Complete Genome Sequence of Hafnia paralvei Isolate AVS0177, Harboring
            <i>mcr-9</i>
            on a Plasmid

Biggel, Michael; Zurfluh, Katrin; Hoehn, Sarah; Schmitt, Kira; Frei, Andrea; Jans, Christoph; Stephan, Roger

doi:10.1128/mra.00966-21

Cited by 3 publications

(4 citation statements)

References 19 publications

(18 reference statements)

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“…None of the H. alvei genomes retrieved from the NCBI database contained mcr -9.1, which suggests the acquisition of mcr -9.1 by H. alvei may be more specific to the U.S. While mcr -9.1 has been reported in the closely related H. paralvei (collected in Lake Zug, Switzerland, 2020), this work marks the first detection of mcr -9.1 within the bacterial species H. alvei . This is the second report of mcr -9.1 within the intrinsically colistin-resistant genus Hafnia , which adds to the growing list of mcr acquisition within intrinsically colistin-resistant bacterial species.…”

Section: Resultsmentioning

confidence: 70%

Emergence of Mobile Colistin Resistance Genes within Los Angeles County Wastewater

Wang

Smith

2023

Environ. Sci. Technol. Lett.

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Wastewater surveillance of antibiotic resistance genes (ARGs) provides insight into the circulating antibiotic-resistant profile of its serviced population. Continual surveillance and characterization of wastewater ARGs could help identify emerging ARGs of clinical importance and their dissemination across bacterial species and locations. In this study, we detected four mobile colistin-resistant (mcr) gene variants (mcr-3.12, mcr-4.3, mcr-5.1, and mcr-9.1) in Los Angeles County wastewater between September 2020 to February 2022. Through sequencing of cultured colistin-resistant bacterial isolates and wastewater plasmids, we characterized the genetic environments of the detected mcr gene variants and revealed conserved mcr gene cassettes between our data set and published data. Moreover, we discovered two novel small plasmids (approximately 8.29 and 8.67 kbp) with near identical mcr-5 gene cassettes flanked by a nonhomologous region. Within cultured colistinresistant bacterial isolates, we detected the presence of mcr-3.12 in Aeromonas hydrophila and mcr-9.1 in Haf nia alvei. Our findings underscore the utility of wastewater surveillance to detect and characterize circulating ARGs and help uncover intermediate carriers along their dissemination routes.

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Section: Resultsmentioning

confidence: 70%

Emergence of Mobile Colistin Resistance Genes within Los Angeles County Wastewater

Wang

Smith

2023

Environ. Sci. Technol. Lett.

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“…With regards to mcr -9 in the environment, there have been reports of its detection in WWTPs in the U.S.A and China (Hassan et al ., 2022; Shi et al ., 2022), hospital effluent in China (Xu et al ., 2021), river water in South Africa (Mbanga et al ., 2021), lake water in Switzerland (Biggel et al ., 2022), sands and seawaters in Brazil (Furlan et al ., 2021), as well as in the source water, influents and effluents of DWTPs in China (Khan et al ., 2021). In addition, following a retrospective analysis, Martiny et al .…”

Section: Discussionmentioning

confidence: 99%

“…With regards to the genetic environment, similar to our findings, many reports to date have indicated the presence of the IS 5 family element IS 903 upstream of the mcr -9 gene, while downstream, they have been generally flanked by IS 1 (IS 1R ) or IS 6 (IS 26 and IS 15DII ) elements (Ai et al ., 2021; Biggel et al ., 2022; Diaconu et al ., 2021; Hendrickx et al ., 2021; Kamathewatta et al ., 2020; Ribeiro et al ., 2021; Tyson et al ., 2020). This highlights the potential involvement of IS 1 , IS 5 and IS 6 family elements in the mobilisation of the mcr -9 gene.…”

Section: Discussionmentioning

confidence: 99%

“…With regards to mcr-9 in the environment, there have been reports of its detection in WWTPs in the U.S.A and China (Hassan et al, 2022;Shi et al, 2022), hospital effluent in China (Xu et al, 2021), river water in South Africa (Mbanga et al, 2021), lake water in Switzerland (Biggel et al, 2022), sands and seawaters in Brazil (Furlan et al, 2021), as well as in the source water, influents and effluents of DWTPs in China (Khan et al, 2021). In addition, following a retrospective analysis, Martiny et al (2022) identified mcr-9 genes in different environmental metagenomes, including those derived from wastewater, air, salt marsh, sludge, subsurface, marine and freshwater environments, which had been uploaded to public databases over the last 10 years.…”

Section: Mcr-9mentioning

confidence: 99%

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First reported detection of the mobile colistin resistance genes,mcr-8 andmcr-9, in the Irish environment

Cahill

Hooban

Fitzhenry

et al. 2022

Preprint

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The emergence and dissemination of mobile colistin resistance (mcr) genes across the globe poses a significant threat to public health, as colistin remains one of the last line treatment options for multi-drug resistant infections. Environmental samples (157 water and 157 wastewater) were collected in Ireland between 2018 and 2020. Samples collected were assessed for the presence of antimicrobial resistant bacteria using Brilliance ESBL, Brilliance CRE, mSuperCARBA and McConkey agar containing a ciprofloxacin disc. All water and integrated constructed wetland influent and effluent samples were filtered and enriched in buffered peptone water prior to culture, while wastewater samples were cultured directly. Isolates collected were identified via MALDI-TOF, were tested for susceptibility to 16 antimicrobials, including colistin, and subsequently underwent whole genome sequencing. Overall, eightmcrpositive Enterobacterales (onemcr-8 and sevenmcr-9) were recovered from six samples (freshwater (n=2), healthcare facility wastewater (n=2), wastewater treatment plant influent (n=1) and integrated constructed wetland influent (piggery farm waste) (n=1)). While themcr-8 positiveK. pneumoniaedisplayed resistance to colistin, all sevenmcr-9 harbouring Enterobacterales remained susceptible. All isolates demonstrated multi-drug resistance and through whole genome sequencing analysis, were found to harbour a wide variety of antimicrobial resistance genes i.e., 30 ± 4.1 (10-61), including the carbapenemases,blaOXA-48 (n=2) andblaNDM-1 (n=1), which were harboured by three of the isolates. Themcrgenes were located on IncHI2, IncFIIK and IncI1-like plasmids. The findings of this study highlight potential sources and reservoirs ofmcrgenes in the environment and illustrate the need for further research to gain a better understanding of the role the environment plays in the persistence and dissemination of antimicrobial resistance.

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First reported detection of the mobile colistin resistance genes, mcr-8 and mcr-9, in the Irish environment

Cahill

Hooban

Fitzhenry

et al. 2023

Science of The Total Environment

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Complete Genome Sequence of Hafnia paralvei Isolate AVS0177, Harboring mcr-9 on a Plasmid

Abstract: Here, we report the complete genome sequence of a Hafnia paralvei strain isolated from a lake in Switzerland in 2020. The genome consists of a 4.7-Mbp chromosome, a large plasmid (213 kb) harboring mcr-9 , and a small plasmid (6 kb).

Cited by 3 publications

References 19 publications

Emergence of Mobile Colistin Resistance Genes within Los Angeles County Wastewater

Emergence of Mobile Colistin Resistance Genes within Los Angeles County Wastewater

First reported detection of the mobile colistin resistance genes,mcr-8 andmcr-9, in the Irish environment

First reported detection of the mobile colistin resistance genes, mcr-8 and mcr-9, in the Irish environment

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