Modelling and Simulation of Citric Acid Production from Corn Starch Hydrolysate Using Aspergillus Niger

Amenaghawon, Andrew N.; Aisien, Felix Aibuedefe

doi:10.5539/enrr.v2n1p73

Cited by 11 publications

(14 citation statements)

References 26 publications

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“…However, results showed that the maximum concentration can be obtained with 14--22% of sugar. A study by Xu et al (1989) using sucrose, glucose, fructose, mannose and maltose obtained the maximum yield at a sugar concentration of 10% (w/v), except for glucose, where a 7.5% (w/v) maximum yield was obtained (Amenaghawon & Aisien 2012).…”

Section: Factors Affecting Citric Acid Fermentationmentioning

confidence: 99%

“…This technique has been proven to be economical for clear fermentation media. However, it is 50% more expensive than other recovery processes such as precipitation (Amenaghawon & Aisien 2012). In laboratory analyses, the determination and quantification of citric acid are done using spectrophotometry (Themelis & Tzanavaras 2001;Jham et al 2002), gas chromatography (Jham et al 2002), high-performance liquid chromatography (Jham et al 2002), high-resolution nuclear magnetic resonance spectroscopy (del Campo et al 2006) and the traditional method of titration (Williams 1984).…”

Section: Recovery Of Citric Acidmentioning

confidence: 99%

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Overview of citric acid production from Aspergillus niger

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Oladele

Siew

et al. 2015

Frontiers in Life Science

200

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Citric acid has high economic potential owing to its numerous applications. It is mostly produced by microbial fermentation using Aspergillus niger. In view of surges in demand and growing markets, there is always a need for the discovery and development of better production techniques and solutions to improve production yields and the efficiency of product recovery. To support the enormous scale of production, it is necessary and important for the production process to be environmentally friendly by utilizing readily available and inexpensive agro-industrial waste products, while maintaining high production yields. This article reviews the biochemistry of citric acid formation, choices of citric-acid producing microorganisms and raw materials, fermentation strategies, the effects of various fermentation conditions, citric acid recovery options and the numerous applications of citric acid, based on information drawn from the literature over the past 10 years.

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Section: Factors Affecting Citric Acid Fermentationmentioning

confidence: 99%

Section: Recovery Of Citric Acidmentioning

confidence: 99%

Overview of citric acid production from Aspergillus niger

Show

Oladele

Siew

et al. 2015

Frontiers in Life Science

200

120

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“…Pertumbuhan A. niger dihambat oleh produk yang dihasilkannya [2], [3] sehingga adalah: (3) Persamaan (3) di substitusi ke persamaan (1), sehingga diperoleh persamaan akhir untuk menghitung laju pertumbuhan yang dinyatakan pada persamaan (4). (4) Persamaan (4) …”

Section: Pendahuluanunclassified

“…[4] Woin arosc hy et. al., (2010 ) [2] Amen agha won and Aisie n (2012 ) [3] Znad et. al., K s merupakan konstanta kejenuhan Monod.…”

Section: Hasil Dan Pembahasanunclassified

“…Besarnya nilai K s pada saat konsentrasi awal glukosa 30, 50, dan 70 g/l adalah 157,5 g/l. Nilai K s hasil dari berbagai penelitian adalah 115,3839 g/l [2] dengan konsentrasi awal glukosa 150 g/l; 0,0006 g/l [3] dengan konsentrasi awal glukosa 3,032 g/l; 130,902 g/l [5] dengan konsentrasi awal glukosa 150 g/l. Nilai Ks dipengaruhi oleh jenis substrat dan mikroorganisme.…”

Section: Hasil Dan Pembahasanunclassified

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Studi Kinetika Konsumsi Glukosa oleh Aspergillus Niger dalam Produksi Bioethanol dari Lignoselulosa

