Citrate-capped iron oxide nanoparticles impair the osteogenic differentiation potential of rat mesenchymal stem cells

Abstract

The cellular uptake of citrate-capped iron oxide nanoparticles can impair the osteogenic differentiation of MSCs.

Formation of Protein Corona on Rhodium Citrate-Functionalized Magnetic Nanoparticles and Their Interaction with Human Macrophages

“…In fact, no sedimentation nor important pH variation (pH = 6.8 ± 0.2) were observed during the 24 months of evaluation of the synthesized Mag-RhCit sample. Besides, the colloidal parameters of a two-year-old sample, HD ∼147 nm, ZP ∼-40 mV and PDI ∼0.17, indicates a condition of high colloidal stability, corroborating visual observations [26,27]. However, the stability was also monitored by means of these colloidal parameters over a long period of time, as shown in Figure 1.…”

“…Magnetite NPs (Fe 3 O 4 ) were first obtained via alkaline co-precipitation of Fe 2+ and Fe 3+ ions and subsequently oxidized to maghemite (γ-Fe 2 O 3 ) [26]. Typically, 50.0 mL of an aqueous solution containing 50 mmol of FeCl 2 ⋅4H 2 O, 25 mmol of FeCl 3 ⋅6H 2 O and 20 mmol of HCl was rapidly poured into 250.0 mL of 1.0 mol/L NH 4 OH solution under stirring (1000 rpm) at room temperature, for 30 min.…”

<p>The influence of female mice age on biodistribution and biocompatibility of citrate-coated magnetic nanoparticles</p>

“…Moreover, citrate detachment from the surface of MNPs under physiological conditions is negligible, and the physical-chemical characteristics of citrate-capped MNPs can be preserved over years. 21,27 Indeed, previous in vivo investigations have shown the biocompatibility and nonmutagenic effects of citrate coatings, [28][29][30] in spite of adverse effects described in in vitro tests. 31 However, citrate-coated MNPs can be colloidally unstable in a biological system when dispersed in a medium with high ionic strength, such as PBS buffer.…”

“…Maghemite (γ-Fe 2 O 3 ) nanoparticles were obtained via the oxidation of magnetite nanoparticles synthesized by the alkaline hydrolysis of ions Fe(II) and Fe(III). 21 γ-Fe 2 O 3 nanoparticles were functionalized with citrate ions as described previously. 22 The functionalized nanoparticles were dispersed in water, yielding stable colloidal suspension at neutral pH.…”

Maghemite–gold core–shell nanostructures (γ-Fe2O3@Au) surface-functionalized with aluminium phthalocyanine for multi-task imaging and therapy

“…1 citrate-capped maghemite (g-Fe 2 O 3 ) nanoparticles were synthesized using a slightly modied procedure already described in the literature. 13 Briey, 50 mL of aqueous solution containing 50 mmol of Fe 2+ , 25 mmol of Fe 3+ and 20 mmol of HCl were quickly poured into 250 mL of NH 4 OH aqueous solution (1 mol L À1 ), under vigorous stirring (1000 rpm) at room temperature. The as-formed black precipitate of magnetite (Fe 3 O 4 ) was magnetically separated and washed with water several times until the solution reached neutral pH.…”