Citrus huanglongbing in São Paulo State, Brazil: PCR detection of the ‘Candidatus’ Liberibacter species associated with the disease

Teixeira, Diva do Carmo; Danet, Jean-Luc; Eveillard, Sandrine; Martins, E. C.; Jesús, Waldir Cintra de; Yamamoto, Pedro Takao; Lopes, S. A.; Bassanezi, R. B.; Ayres, A. J.; Saillard, Colette; Bové, Joseph M.

doi:10.1016/j.mcp.2004.11.002

Cited by 219 publications

(67 citation statements)

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“…There are only a few publications that analyzed the bacterial communities in citrus samples by PCR methods. Most of the work was done on fruit pathogen identification on strains such as Candidatus liberibacter by PCR [23][24][25] or real-time PCR [26], Methylobacterium mesophilicum [27], Xanthomonas axonopodis pv. citri [28] and Xylella fastidiosa [29] but no references relate to the DGGE method.…”

Section: Discussionmentioning

confidence: 99%

Determination of citrus fruit origin by using 16S rDNA fingerprinting of bacterial communities by PCR- DGGE: an application to clementine from Morocco and Spain

Nguyen¹,

Gemrot²,

Loiseau³

et al. 2008

Fruits

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--Introduction. Consumption of citrus fruits and their juice has strongly grown in the last few decades. However, at the present time, the traceability of these fruits is only documentary. In case of doubt or fraud, no standardized analysis makes it possible to discriminate or determine the geographical origin of culture of the fruit. Materials and methods. A method of bacterial ecology, PCR-DGGE, was used to characterize the bacterial flora of clementines imported into France from Spain and Morocco in order to show that there is a relation between the bacterial communities of the fruits and their geographical origins. The principle rests on the determination of specific biological markers for a given region. Protocols of microbial extraction and DNA amplification were optimized. Results. DGGE profiles analyzed by multivariate analysis permitted us to distinguish microbial communities from different origins. Conclusion. We propose the PCR-DGGE method as a new tool for traceability that provides citrus products with a unique bar code and makes it possible to trace back the citrus fruit to their original location. Résumé --introduction. La consommation des agrumes et de leur jus s'est fortement déve-loppée pendant ces dernières décennies. Cependant, à l'heure actuelle, la traçabilité de cette filière est uniquement documentaire. En cas de doute ou de fraude, aucune analyse normalisée ne permet de discriminer ou de déterminer l'origine géographique de la culture du fruit.

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Section: Discussionmentioning

confidence: 99%

Determination of citrus fruit origin by using 16S rDNA fingerprinting of bacterial communities by PCR- DGGE: an application to clementine from Morocco and Spain

Nguyen¹,

Gemrot²,

Loiseau³

et al. 2008

Fruits

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“…The first is based on 16S rDNA sequence, using many sequences of primers and probes (Fujikawa and Iwanami 2012). Primer pair OI1 and OI2c is able to amplify the rDNA Las and Laf species (Teixeira et al 2005a). When the 2 Ca.…”

Section: Molecular Techniques For Hlb Detectionmentioning

confidence: 99%

“…Sequence analysis shows that the 16S rDNA amplified from Las has one XbaI restriction site and yields, upon XbaI treatment, 2 fragments of sizes 520 and 640 bp. The 16S rDNA amplified from Laf has an additional restriction site and yields 3 fragments of 520, 506, and 130 bp (Jagoueix et al 1996;Teixeira et al 2005a). The second PCR is centered on the nusG-rplK operon region (primer pair A2 and J5, and primer pair MHO353 and MHO354) (Fujikawa and Iwanami 2012).…”

Section: Molecular Techniques For Hlb Detectionmentioning

confidence: 99%

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A review of techniques for detecting Huanglongbing (greening) in citrus

