Citrus sinensis a potential solution against superbugs

Fatima, Tehreem; Mian, Abrar Hussain; Khan, Zuhair S.; Khan, Ahmad; Anwar, Faheem; Tariq, Adnan; Sardar, Momina

doi:10.1007/s13204-020-01408-9

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…The growth of these trees under Egyptian conditions is very suitable and therefore economical in use. Further, the extracts from these trees were shown potential activities against the growth of bacteria and fungi (Nair and Chanda, 2007 ; Ekwenye and Edeha, 2010 ; El-Hefny et al, 2017 ; Bhawana et al, 2018 ; Salem et al, 2019 ; Abdelkhalek et al, 2020 ; Abo-Elgat et al, 2020 ; Afzal et al, 2020 ; Fatima et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic Pigments and Biochemical Response of Zucchini (Cucurbita pepo L.) to Plant-Derived Extracts, Microbial, and Potassium Silicate as Biostimulants Under Greenhouse Conditions

Abd-Elkader

Mohamed²,

Feleafel

et al. 2022

Front. Plant Sci.

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There are many technological innovations in the field of agriculture to improve the sustainability of farmed products by reducing the chemicals used. Uses of biostimulants such as plant extracts or microorganisms are a promising process that increases plant growth and the efficient use of available soil resources. To determine the effects of some biostimulants' treatments on the photosynthetic pigments and biochemicals composition of zucchini plants, two experiments were conducted in 2019 and 2020 under greenhouse conditions. In this work, the effects of beneficial microbes (Trichoderma viride and Pseudomonas fluorescens), as well as three extracts from Eucalyptus camaldulensis leaf extract (LE), Citrus sinensis LE, and Ficus benghalensis fruit extract (FE) with potassium silicate (K2SiO3) on productivity and biochemical composition of zucchini fruits, were assessed as biostimulants. The results showed that E. camaldulensis LE (4,000 mg/L) + K2SiO3 (500 mg/L) and T. viride (106 spore/ml) + K2SiO3 (500 mg/L) gave the highest significance yield of zucchini fruits. Furthermore, the total reading response of chlorophylls and carotenoids was significantly affected by biostimulants' treatments. The combination of K2SiO3 with E. camaldulensis LE increased the DPPH scavenging activity and the total phenolic content of zucchini fruits, in both experiments. However, the spraying with K2SiO3 did not observe any effects on the total flavonoid content of zucchini fruits. Several phenolic compounds were identified via high-performance liquid chromatography (HPLC) from the methanol extracts of zucchini fruits such as syringic acid, eugenol, caffeic acid, pyrogallol, gallic acid, ascorbic acid, ferulic acid, α-tocopherol, and ellagic acid. The main elemental content (C and O) analyzed via energy-dispersive X-ray spectroscopy (EDX) of leaves was affected by the application of biostimulants. The success of this work could lead to the development of cheap and easily available safe biostimulants for enhancing the productivity and biochemical of zucchini plants.

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Section: Introductionmentioning

confidence: 99%

Photosynthetic Pigments and Biochemical Response of Zucchini (Cucurbita pepo L.) to Plant-Derived Extracts, Microbial, and Potassium Silicate as Biostimulants Under Greenhouse Conditions

Abd-Elkader

Mohamed²,

Feleafel

et al. 2022

Front. Plant Sci.

