant Women Based on High-Throughput Quantitative PCR

Cited by 6 publications

References 33 publications

Microbial diversity in the vaginal microbiota and its link to pregnancy outcomes

Microbial diversity in the vaginal microbiota and its link to pregnancy outcomes

Development of Thermoresponsive Hydrogels with Mucoadhesion Properties Loaded with Metronidazole Gel-Flakes for Improved Bacterial Vaginosis Treatment

Self-correction of cycle threshold values by a normal distribution–based process to improve accuracy of quantification in real-time digital PCR

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