The eukaryotic initiation factor 3 (eIF3) plays an important role in translation initiation, acting as a docking site for several eIFs that assemble on the 40S ribosomal subunit. Here, we use mass spectrometry to probe the subunit interactions within the human eIF3 complex. Our results show that the 13-subunit complex can be maintained intact in the gas phase, enabling us to establish unambiguously its stoichiometry and its overall subunit architecture via tandem mass spectrometry and solution disruption experiments. Dissociation takes place as a function of ionic strength to form three stable modules eIF3(c:d:e:l:k), eIF3(f:h:m), and eIF3(a:b:i:g). These modules are linked by interactions between subunits eIF3b:c and eIF3c:h. We confirmed our interaction map with the homologous yeast eIF3 complex that contains the five core subunits found in the human eIF3 and supplemented our data with results from immunoprecipitation. These results, together with the 27 subcomplexes identified with increasing ionic strength, enable us to define a comprehensive interaction map for this 800-kDa species. Our interaction map allows comparison of free eIF3 with that bound to the hepatitis C virus internal ribosome entry site (HCV-IRES) RNA. We also compare our eIF3 interaction map with related complexes, containing evolutionarily conserved protein domains, and reveal the location of subunits containing RNA recognition motifs proximal to the decoding center of the 40S subunit of the ribosome.hepatitis C virus internal ribosome entry site ͉ subunit organization model3 ͉ top-down analysis of macromolecular complexes ͉ translation regulation ͉ in-solution disruption S ince its identification in the 1970s (1-3), the translation initiation factor eIF3 has been subjected to intense scrutiny. Despite considerable interest, knowledge of many aspects of its structure and function remain elusive because of its overall structural complexity and the lack of facile genetic approaches. It is established, however, that eIF3 is involved in both ribosome biogenesis and protein synthesis in eukaryotes (4). Concerted binding of initiation factors is required to initiate protein synthesis and recruit transfer and messenger RNAs to the 40S subunit before assembly of active ribosomes (5). eIF3 binding may take place initially during this process, together with eIF1 and eIF1A to the 40S, followed by binding of the Met-tRNA i -eIF2-GTP complex. Then mRNA binding, scanning, and AUG recognition occur, enabling the 60S subunit to join to form elongation-competent 80S ribosomes (6). An alternative pathway of initiating protein synthesis, often used by viruses, involves a structured sequence in the 5Ј untranslated region of mRNA known as the internal ribosome entry site (IRES). These sequences promote translation initiation without requiring the full complement of eukaryotic initiation factors (5-7). The hepatitis C virus (HCV) IRES is recognized specifically by the small ribosomal subunit and eIF3 before viral translation initiation, forming stable complexes ...
Current challenges in the field of structural genomics point to the need for new tools and technologies for obtaining structures of macromolecular protein complexes. Here, we present an integrative computational method that uses molecular modelling, ion mobility-mass spectrometry (IM-MS) and incomplete atomic structures, usually from X-ray crystallography, to generate models of the subunit architecture of protein complexes. We begin by analyzing protein complexes using IM-MS, and by taking measurements of both intact complexes and sub-complexes that are generated in solution. We then examine available high resolution structural data and use a suite of computational methods to account for missing residues at the subunit and/or domain level. High-order complexes and sub-complexes are then constructed that conform to distance and connectivity constraints imposed by IM-MS data. We illustrate our method by applying it to multimeric protein complexes within the Escherichia coli replisome: the sliding clamp, (β2), the γ complex (γ3δδ′), the DnaB helicase (DnaB6) and the Single-Stranded Binding Protein (SSB4).
Sites of base loss in DNA arise spontaneously, are induced by damaging agents or are generated by DNA glycosylases. Repair of these potentially mutagenic or lethal lesions is carried out by apurinic/apyrimidinic (AP) endonucleases. To test current models of AP site recognition, we examined the effects of site-specific DNA structural modifications and an F266A mutation on incision and protein-DNA complex formation by the major human AP endonuclease, Ape. Changing the ring component of the abasic site from a neutral tetrahydrofuran (F) to a positively charged pyrrolidine had only a 4-fold effect on the binding capacity of Ape. A non-polar 4-methylindole base analog opposite F had a <2-fold effect on the incision activity of Ape and the human protein was unable to incise or specifically bind 'bulged' DNA substrates. Mutant Ape F266A protein complexed with F-containing DNA with only a 6-fold reduced affinity relative to wild-type protein. Similar studies are described using Escherichia coli AP endonucleases, exonuclease III and endonuclease IV. The results, in combination with previous findings, indicate that the ring structure of an AP site, the base opposite an AP site, the conformation of AP-DNA prior to protein binding and the F266 residue of Ape are not critical elements in targeted recognition by AP endonucleases.