Widiyanti¹

2017

Intek

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-Global warming resulted from CO 2 level increase in the atmosphere has caused elevation of earth temperature and uncertain climate changes. To prevent the rise of CO 2 in the atmosphere can be done by using biomass fuel such as bioethanol. The raw materials of bioethanol can be derived from oil palm empty fruit bunch. Enzymatic hydrolysis utilizes cellulase-producing fungus and in this research, Aspergillus niger was chosen. The glucose produced is consumed by A niger as carbon source and this is undesirable, therefore it should be minimized as low as possible. Knowing the rate of glucose consumption is important to have a model of the hydrolysis reaction rate which will be helpful in the design process on an industrial scale hydrolysis reactor.This study aimed to determine the equations that can be used to approximate the growth rate of A. niger, glucose consumption, the formation of citric acid, and the kinetic parameters used to modeling the kinetics of glucose consumption by A. niger. Kinetics of glucose consumption by A. niger was studied in batch system with variation of initial glucose concentration of 30, 50, 70 g/l. The growth rate of A. niger, glucose consumption, and the formation of citric acid were modeled using 3 equations; i.e. Monod with non-competitive product inhibition, Luedeking-Piret, and Luedeking-Piret growth associated product formation, respectively. The values of kinetic parameters such as μ max , K s , K p , were 0.65 hour -1 , 157.5 g/l, 0. Keywords-glucose, A. niger, citric acid, Monod noncompetitive product inhibition, Luedeking-Piret AbstrakPemanasan global mengakibatkan peningkatan suhu bumi dan perubahan iklim tidak menentu. Hal ini diakibatkan dari semakin bertambahnya CO 2 di atmosfer. Untuk meminimalisasi penambahan jumlah CO 2 diatmosfer dapat dilakukan dengan penggunaan bahan bakar yang berbahan baku biomassa yaitu bioetanol. Bahan baku bioetanol dapat berasal dari tandan kosong kelapa sawit (TKKS). Proses hidrolisis secara enzimatis dapat menggunakan jamur penghasil selulase seperti Aspergillus niger. Jamur A. niger menghidrolisis selulosa untuk mendapatkan glukosa. Sebagian glukosa hasil hidrolisis dikonsumsi A. niger untuk memenuhi kebutuhan sumber karbonnya. Konsumsi glukosa oleh A. niger merupakan hal yang tidak diinginkan dan sebisa mungkin diminimalkan. Besarnya laju konsumsi glukosa penting diketahui untuk mendapatkan model laju reaksi hidrolisis yang nantinya akan bermanfaat pada proses perancangan reaktor hidrolisis pada skala industri.Penelitian ini bertujuan untuk menentukan persamaan yang dapat digunakan untuk mendekati kecepatan pertumbuhan A. niger, kecepatan konsumsi glukosa, kecepatan produksi asam sitrat oleh A. niger, serta mengetahui parameter-parameter kinetika yang digunakan dalam penyusunan model kinetika konsumsi glukosa oleh A. niger. Kinetika konsumsi glukosa oleh A. niger dipelajari secara batch dengan variasi konsentrasi awal glukosa 30, 50, 70 g/l. Laju pertumbuhan, konsumsi glukosa, produksi asam sitrat oleh A. niger didekati dengan pe...

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Costly effective bioleaching of valuable metals from low grade ore using Aspergillus nidulans

Ahmed,

Mohammed,

Kawady

et al. 2023

Int. J. Environ. Sci. Technol.

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This research investigated the feasibility of employing organic acids, like citric acid, produced by Aspergillus nidulans MT355567 in a bioleaching process to recover uranium (U) from a low-grade rock sample. The optimal conditions for fungal growth and maximum citric acid (CA) synthesis across three distinct media were determined. The maximum citric acid concentration was produced on medium made from wheat bran (83%) and tea waste (77%). An investigation was carried out to see how citric acid and, by consequence, uranium bioleaching affinity, were affected by varying carbon sources, nitrogen sources, pH, temperature, incubation period, ore particle size, and the solid–liquid ratio. At 25 °C and a pH of 5.0, media containing 100 g/L of sucrose as a carbon source and peptone as a nitrogen source made the highest yield of citric acid and U bioleaching. Higher U bioleaching was achieved with ore particles 0.075 mm at a ratio of 2 g/L after only 30 min of contact with the fungal filtrate. Iron interference has a negative impact on uranium extraction. Interestingly, none of the conditions applied to enhance CA synthesis and U-bioleaching caused iron (Fe) dissolution. Based on these findings, it appears that bioleaching using A. nidulans MT355567 metabolic products is a promising economic and ecofriendly technology for extracting uranium from low-grade ore that might be adopted on a pilot scale. Graphical abstract summarizing the experimental workflow for bioleaching of uranium from low-grade ore using citric acid produced by Aspergillus nidulans. The process involved optimizing A. nidulans growth and citric acid biosynthesis, evaluating factors influencing bioleaching activity of the acid metabolite solutions, and finally applying the optimized conditions to bioleach uranium from the ore sample. The schematic illustrates the key steps and the optimal condition for Aspergillus nidulans growth medium preparation using agricultural wastes, downstream application of metabolite synthesized for uranium bioleaching

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Modelling and Simulation of Citric Acid Production from Corn Starch Hydrolysate Using Aspergillus Niger

Cited by 11 publications

References 26 publications

Overview of citric acid production from Aspergillus niger

Overview of citric acid production from Aspergillus niger

Studi Kinetika Konsumsi Glukosa oleh Aspergillus Niger dalam Produksi Bioethanol dari Lignoselulosa

Costly effective bioleaching of valuable metals from low grade ore using Aspergillus nidulans

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