Class I Myosins, molecular motors involved in cell migration and cancer

Diaz-Valencia, Juan D.; Estrada-Abreo, Laura A.; Rodríguez-Cruz, Leonor; Salgado-Aguayo, Alfonso; Patiño-López, Genaro

doi:10.1080/19336918.2021.2020705

Cited by 16 publications

(10 citation statements)

References 119 publications

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“…Due to the wide range of noncancerous diseases presented by a woman, the expression of these biomarkers could be helpful for the differentiation between the absence or presence of cancer in early stages. For instance, MYO1G has a special role in cell transport and migration; however, there are no conclusive studies that can relate it to cancer . Additionally, IGLV4-3 appears due to an immune response of the organism, while some studies show that APOC3 can be found in patients with polycystic ovary syndrome .…”

Section: Resultsmentioning

confidence: 99%

“…For instance, MYO1G has a special role in cell transport and migration; however, there are no conclusive studies that can relate it to cancer. 26 Additionally, IGLV4-3 appears due to an immune response of the organism, while some studies show that APOC3 can be found in patients with polycystic ovary syndrome. 27 On the other hand, Figure 4e also identifies the presence of MUC1 and SRGN significantly elevated in EVs from early stage HGSC donors compared to BC donors.…”

Section: High Asymmetrical Field Ion Mobility (Faims) Coupled To Line...mentioning

confidence: 99%

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Tandem SERS and MS/MS Profiling of Plasma Extracellular Vesicles for Early Ovarian Cancer Biomarker Discovery

Veliz,

Cooper,

Grenier-Pleau

et al. 2024

ACS Sens.

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Extracellular vesicles (EVs) are vectors of biomolecular cargo that play essential roles in intercellular communication across a range of cells. Protein, lipid, and nucleic acid cargo harbored within EVs may serve as biomarkers at all stages of disease; however, the choice of methodology may challenge the specificity and reproducibility of discovery. To address these challenges, the integration of rigorous EV purification methods, cutting-edge spectroscopic technologies, and data analysis are critical to uncover diagnostic signatures of disease. Herein, we demonstrate an EV isolation and analysis pipeline using surface-enhanced Raman spectroscopy (SERS) and mass spectrometry (MS) techniques on plasma samples obtained from umbilical cord blood, healthy donor (HD) plasma, and plasma from women with early stage high-grade serous carcinoma (HGSC). Plasma EVs were purified by size exclusion chromatography and analyzed by surface-enhanced Raman spectroscopy (SERS), mass spectrometry (MS), and atomic force microscopy. After determining the fraction of highest EV purity, SERS and MS were used to characterize EVs from HDs, pooled donors with noncancerous gynecological ailments (n = 6), and donors with early stage [FIGO (I/II)] with HGSC. SERS spectra were subjected to different machine learning algorithms such as PCA, logistic regression, support vector machine, nai ̈ve Bayes, random forest, neural network, and k nearest neighbors to differentiate healthy, benign, and HGSC EVs. Collectively, we demonstrate a reproducible workflow with the potential to serve as a diagnostic platform for HGSC.

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Section: Resultsmentioning

confidence: 99%

Section: High Asymmetrical Field Ion Mobility (Faims) Coupled To Line...mentioning

confidence: 99%

Tandem SERS and MS/MS Profiling of Plasma Extracellular Vesicles for Early Ovarian Cancer Biomarker Discovery

Veliz,

Cooper,

Grenier-Pleau

et al. 2024

ACS Sens.

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“…Our findings demonstrated that myosin1a colocalized with F‐actin in mature osteoclasts and its expression level was downregulated by Phi, as well as RhoA/ROCK1 inhibitors (Figure 8d). Class I myosins are a family of motors attached to actin filaments that function in various cellular processes such as the formation of cell surface projections, endocytosis, exocytosis, phagocytosis, cytoskeleton plasticity regulation, membrane tension, and cell migration (Diaz‐Valencia et al, 2022; Tyska & Nambiar, 2010). Additionally, in enterocytes, myosin1a was demonstrated to be a critical component responsible for forming highly ordered structures of the brush border, which were necessary for maintaining normal functions like nutrient absorption and digestive enzyme secretion (Tyska et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Phillygenin prevents osteoclast differentiation and bone loss by targeting RhoA

