Citrus, Milk Thistle, and Propolis Extracts Improved the Intestinal Permeability of Curcuminoids from Turmeric Extract─ an In Silico and In Vitro Permeability Caco-2 Cells Approach

Abstract

Curcumin has been traditionally used in food recipes for biological health benefits. However, these properties are compromised due to its very low bioavailability. Thus, this work assessed the impact of using natural potential bioenhancers to improve intestinal permeability and understanding the interaction of flavonoids with curcuminoids through molecular dynamic simulation. The intestinal permeability (P app) was measured on the human colon carcinoma cell line (Caco-2). The P app values measured on Caco-2 cell monolayers provided information regarding the increased permeability of curcumin across the intestinal barrier specially caused by the presence of citrus (100.45%), and milk thistle extracts (81.70%) at level +1 showed the higher P app increase (%) compared to level 0. With respect to the soft standardized green propolis EPP-AF extract, the P app increase was higher at level 0 (26.34%). Molecular dynamic (MD) simulations of curcumin with naringin and silybin B confirmed this increase of permeability of curcumin at higher concentrations through non-covalent interactions. These results suggest the prominent importance of the association of natural flavonoids as bioenhancers to increase curcumin bioavailability and health benefits.

Role of Analytical and Organic Chemistry Supporting the Challenges in Food Preservation and Safety

“…Different bioenhancers (citrus fruit extract, milk thistle extract, and green propolis extract) were used to improve the intestinal permeability of curcuminoids using a human colon carcinoma cell line. 9 Molecular dynamics simulations confirmed the positive effect of naringin and silybin B through noncovalent interactions. By examining innovative approaches for future agriculture, the review focused on different aspects of Brassicaceae microgreens to identify an interesting approach to ensure their production because they are a source of healthpromoting phytochemicals.…”

“…Curcuminoids exhibit many health-promoting properties, but in brief, their bioavailability is low. Different bioenhancers (citrus fruit extract, milk thistle extract, and green propolis extract) were used to improve the intestinal permeability of curcuminoids using a human colon carcinoma cell line . Molecular dynamics simulations confirmed the positive effect of naringin and silybin B through noncovalent interactions.…”

Enhanced absorption of prenylated cinnamic acid derivatives from Brazilian green propolis by turmeric in humans and rats

“…Several studies have revealed enhanced absorption of herbal constituents due to the co‐administration of several herbs (Sun et al., 2019 ). Recently, green propolis has been reported in vitro to improve the intestinal membrane permeability of curcuminoids (de Albuquerque et al., 2023 ), suggesting interaction between components of natural products, thereby affecting their absorption. Therefore, the absorption of BGP constituents is affected by their interaction with other herbs.…”