et al. 2016

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Huanglongbing (HLB) is the most destructive disease of citrus worldwide. Monitoring of health and detection of diseases in trees is critical for sustainable agriculture. HLB symptoms are virtually the same wherever the disease occurs. The disease is caused by Candidatus Liberibacter spp., vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Electron microscopy was the first technique used for HLB detection. Nowadays, scientists are working on the development of new techniques for a rapid HLB detection, as there is no sensor commercially accessible for real-time assessment of health conditions in trees. Currently, the most widely used mechanism for monitoring HLB is exploration, which is an expensive, labor-intensive, and time-consuming process. Molecular techniques such as polymerase chain reaction are used for the identification of HLB disease, which requires detailed sampling and processing procedures. Furthermore, investigations are ongoing in spectroscopic and imaging techniques, profiling of plant volatile organic compounds, and isothermal amplification. This study recognizes the need for developing a rapid, cost-effective, and reliable health-monitoring sensor that would facilitate advancements in HLB disease detection. This paper compares the benefits and limitations of these potential methods for HLB detection.Key words: HLB, detection, trees, monitoring, health.Résumé : La maladie du dragon jaune (MDJ) est la maladie des agrumes la plus destructrice au monde. Pour assurer la durabilité agricole, il est essentiel de contrôler la santé et la présence de maladies chez les arbres. Les symptômes de la MDJ sont pratiquement les mêmes quel que soit le point d'origine de la maladie. Celle-ci est causée par Candidatus Liberibacter spp. et transmise par les psylles Diaphorina citri Kuwayama et Trioza erytreae. La technique de microscopie électronique fut le premier mode de détection de la MDJ. De nos jours, des scientifiques travaillent à l'élaboration de nouvelles techniques de détection de la MDJ puisqu'il n'existe aucun détecteur commercial permettant l'évaluation de l'état de santé des arbres en temps réel. Actuellement, on doit la plupart du temps recourir à l'exploration pour surveiller la MDJ, un processus laborieux, coûteux et pénible. On a recours à des techniques moléculaires comme la réaction de la polymérase en chaîne pour identifier la MDJ, ce qui exige un prélèvement et un traitement minutieux. Par ailleurs, on évalue présentement des techniques de spectroscopie et d'imagerie, le profilage de composés végétaux organiques volatils et l'amplification isothermique. La présente étude met en relief le besoin de mettre au point un dispositif d'analyse de l'état de santé qui serait à la fois rapide, rentable et fiable et qui permettrait de faire progresser la détection de la MDJ. Cet article compare les avantages et les limites de ces méthodes aspirant à la détection de la MDJ. [Traduit par la Rédaction]

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“…Cirus huanglongbing (HLB) is the destructive a-proteobacteria disease of citrus in Asia, Africa, Arabic Peninsular, Mauritius Island, Madagascar (Garnier and Bove,1996) and recently in America and Brazil (Teixeira et al, 2005). Being an important disease, it affects production of citrus in several parts of Malaysia including Terengganu, which is the major producer area of citrus in Malaysia.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Citrus Susceptibility towards Candidatus Liberibacter Asiaticus-Terengganu Isolate Based on Vector and Graft Transmission Tests

Ahmad

Sijam²,

Habibuddin³

et al. 2011

JAS

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Five citrus species namely Citrus reticulata, C. aurantium, C. grandis, C. madurensis and Murraya paniculata were assessed for tolerance by grafting and vector transmission tests toward Ca. L. asiaticus-Terengganu isolate. This study was conducted in an insect-proof glasshouse for a period of six months. Results of this study showed that seedlings of M. paniculata, and C. grandis showed no HLB symptoms six months after inoculation period. They were also showed negative results by polymerase chain reaction (PCR) test and recorded the lowest percentage of reduction rate of plant height and plant biomass. Based on our findings, proved that these two citrus species were resistance to huanglongbing (HLB) or citrus greening infection. However, C. aurantium was showed high tolerance to HLB infection with the trees exhibited mild leaf mottling and green vein and the infected seedlings recovered after 3-4 months after treated with fertilizer. However the pathogen was detected by PCR test in the recovered seedlings. C. reticulata and C. madurensis were very susceptible to HLB infection. Inoculated seedlings showed severe HLB symptoms such as leaf mottling and yellowing, leaf green vein, leaf defoliation, twigs dieback within six months after inoculation. The inoculated seedlings started showing the first symptoms within three months after inoculation. Infected seedlings were also showed positive results with PCR

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Citrus huanglongbing in São Paulo State, Brazil: PCR detection of the ‘Candidatus’ Liberibacter species associated with the disease

Cited by 219 publications

References 4 publications

Determination of citrus fruit origin by using 16S rDNA fingerprinting of bacterial communities by PCR- DGGE: an application to clementine from Morocco and Spain

Determination of citrus fruit origin by using 16S rDNA fingerprinting of bacterial communities by PCR- DGGE: an application to clementine from Morocco and Spain

A review of techniques for detecting Huanglongbing (greening) in citrus

Assessment of Citrus Susceptibility towards Candidatus Liberibacter Asiaticus-Terengganu Isolate Based on Vector and Graft Transmission Tests

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