CK1 inhibitor affects in vitro maturation and developmental competence of bovine oocytes

Shi, Pengfei; Xu, Jie; Zhao, Xin; Shen, Penglei; Wen, Dongmei; Yu, Qing; Deng, Yanfei; Shi, Dongquan; Lu, Fenghua

doi:10.1111/rda.13483

Cited by 7 publications

(7 citation statements)

References 46 publications

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“…Moreover, the organization of linear molecules of hyaluronan is mediated by TNFAIP6 [33] and PTX-3 [34]. It has been reported that these factors were involved in the quality of oocyte maturation [35]. In the present study, supplementation of the IVM medium with 10 µg/mL TA significantly increased the cumulus expansion of porcine COCs and the expression of genes related to cumulus expansion in cumulus cells.…”

Section: Discussionsupporting

confidence: 57%

Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs

et al. 2021

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To investigate the effects of tannins (TA) on porcine oocyte in vitro maturation (IVM), different concentrations of TA (0, 1, 10 and 100 μg/mL) were supplemented with a maturation medium and the COCs and subsequent embryonic development were examined. The results showed that 10 µg/mL TA significantly improved the cumulus expansion index (CEI), cumulus-expansion-related genes (PTGS1, PTGS2, PTX-3, TNFAIP6 and HAS2) expression and blastocyst formation rates after parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) compared to the control groups, but not oocyte nuclear maturation. Nevertheless, 10 µg/mL TA dramatically enhanced the mRNA expression of oocyte-development-related genes (BMP15, GDF9, CDC2 and CYCLIN B1), GSH, ATP, SOD1, PGC1α, BMP15, GDF9 and CDC2 levels and reduced intracellular ROS level in porcine oocytes. These results indicated that porcine oocyte cytoplasmic maturation was improved by 10 µg/mL TA treatment during IVM. In contrast, a high concentration of TA (100 μg/mL) significantly decreased the CEI and PTGS1, PTGS2, PTX-3 and HAS2 mRNA expressions in cumulus cells, and reduced oocyte nuclear maturation and the total cell numbers/blastocyst. In general, these data showed that 10 μg/mL TA supplementation has beneficial effects on oocyte cytoplasmic maturation and subsequent embryonic development in pigs.

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Section: Discussionsupporting

confidence: 57%

Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs

et al. 2021

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“…Using GAPDH as an internal reference, the relative expression levels were calculated by the 2 −∆∆Ct method. Total RNA extraction, reverse transcription and real-time PCR were reference to a report [ 32 ]. Related primers are shown in Supplementary Table S1 .…”

Section: Methodsmentioning

confidence: 99%

RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway

Shi

Xia

et al. 2022

IJMS

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Melanoma originates from the malignant transformation of melanocytes. Compared with other skin cancers, melanoma has a higher fatality rate. The 5-year survival rate of patients with early-stage primary melanoma through surgical resection can reach more than 90%. However, the 5-year survival rate of patients with metastatic melanoma is only 25%. Therefore, accurate assessment of melanoma progression is critical. Previous studies have found that Retinoic Acid Induced 14(RAI14) is critical in tumorigenesis. However, the biological function of RAI14 for the development of melanoma is unclear. In this study, RAI14 is highly expressed in melanoma and correlated with prognosis. The expression of RAI14 can affect the proliferation, migration and invasion of melanoma cells. F-Box Protein 32(FBXO32) is an E3 ubiquitin ligase of c-MYC. We found that RAI14 affects the transcriptional expression of FBXO32 and regulates the stability of c-MYC. These results suggest that RAI14 play an important role in the growth of melanoma and is expected to be a therapeutic target for melanoma.

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“…Approximately one thousand COCs were collected. The full expansion of CCs was recorded as three points; the partial expansion was recorded as two points; and no extension was recorded as one point (Shi et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

PS48 promotes in vitro maturation and developmental competence of porcine oocytes through activating PI3K/Akt signalling pathway

Jiao

Zhu

et al. 2020

Reprod Domestic Animals

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The quality of oocytes is closely associated with the successful production of porcine oocytes in vitro. However, the regulating mechanisms of oocytes in vitro maturation (IVM) need to be further improved, and the quality of oocyte maturation in vitro is still inferior to that of oocyte maturation in vivo. Compared with oocytes matured in vivo, the oocytes matured in vitro tend to have such problems as more high precision fertilization rate, low blastocyst formation rate, not fully unified oocyte cytoplasm and nuclei of mature in the current maturation systems (Jiao et al., 2020). Moreover, the mature quality of porcine oocytes has an essential effect on embryo development after in vitro fertilization (IVF), nuclear transfer (NT) and parthenogenetic activation (PA) (Kim et al., 2010). Oocyte

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CK1 inhibitor affects in vitro maturation and developmental competence of bovine oocytes

Cited by 7 publications

References 46 publications

Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs

Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs

RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway

PS48 promotes in vitro maturation and developmental competence of porcine oocytes through activating PI3K/Akt signalling pathway

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