Zhang,

Jiang,

Zhang

et al. 2024

Phytotherapy Research

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Forsythia suspensa tea is a popular traditional Chinese medicine decoction for its healthy and therapeutic benefits. However, its effects in bone metabolism were not clear. In recent study, we uncovered anti‐osteoclastogenesis property of Phillygenin (Phi), a compound abundant in Forsythia suspensa leaves, and aimed to investigate the effect and mechanism of Phi on bone metabolism in vivo and in vitro. Lipopolysaccharides‐induced murine calvaria osteolysis and ovariectomy‐induced bone loss animal models were used to identify the bone‐protective effect of Phi in vivo and micro‐CT, pQCT, and TRAP staining were applied. We used CCK8, TUNEL, BrdU, and TRAP staining to evaluate the efficacy of Phi on the proliferation and formation of OCs in primary mBMMs. RNA sequence, activity‐based protein profiling, molecular docking, G‐LISA, and WB were used to inspect the target and underlying mechanism of Phi's actions in mBMMs. We found Phi significantly inhibited bone resorption in vivo and inhibited mBMMs osteoclastogenesis in vitro. Ras homolog gene family member A (RhoA) was identified as the direct target of Phi. It counteracted the effects of RhoA activator and acted as a RhoA inhibitor. By targeting RhoA, Phi modulated Rho‐associated coiled‐coil containing protein kinase 1 (ROCK1) activity and regulated its downstream NF‐κB/NFATc1/c‐fos pathway. Furthermore, Phi depressed the disassembling of F‐actin ring through cofilin and myosin1a. Our findings provided Phi as a potential option for treating bone loss diseases by targeting RhoA and highlighted the importance of F. suspensa as a preventive approach in bone disorders.

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“…They are also the second most extensive family of myosins (after class II myosins), comprising eight members (MYO1A‐H) in humans. Most type I myosins exhibit widespread expression, including MYO1B, 1C, 1D, and 1E (Bähler, Kroschewski, Stöffler, & Behrmann, 1994; Bement, Wirth, & Mooseker, 1994; Ruppert, Kroschewski, & Bähler, 1993; Sielski, Ihnatovych, Hagen, & Hofmann, 2014), while others are predominantly expressed in some tissues or cell types, such as MYO1A in the intestinal epithelium (Skowron, Bement, & Mooseker, 1998) and MYO1F and 1G in cells of the immune system (Diaz‐Valencia, Estrada‐Abreo, Rodríguez‐Cruz, Salgado‐Aguayo, & Patiño‐López, 2022; Kim et al, 2006; Pierce et al, 2001).…”

Section: Myosin‐imentioning

confidence: 99%

Myosin motors on the pathway of viral infections

2022

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Molecular motors are microscopic machines that use energy from adenosine triphosphate (ATP) hydrolysis to generate movement. While kinesins and dynein are molecular motors associated with microtubule tracks, myosins bind to and move on actin filaments. Mammalian cells express several myosin motors. They power cellular processes such as endo‐ and exocytosis, intracellular trafficking, transcription, migration, and cytokinesis. As viruses navigate through cells, they may take advantage or be hindered by host components and machinery, including the cytoskeleton. This review delves into myosins' cell roles and compares them to their reported functions in viral infections. In most cases, the previously described myosin functions align with their reported role in viral infections, although not in all cases. This opens the possibility that knowledge obtained from studying myosins in viral infections might shed light on new physiological roles for myosins in cells. However, given the high number of myosins expressed and the variety of viruses investigated in the different studies, it is challenging to infer whether the interactions found are specific to a single virus or can be applied to other viruses with the same characteristics. We conclude that the participation of myosins in viral cycles is still a largely unexplored area, especially concerning unconventional myosins.

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Class I Myosins, molecular motors involved in cell migration and cancer

Cited by 16 publications

References 119 publications

Tandem SERS and MS/MS Profiling of Plasma Extracellular Vesicles for Early Ovarian Cancer Biomarker Discovery

Tandem SERS and MS/MS Profiling of Plasma Extracellular Vesicles for Early Ovarian Cancer Biomarker Discovery

Phillygenin prevents osteoclast differentiation and bone loss by targeting RhoA

Myosin motors on the pathway of viral infections

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