Cissampelos sympodialis (Menispermaceae): A Novel Phytotherapic Weapon Against Allergic Diseases?

Piuvezam, Márcia Regina; Bezerra-Santos, C.R.; Bozza, Patrı́cia T.; Bandeira‐Melo, Christianne; Vieira, Giciane Carvalho; Costa, Hermann Ferreira

doi:10.5772/25739

Cited by 3 publications

(1 citation statement)

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“…This step was followed by several extractions with chloroform and a final purification by TLC [ 12 , 36 ]. Detailed information on the purification and structure of the warifteine was previously published [ 2 , 15 ] and its structure is shown in Figure 1 . The warifteine used throughout was in its crystal form and had a purity of 100%.…”

Section: Methodsmentioning

confidence: 99%

Warifteine, an Alkaloid Purified fromCissampelos sympodialis, Inhibits Neutrophil MigrationIn VitroandIn Vivo

Lima

Rocha

Guimarães-Costa

et al. 2014

Journal of Immunology Research

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Cissampelos sympodialis Eichl is a plant from the Northeast and Southeast of Brazil. Its root infusion is popularly used for treatment of inflammatory and allergic diseases. We investigated whether warifteine, its main alkaloid, would have anti-inflammatory effect due to a blockage of neutrophil function. In vivo warifteine treatment inhibited casein-induced neutrophil migration to the peritoneal cavity but did not inhibit neutrophil mobilization from the bone marrow. Analysis of the direct effect of warifteine upon neutrophil adherence and migration in vitro demonstrated that the alkaloid decreased cell adhesion to P and E-selectin-transfected cells. In addition, fLMP-induced neutrophil migration in a transwell system was blocked by warifteine; this effect was mimicked by cAMP mimetic/inducing substances, and warifteine increased intracellular cAMP levels in neutrophils. The production of DNA extracellular traps (NETs) was also blocked by warifteine but there was no alteration on PMA-induced oxidative burst or LPS-stimulated TNFα secretion. Taken together, our data indicate that the alkaloid warifteine is a potent anti-inflammatory substance and that it has an effect on neutrophil migration through a decrease in both cell adhesion and migration.

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Section: Methodsmentioning

confidence: 99%