In primary biliary cirrhosis (PBC), the major autoepitope recognized by both T and B cells is the inner lipoyl domain of the E2 component of pyruvate dehydrogenase. To address the hypothesis that PBC is induced by xenobiotic exposure, we took advantage of ab initio quantum chemistry and synthesized the inner lipoyl domain of E2 component of pyruvate dehydrogenase, replacing the lipoic acid moiety with synthetic structures designed to mimic a xenobiotically modified lipoyl hapten, and we quantitated the reactivity of these structures with sera from PBC patients. Interestingly, antimitochondrial Abs from all seropositive patients with PBC, but no controls, reacted against 3 of the 18 organic modified autoepitopes significantly better than to the native domain. By structural analysis, the features that correlated with autoantibody binding included synthetic domain peptides with a halide or methyl halide in the meta or para position containing no strong hydrogen bond accepting groups on the phenyl ring of the lysine substituents, and synthetic domain peptides with a relatively low rotation barrier about the linkage bond. Many chemicals including pharmaceuticals and household detergents have the potential to form such halogenated derivatives as metabolites. These data reflect the first time that an organic compound has been shown to serve as a mimeotope for an autoantigen and further provide evidence for a potential mechanism by which environmental organic compounds may cause PBC.
UmuD 2 cleaves and removes its N-terminal 24 amino acids to form UmuD 2 , which activates UmuC for its role in UV-induced mutagenesis in Escherichia coli. Cells with a non-cleavable UmuD exhibit essentially no UV-induced mutagenesis and are hypersensitive to killing by UV light. UmuD binds to the  processivity clamp ("") of the replicative DNA polymerase, pol III. A possible -binding motif has been predicted in the same region of UmuD shown to be important for its interaction with . We performed alanine-scanning mutagenesis of this motif ( 14 TFPLF 18 ) in UmuD and found that it has a moderate influence on UV-induced mutagenesis but is required for the cold-sensitive phenotype caused by elevated levels of wild-type UmuD and UmuC. Surprisingly, the wild-type and the -binding motif variant bind to  with similar K d values as determined by changes in tryptophan fluorescence. However, these data also imply that the single tryptophan in  is in strikingly different environments in the presence of the wild-type versus the variant UmuD proteins, suggesting a distinct change in some aspect of the interaction with little change in its strength. Despite the fact that this novel UmuD variant is non-cleavable, we find that cells harboring it display phenotypes more consistent with the cleaved form UmuD, such as resistance to killing by UV light and failure to exhibit the cold-sensitive phenotype. Cross-linking and chemical modification experiments indicate that the N-terminal arms of the UmuD variant are less likely to be bound to the globular domain than those of the wild-type, which may be the mechanism by which this UmuD variant acts as a UmuD mimic.The umuDC gene products are induced as part of the SOS response and are responsible for much of the UV-induced mutagenesis in Escherichia coli (1). These gene products are subject to an elaborate set of controls that regulate their activity (1). The LexA repressor provides transcriptional control, and there are several proteolytic controls on both the umuD and umuC gene products (1). The homodimeric protein UmuD 2 is the predominant species during the first about 20 -30 min after SOS induction (2). UmuD 2 , together with UmuC, plays a role in a DNA damage checkpoint, decreasing the rate of DNA synthesis and allowing time for accurate repair processes to act (2). This correlates with the cold-sensitive phenotype observed under conditions of overexpression of the umuDC gene products (2, The wealth of structural data and models available for UmuD 2 and UmuDЈ 2 provide insight into how the two forms of the umuD gene products engage in multiple highly specific interactions ( Fig. 1) (4 -8), including with the ␣, , and ⑀ subunits of the replicative polymerase, pol III (9). Of the two forms, UmuD 2 interacts more strongly with the  processivity clamp (also referred to as  or the  clamp) than does UmuDЈ 2 (9, 10). In full-length UmuD 2 , the 39-amino acid N-terminal arms are stably bound to the globular C-terminal domain (4, 7) and form a distinct interaction surface. In t...
Abasic (AP) sites constitute a common form of DNA damage, arising from the spontaneous or enzymatic breakage of the N-glycosyl bond and the loss of a nucleotide base. To examine the effects of such damage on DNA structure, especially in the vicinity of the abasic sugar, four 1.5 ns molecular dynamics simulations of double-helical DNA dodecamers with and without a single abasic (tetrahydrofuran, X) lesion in a 5'-d(CXT) context have been performed and analyzed. The results indicate that the abasic site does not maintain a hole or gap in the DNA, but instead perturbs the canonical structure and induces additional flexibility close to the abasic site. In the apurinic simulations (i.e., when a pyrimidine is opposite the AP site), the abasic sugar flipped in and out of the minor groove, and the gap was water filled, except during the occurrence of a novel non-Watson-Crick C-T base pair across the abasic site. The apyrimidinic gap was not penetrated by water until the abasic sugar flipped out and remained extrahelical. Both AP helices showed kinks of 20-30 degrees at the abasic site. The Watson-Crick hydrogen bonds are more transient throughout the DNA double helices containing an abasic site. The abasic sugar displayed an unusually broad range of sugar puckers centered around the northern pucker. The increased motion of the bases and backbone near the abasic site appear to correlate with sequence-dependent helical stability. The data indicate that abasic DNA contorts more easily and in specific ways relative to unmodified DNA, an aspect likely to be important in abasic site recognition and hydrolysis.
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