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“…The pH of the medium was adjusted by using 10NH 2 SO 4 and 10N NaOH. The media was sterilized and then placed in incubator for about 24 hours to check contamination (Tehreem et al, 2020).…”

Section: Speci Cationmentioning

confidence: 99%

Simultaneous Removal of Iron and Hexavalent Chromium Via Slow Sand Filter Inoculated With Thiobacillus ferrooxidans and Waste management

Mian¹

2021

Preprint

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Many places have common physical, biological, and chemical hazards and heavy metals contamination in drinking water is very important to study especially near industrial areas and mining sources. Application of microbes along with other physicochemical techniques can prove to be the suitable remediation technology for the removal of metals from water. This study aimed to remove iron and hexavalent chromium from water using slow sand filters inoculated with iron-oxidizing bacteria Acidothiobacillus ferrooxidans. An experiment was performed for 37 days under controlled conditions, a water sample was prepared synthetically and another sample was collected from water filtration plant from Abbottabad city in KP Province- Pakistan and influents and effluents were tested for different parameters like pH, turbidity, hardness, total plate count, total dissolved solids, electrical conductivity and analysis of iron and hexavalent chromium in water. Acidothiobacillus ferrooxidans culture was isolated from soil using the differential iron media i.e. 9K medium and was inoculated into sand filters. At the end of the experiment, the removal efficiency of ferrous iron was about 52% and that of hexavalent chromium was 60.2%. In simultaneous treatment, it was observed that removal efficiency of iron was about 68% while that of hexavalent chromium was about 93% suggesting that slow sand filters were more effective for the removal of hexavalent chromium than iron. Hence, the designed slow sand filters were able to reduce the concentration of hexavalent chromium more efficiently than ferrous iron. And it was observed that slow sand filters can effectively remove ferrous iron and hexavalent chromium from water if they are inoculated with respective bacteria. Key,words,. Hexavalent Chromium, Iron Oxidizing, Sand Filters, Acidothiobacillus ferrooxidans, waste management.

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Molecular detection of Shiga toxin-producing Escherichia coli (STEC) O157 in sheep, goats, cows and buffaloes

Ullah

Irshad²,

Ahmed

et al. 2021

Mol Biol Rep

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A total of 400 Recto-anal mucosal swabs samples (200 from each region) were collected and processed initially by conventional PCR. Among them, only two (n=2) RAMS samples were detected and positive for E. coli O157 gene, included one in RAMS samples of sheep from slaughterhouse of Rawalpindi, while another in RAMS samples of buffalos from slaughterhouse of Islamabad. The rest of the samples from goats and cows showed negative results for E. coli O157 in both regions. E. coli O157 isolates were obtained from each positive sample onto two different Sorbitol MacConkey Agar media plates and analyzed again through conventional PCR, resulted only single 1/6 isolated colony con rmed from both samples which possessed rfbE genes. The overall prevalence of rfbE (O157) genes came up to (0.5%). Furthermore, for the presence of STEC other virulent genes (sxt1, stx2, eae and ehlyA), these both positive rfbE isolated colonies were then brie y subjected towards multiplex PCR. Results revealed that none of the STEC virulent genes were detected in single (01) positive rfbE O157 isolated colony of sheep sample. While fortunately 4 STEC virulent genes (sxt1, stx2, eae and ehlyA) were detected in single (01) positive rfbE O157 isolated colony of buffalo sample. It was concluded that healthy adult sheep and buffalo are possibly essential carriers of STEC O157. However, buffalo sample possessed 4 STEC virulent genes, hence considered an important source of STEC infection to humans either through direct or indirect transmission which should devise proper control systems.

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Citrus sinensis a potential solution against superbugs

Cited by 6 publications

References 16 publications

Photosynthetic Pigments and Biochemical Response of Zucchini (Cucurbita pepo L.) to Plant-Derived Extracts, Microbial, and Potassium Silicate as Biostimulants Under Greenhouse Conditions

Photosynthetic Pigments and Biochemical Response of Zucchini (Cucurbita pepo L.) to Plant-Derived Extracts, Microbial, and Potassium Silicate as Biostimulants Under Greenhouse Conditions

Simultaneous Removal of Iron and Hexavalent Chromium Via Slow Sand Filter Inoculated With Thiobacillus ferrooxidans and Waste management

Molecular detection of Shiga toxin-producing Escherichia coli (STEC) O157 in sheep, goats, cows and buffaloes

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