Citric acid production from cellobiose by 2-deoxyglucose-resistant mutant strains of Aspergillus niger in semi-solid culture

Sarangbin, Somsak; Kirimura, Kohtaro; Usami, Shoji

doi:10.1007/bf00170367

Cited by 14 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 Citric acid production by parental strains, A. niger Yang no.2, and T. viride M5S51, as compared to hybrid trains, AT 11-2-3, AT 11-2-4, and AT 11-2-10, using various carbon sources in semisolid culture presence of 1.0 g/l 2-deoxy-D-glucose (DG; data not shown). This result correlated with the previous studies indicating that DG-resistant mutants showed increased citric acid production using glucose and cellobiose as carbon sources [33][34][35].…”

Section: -Deoxy-d-glucose-resistant Propertiessupporting

confidence: 94%

“…With the aim of breeding new fungi that produce citric acid from cellulolytic materials, 2-deoxyglucose-resistant mutant strains of A. niger were induced, showing 49.6 g/l citric acid from 100 g/l cellobiose in semisolid culture [26]. In other trials, the intergeneric protoplast fusion between auxotrophic mutant strains of A. niger and T. viride known as cellulase producer were also carried out [23,24].…”

Section: Introductionmentioning

confidence: 99%

“…Although, there is a high demand of citric acid used each year, it is provided at a low price as a commodity chemical. Therefore, it is necessary to establish a method for citric acid production from inexpensive and readily available raw materials in industrial processes [3][4][5][6][7][8] to achieve technical and economical benefits.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polyploid Formation Between Aspergillus niger and Trichoderma viride For Enhanced Citric Acid Production From Cellulose

Watanapokasin

Sawasjirakij

Usami

et al. 2007

Appl Biochem Biotechnol

Self Cite

View full text Add to dashboard Cite

The first-stage heterokaryons, obtaining from intergeneric protoplast fusion between Aspergillus niger (Y-b) and Trichoderma viride (M5S51), showed slow growth and mixed morphologies on minimal medium. The fusants were classified into heterokaryon and prototrophic haploid, showing the morphology as that of A. niger. The heterokaryon strains formed conidia with the same nutritional requirements as those of the original auxotrophic mutant strains. After several subcultivations on minimal medium containing d-camphor, some heterokaryon strains formed larger two to seven nuclei/conidium as compared to one nucleus/conidium of the auxotrophic mutant and prototrophic strains, indicating that the new hybrids were generated. Interestingly, three fusant strains AT 11-2-3, AT 11-2-10, and AT 11-2-14 produce 19.2, 6.1, and 10.5 g/l citric acid, respectively, in semisolid culture containing cellulose, whereas A. niger Yang no. 2 could not use carboxymethyl cellulose as the sole carbon source for citric acid production. In addition, the average maximum beta-glucosidase and carboxymethylcellulase productions from AT 11-2-3, AT 11-2-10, and AT 11-2-14 were about 16- and 4-folds higher than those of A. niger, respectively.

show abstract

Section: -Deoxy-d-glucose-resistant Propertiessupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polyploid Formation Between Aspergillus niger and Trichoderma viride For Enhanced Citric Acid Production From Cellulose

Watanapokasin

Sawasjirakij

Usami

et al. 2007

Appl Biochem Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…In conjunction with chemical mutagenesis, both procedures involved the use of the fungal growth inhibitors, nystatin (polyene antibiotic) and 2-deoxyglucose (glucose analog), that are thought to affect membrane permeability or cell wall synthesis [13,14]. In prior studies [15,16], it was shown that a small fraction of fungal mutants isolated by their resistance to the above growth inhibitors also exhibited enhanced antibiotic production. Since it has been proposed that pullulan is synthesized by A. pullulans in close proximity to its membrane [17], it was thought that the presence of either inhibitor in a solid medium might help in the selection of mutants with enhanced polysaccharide production.…”

Section: Resultsmentioning

confidence: 99%

Elevated polysaccharide production by mutants of the fungus Aureobasidium pullulans

West

Reed-Hamer

1994

FEMS Microbiology Letters

View full text Add to dashboard Cite

Two mutants of the fungus Aureobasidium pullulans ATCC 42023 were isolated that exhibited elevated polysaccharide production. Both mutants were isolated using a combination of chemical mutagenesis and resistance to growth inhibitors. It was found that both mutants elaborated higher polysaccharide levels after 7 days of growth on corn syrup or sucrose, respectively, compared to ATCC 42023. The dry weights of the mutant cells were found not to differ greatly from those of the parent cells whether corn syrup or sucrose served as the carbon source. The pullulan content of the polysaccharide synthesized by the mutants or parent cells on sucrose was consistently lower than polysaccharide synthesized on corn syrup. Using corn syrup as a carbon source, the pullulan content of the polysaccharide elaborated by the parent was higher than either mutant. The inverse was found to occur with respect to pullulan content when the strains were grown on sucrose as a carbon source.

show abstract

“…Of the total amount of citric acid produced annually, about 70% is utilised by the food industry because of its pleasant acid taste, high solubility in water, chelating, antioxidant and buffering properties. About 12% is utilised by pharmaceutical industries as liquid elixirs, flavouring, anticoagulant and preservative while the remaining 18% is utilised by other industries such as cosmetics, toiletry, detergent, textile, oil recovery, paper etc (Archer, 2000;Hang & Woodams, 1985;Pandey et al, 2001;Sarangbin, Kirimura & Usami, 1993;Soccol, Prado, Vandenberghe & Pandey, 2003). Domínguez, 2010;Nadeem et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Modelling and Simulation of Citric Acid Production from Corn Starch Hydrolysate Using Aspergillus Niger

Amenaghawon¹,

Aisien²

2012

ENRR

View full text Add to dashboard Cite

The kinetics of citric acid fermentation from corn starch hydrolysate using Aspergillus niger ATCC 9142 was studied in a batch fermenter. A general model for citric acid production was formulated. Four kinetic models, Monod, Haldane, logistic and hyperbolic for describing the growth of the fermenting microorganism were explored. The validity of the models in terms of predicting growth of the fermenting organism was determined by fitting each kinetic model to experimental data collected in the course of this work. Comparison of experimental results to model predicted results showed that only the hyperbolic model was able to accurately replicate the experimental results. This was evident from the high level of correlation between the experimental and model predicted results. The kinetic parameters for cell growth, substrate consumption and product formation µ max , Y x/s , Y p/x , K s and K p as calculated by the hyperbolic model are 0.01320h -1 , 0.711g/g, 13.6708g/g, 0.0006g/dm 3 , and 0.2572 g/dm 3 respectively. The validated model was implemented in an advanced equation oriented modelling software to determine the effect of key process parameters on the production of citric acid.Results of simulating the model show that the production of citric acid is a growth associated process. Optimum pH, initial sugar concentration and temperature of for citric acid production were 5.5, 40w/v and 30 o C respectively.

show abstract

Citric acid production from cellobiose by 2-deoxyglucose-resistant mutant strains of Aspergillus niger in semi-solid culture

Cited by 14 publications

References 12 publications

Polyploid Formation Between Aspergillus niger and Trichoderma viride For Enhanced Citric Acid Production From Cellulose

Polyploid Formation Between Aspergillus niger and Trichoderma viride For Enhanced Citric Acid Production From Cellulose

Elevated polysaccharide production by mutants of the fungus Aureobasidium pullulans

Modelling and Simulation of Citric Acid Production from Corn Starch Hydrolysate Using Aspergillus Niger

Contact Info

Product

Resources

About