Citrobacter telavivum sp. nov. with chromosomal mcr-9 from hospitalized patients

Ribeiro, Teresa Gonçalves; Izdebski, Radosław; Urbanowicz, P; Carmeli, Yehuda; Gniadkowski, Marek; Peixe, Luı́sa

doi:10.1007/s10096-020-04003-6

Cited by 29 publications

(26 citation statements)

References 32 publications

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“…Similarly, previous findings detected IncHI2-or IncHI2A-type plasmids in mcr-9 positive Enterobacteriaceae, often together with other resistance determinants (including HPCIAs) and to heavy metals mainly from human patients (Carroll et al, 2019;Chavda et al, 2019;Kieffer et al, 2019;Bitar et al, 2020;Faccone et al, 2020;Kananizadeh et al, 2020;Lin et al, 2020;Osei Sekyere et al, 2020;Soliman et al, 2020;Tsui et al, 2020;Umeda et al, 2020;Elbediwi et al, 2021;Marchetti et al, 2021;Sun et al, 2021), and also from animal (Börjesson et al, 2020;Yuan et al, 2019;Borowiak et al, 2020;Haenni et al, 2020;Leite et al, 2020), food (Borowiak et al, 2020;Sadek et al, 2020;Tyson et al, 2020), and environmental (Kamathewatta et al, 2020) sources. Few reports also described mcr-9 integrated into the chromosome of Citrobacter (Ribeiro et al, 2021) and Salmonella (Pan et al, 2020;Tyson et al, 2020) isolates, with a genetic context similar to the structure observed in mcr-9-harboring plasmid sequences, suggesting a possible mcr-9 transfer as a gene cassette between plasmids and chromosomes (Pan et al, 2020).…”

Section: Discussionmentioning

confidence: 84%

Emergence of IncHI2 Plasmids With Mobilized Colistin Resistance (mcr)-9 Gene in ESBL-Producing, Multidrug-Resistant Salmonella Typhimurium and Its Monophasic Variant ST34 From Food-Producing Animals in Italy

et al. 2021

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A collection of 177 genomes of Salmonella Typhimurium and its monophasic variant isolated in 2014–2019 from Italian poultry/livestock (n = 165) and foodstuff (n = 12), previously screened for antimicrobial susceptibility and assigned to ST34 and single-locus variants, were studied in-depth to check the presence of the novel mcr-9 gene and to investigate their genetic relatedness by whole genome sequencing (WGS). The study of accessory resistance genes revealed the presence of mcr-9.1 in 11 ST34 isolates, displaying elevated colistin minimum inhibitory concentration values up to 2 mg/L and also a multidrug-resistant (MDR) profile toward up to seven antimicrobial classes. Five of them were also extended-spectrum beta-lactamases producers (blaSHV–12 type), mediated by the corresponding antimicrobial resistance (AMR) accessory genes. All mcr-9-positive isolates harbored IncHI2-ST1 plasmids. From the results of the Mash analysis performed on all 177 genomes, the 11 mcr-9-positive isolates fell together in the same subcluster and were all closely related. This subcluster included also two mcr-9-negative isolates, and other eight mcr-9-negative ST34 isolates were present within the same parental branch. All the 21 isolates within this branch presented an IncHI2/2A plasmid and a similar MDR gene pattern. In three representative mcr-9-positive isolates, mcr-9 was demonstrated to be located on different IncHI2/IncHI2A large-size (∼277–297 kb) plasmids, using a combined Illumina–Oxford Nanopore WGS approach. These plasmids were also compared by BLAST analysis with publicly available IncHI2 plasmid sequences harboring mcr-9. In our plasmids, mcr-9 was located in a ∼30-kb region lacking different genetic elements of the typical core structure of mcr-9 cassettes. In this region were also identified different genes involved in heavy metal metabolism. Our results underline how genomics and WGS-based surveillance are increasingly indispensable to achieve better insights into the genetic environment and features of plasmid-mediated AMR, as in the case of such IncHI2 plasmids harboring other MDR genes beside mcr-9, that can be transferred horizontally also to other major Salmonella serovars spreading along the food chain.

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Section: Discussionmentioning

confidence: 84%

Emergence of IncHI2 Plasmids With Mobilized Colistin Resistance (mcr)-9 Gene in ESBL-Producing, Multidrug-Resistant Salmonella Typhimurium and Its Monophasic Variant ST34 From Food-Producing Animals in Italy

et al. 2021

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“…This study confirms their broad host range and ongoing ability to acquire novel resistance determinants, such as carbapenemase genes and mcr-9.1. There appears to be a close association between mcr-9.1 and IncHI2 plasmids, first noted in the initial reports of mcr-9.1 [4,5] and subsequently confirmed in multiple publications on mcr-9.1 [7,8,10,25,26,[28][29][30][34][35][36][37][38][39][40][41][42][43].…”

Section: Discussionmentioning

confidence: 87%

Silent spread of mobile colistin resistance gene mcr-9.1 on IncHI2 ‘superplasmids’ in clinical carbapenem-resistant Enterobacterales

Macesic¹,

Blakeway²,

Stewart³

et al. 2021

Clinical Microbiology and Infection

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…The incompatibility (Inc) type of pAVS0177-a could not be identified, and no similar plasmids (i.e., query coverage of >50%) were found in a BLASTn (MegaBLAST) search of the NCBI nucleotide collection. The 12.5-kb genetic region encompassing mcr-9 (spanning from the transcriptional repressor rncR to the insertion sequence IS 15DII and including IS 903 B directly upstream of mcr-9 ) was structurally identical to the genetic context of mcr-9 described in other species ( 21 , 22 ). In conclusion, we present the complete genome sequence of an mcr -harboring Hafnia isolate and demonstrate that intrinsically resistant species may serve as reservoirs for transferable mcr genes.…”

Section: Announcementmentioning

confidence: 80%

Complete Genome Sequence of Hafnia paralvei Isolate AVS0177, Harboring mcr-9 on a Plasmid

Biggel

Zurfluh

Hoehn³

et al. 2022

Microbiol Resour Announc

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Citrobacter telavivum sp. nov. with chromosomal mcr-9 from hospitalized patients

Cited by 29 publications

References 32 publications

Emergence of IncHI2 Plasmids With Mobilized Colistin Resistance (mcr)-9 Gene in ESBL-Producing, Multidrug-Resistant Salmonella Typhimurium and Its Monophasic Variant ST34 From Food-Producing Animals in Italy

Emergence of IncHI2 Plasmids With Mobilized Colistin Resistance (mcr)-9 Gene in ESBL-Producing, Multidrug-Resistant Salmonella Typhimurium and Its Monophasic Variant ST34 From Food-Producing Animals in Italy

Silent spread of mobile colistin resistance gene mcr-9.1 on IncHI2 ‘superplasmids’ in clinical carbapenem-resistant Enterobacterales

Complete Genome Sequence of Hafnia paralvei Isolate AVS0177, Harboring mcr-9 on a Plasmid

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