Class III Peroxidases in Response to Multiple Abiotic Stresses in Arabidopsis thaliana Pyrenean Populations

Abstract

Class III peroxidases constitute a plant-specific multigene family, where 73 genes have been identified in Arabidopsis thaliana. These genes are members of the reactive oxygen species (ROS) regulatory network in the whole plant, but more importantly, at the root level. In response to abiotic stresses such as cold, heat, and salinity, their expression is significantly modified. To learn more about their transcriptional regulation, an integrative phenotypic, genomic, and transcriptomic study was executed on the roots of A. thaliana Pyrenean populations. Initially, the root phenotyping highlighted 3 Pyrenean populations to be tolerant to cold (Eaux), heat (Herr), and salt (Grip) stresses. Then, the RNA-seq analyses on these three populations, in addition to Col-0, displayed variations in CIII Prxs expression under stressful treatments and between different genotypes. Consequently, several CIII Prxs were particularly upregulated in the tolerant populations, suggesting novel and specific roles of these genes in plant tolerance against abiotic stresses.

MicroRNA397 regulates tolerance to drought and fungal infection by regulating lignin deposition in chickpea root

“…The primary root tips of the drought-treated samples were very thin and contained smaller cells than the control root tips (Supporting Information: Figure S2c,d). Drought or salinity-induced lignin deposition in the root vascular tissues of crop plants has been reported before (Fan et al, 2006;Jbir et al, 2001). Phloroglucinol staining of the longitudinal sections of the root zone 5-6 cm below the root-shoot junction displayed increased lignin deposition in drought-treated samples.…”

“…Monolignol polymerisation in the cell wall is catalysed by two groups of enzymes, LACCASES (LACs) and PEROXIDASES (PERs). PERs are a multigene family with 73 homologous genes in Arabidopsis (Eljebbawi et al, 2022; Kim et al, 2012; Valério et al, 2004). PERs use hydrogen peroxide for oxidation of their substrates (Marjamaa et al, 2009; Takahama, 1995).…”

“…PERs are a multigene family with 73 homologous genes in Arabidopsis (Eljebbawi et al, 2022;Kim et al, 2012;Valério et al, 2004). PERs use hydrogen peroxide for oxidation of their substrates (Marjamaa et al, 2009;Takahama, 1995).…”

ROS Consumers or Producers? Interpreting Transcriptomic Data by AlphaFold Modeling Provides Insights into Class III Peroxidase Functions in Response to Biotic and Abiotic Stresses

“…Interestingly, PERs function in both stress reception and responses, and specific PER expressions are induced by various biotic and abiotic stresses, such as pathogens, metals, ozone, temperature, anoxia, and nutrient deficiencies [5,10]. PERs are also known to play a central role in plant defense both by cell wall cross-linking and by producing large amounts of toxic ROS in an oxidative burst-part of the hypersensitive response in plants [5,11,12].…”

ROS and Abiotic Stress in Plants 2.0

“…The research papers included in this Special Issue add relevant pieces of the puzzle. They expand our knowledge on components of the antioxidant defenses like peroxidases and ascorbate peroxidase [2,3], transcriptional regulators from different plants that exert roles in the responses to various stresses [4][5][6], common and specific responses to various abiotic stress stimuli in a susceptible and tolerant plant [7], and even the transgenerational pervasiveness of stress memory induced by spaceflight [8]. Additionally, the three published review papers provide comprehensive summaries on the current state-of-the art of our understanding of the interference of ROS homeostasis and other signals [9][10][11].…”