Class B trichothecene chemotyping in Fusarium species by PCR assay

Yörük, Emre; Gazdağlı, Aylin; Albayrak, Gülruh

doi:10.2298/gensr1403661y

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…In the same way, Yörük and Albayrak (2012) described 12 fungal isolates as F. graminearum with a UBC85 primer pair, while 20 were identified as F. culmorum with OPT18 primers (20). In another study, Yörük et al ( 2014) described eight F. graminearum and four F. culmorum isolates among 17 fungal samples by targeting the same SCAR regions (21). Moreover, Abedi-Tizaki and Sabbagh (2012) identified 51 F. culmorum isolates among 344 studied specimens by using the OPT18 primer pair for amplification of the SCAR marker (31).…”

Section: Resultsmentioning

confidence: 98%

“…By considering all these risks, chemotyping, carried out by chemical profile determination of distinct mycotoxins, which are represented by a family or group of related preliminary compounds, is a requirement for the detection of effective methods to cope with the diseases directly caused by pathogens and toxication on animals or humans caused by its natural metabolites. It also has great importance in the characterization of phytopathogenic fungi (20,21).…”

Section: Introductionmentioning

confidence: 99%

“…Hence, molecular approaches along with morphological characterization are effectively used in this field, as they provide rapid access to delicate and accurate findings for the identification of species. The amplification of a targeted definite genomic region also known as genotyping, nucleic acid sequencing both any specific regions or the whole genome and chemotyping carried out by using DNA amplification methods or chromatographic-based methods are efficiently used for classification and characterization of Fusarium species (2,21,23).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods

Yüksektepe¹,

Sefer²,

Varol³

et al. 2022

ejb

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Objective: Fusarium spp. cause Fusarium head blight (FHB) and crown rot (CR) diseases. They also have harmful effects on animal and human health through their mycotoxins. Within the scope of this study, F. graminearum and F. culmorum isolates were purified from wheat ears and stalks contaminated with phytopathogens, which had been collected from various regions of Turkey, were identified and characterized by conventional and molecular methods. Materials and Methods:Sixty-eight Fusarium samples were isolated by single spore analysis and classified according to their macroconidia shape and size. Morphologically characterized samples were verified by amplification of SCAR markers. Their mating types (MAT) and chemotypes were also determined through polymerase chain reaction (PCR).Results: Thirty-eight F. graminearum and 30 F. culmorum isolates were identified via amplification of UBC85 and OPT18 SCAR markers, respectively. All isolates were determined as trichothecene producers by amplification of the tri5 gene. All F. graminarum isolates carry both MAT-1 and MAT-2 loci, whereas 7 of F. culmorum isolates were also determined as MAT-1 and 23 of them as MAT-2 mating types. Deoxynivalenol production capacity of all isolates was identified by tri13 amplification for chemotype determination. Conclusion:Routine monitoring of phytopathogens and their mycotoxin levels is a requirement since their annual levels may vary depending on environmental factors. This work provides knowledge about the distribution of Fusarium spp. leading to FHB and CR in different regions of Turkey between 2010 and 2020. Also, their chemotypes were demonstrated. Our studies will contribute to disease profiling and it is the first step in disease management.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%