Cisplatin influences the skin ion transport – An in vitro study

Hołyńska-Iwan, Iga; Sobiesiak, Marta

doi:10.1016/j.biopha.2020.110502

Cited by 5 publications

(19 citation statements)

References 30 publications

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“…Among others, it is involved in temperature regulation, water management, the metabolism of vitamin D and lipids, and the perception of various stimuli, including touch and pain [ 17 , 30 , 31 ]. The proposed experimental model for assessing electrophysiological parameters in the modified Ussing chamber is based on an analysis of full-thickness skin fragments with preserved layered structure and nerve endings [ 18 , 19 , 33 ]. The Ussing chamber has been frequently used to analyze pathomechanisms of various disorders and diseases associated with the disrupted function of ion transporters and/or ion channels [ 33 , 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…The proposed experimental model for assessing electrophysiological parameters in the modified Ussing chamber is based on an analysis of full-thickness skin fragments with preserved layered structure and nerve endings [ 18 , 19 , 33 ]. The Ussing chamber has been frequently used to analyze pathomechanisms of various disorders and diseases associated with the disrupted function of ion transporters and/or ion channels [ 33 , 34 , 35 ]. The modification of the Ussing chamber involved positioning an analyzed specimen horizontally and its mechanical stimulation by fluids using a nozzle located 3–5 mm above the surface of the examined tissue sample [ 18 , 19 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…The Ussing chamber has been frequently used to analyze pathomechanisms of various disorders and diseases associated with the disrupted function of ion transporters and/or ion channels [ 33 , 34 , 35 ]. The modification of the Ussing chamber involved positioning an analyzed specimen horizontally and its mechanical stimulation by fluids using a nozzle located 3–5 mm above the surface of the examined tissue sample [ 18 , 19 , 33 ]. This modification extends the use of the Ussing chamber, enabling the study of the impact of mechanical and mechanical-chemical stimulations on the transport of ions in the tested tissue preparations.…”

Section: Discussionmentioning

confidence: 99%

“…Using the Ussing chamber to measure changes in ion transport is a valuable tool for assessing the functioning of ion pumps, channels, and co-transporters and, thus, for determining the response of the tissue to the stimuli applied [ 18 , 19 , 33 ]. The examined skin fragments were full-thickness, with preserved layering and nerve endings, which allowed the assessment of the cell’s ability to modify ion transport after the incubation with diclofenac, a mechanical stimulation with the iso-osmotic Ringer’s solution or mechanical-chemical stimulations with the blockers of sodium ion transport, namely amiloride, and/or chloride ion transport, namely bumetanide ( Table 5 ).…”

Section: Discussionmentioning

confidence: 99%

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The Impact of Diclofenac Gel on Ion Transport in the Rabbit (Oryctolagus cuniculus) Skin: An In Vitro Study

et al. 2023

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Diclofenac belongs to the non-steroidal anti-inflammatory drugs with analgesic, antipyretic and anti-inflammatory effects. Diclofenac administration on the skin may be associated with the appearance of side effects. The study aimed to evaluate the impact of diclofenac gel on transepithelial electrophysiological parameters of the 55 rabbit abdomen skin specimens. The electric parameters were analyzed in a modified Ussing chamber. The resistance (R) of the skin specimens treated with diclofenac gel significantly increased, which could be related to the reduction in the water content in intercellular spaces and, consequently, tighter adhesion of the cells. Increased electric potential (PD) was also observed in the skin specimens treated with diclofenac gel. The increase in both R and PD measured under stationary conditions was most likely caused by a transient and reversible increase in sodium ion transport, as the R and PD values decreased after the diclofenac gel was washed away. However, diclofenac gel did not affect the maximum and minimum PDs measured during stimulations. Therefore, it seems that diclofenac gel does not affect the perception of stimuli in the model system used.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The Impact of Diclofenac Gel on Ion Transport in the Rabbit (Oryctolagus cuniculus) Skin: An In Vitro Study

et al. 2023

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“…Since the patient had a known history of ovarian carcinosarcoma and had been initially treated with paclitaxel and carboplatin, and then switched to docetaxel/cisplatin/bevacizumab, chemotherapy-related drug reaction was first considered as the possible cause. In fact, it has been reported that dermatological toxicities, from diffuse macules and papules to rarely life-threatening erythema multiforme, toxic epidermal necrolysis, and Stevens-Johnson syndrome, clearly represent one of the major adverse events associated with these agents [12][13][14]. In addition, inner thigh is an anatomic site which is easily irritated by friction, allowing bacteria and other germs to grow and causing skin rash.…”

Section: Discussionmentioning

confidence: 99%

Cutaneous metastasis of carcinomatous component of ovarian carcinosarcoma: A case report and review of the literature

et al. 2022

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Skin metastasis of ovarian cancer is extremely rare. We report an unusual case of ovarian carcinosarcoma with cutaneous metastasis of carcinomatous component that displayed distinct clinical manifestation. A 48-year-old woman presented to the dermatologist complaining of a new onset of erythematous, plaque-like skin rash with multiple small nodules on the left inner thigh, the area measuring 8 × 5cm. While the patient had no history of dermatologic conditions, she underwent a total hysterectomy and bilateral salpingo-oophorectomy, omentectomy, and lymph node dissection 16 months ago with a pathology confirmed stage IIIC ovarian carcinosarcoma. Of note, the carcinomatous component, mainly adenocarcinoma with hybrid features of seromucinous, endometrioid and minor high-grade serous carcinoma, involved bilateral fallopian tubes, omentum, and parametrium with extensive lymph node metastases. A skin biopsy specimen revealed an adenocarcinoma involving epidermis, dermis, and subcutaneous tissue with nodular contours, consistent with metastatic carcinomatous component of carcinosarcoma. Both carcinomatous component of primary ovarian carcinosarcoma and metastatic adenocarcinoma in the skin demonstrated Pax8, WT-1, and ER positivity and a mutation pattern of p53. The patient passed away 15 months after identification of skin metastasis. This case represents a unique example of cutaneous metastasis of ovarian carcinosarcoma with distinct clinical manifestation and detailed histopathological description. Alertness to the possibility of cutaneous metastasis, in combination with clinical history, morphological and immunohistochemical findings, is critical for a definitive classification.

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Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin

Hołyńska-Iwan

Sobiesiak

Kowalczyk³

et al. 2023

Sci Rep

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Measurements of transepithelial potential and resistance in tissue and organ model systems enable the evaluation of the Ni2+ effect on the epithelial sodium channels, aquaporin 3, and the sodium–potassium pump in the epithelial cells. The aim of the presented study was to assess the immediate and prolonged effect of nickel ions on the transport of sodium ions in tissues exposed to direct contact with nickel, including airways, digestive tract and the skin. The influence of 0.1 mM nickel solution was performed on the trachea (n = 34), intestine (n = 44), and skin (n = 51) samples descended from 16 New Zealand albino rabbits. The electrophysiological parameters were measured in a modified Ussing chamber in stationary conditions and during a 15-s mechanical-chemical stimulation. A statistically significant decrease in the electric resistance values and the smallest range of the measured potential were observed for the Ni-treated trachea specimens. The use of nickel solution did not affect the sodium transport in the intestine epithelium. The skin fragments showed altered sodium ion transport, as demonstrated by the lower range and intensity of the measured potential. The gastrointestinal tract seems to be an organ best adapted to contact with nickel ions. In airways, nickel ions most likely enter epithelial cells and the space between them, modifying proteins and the airway surface liquid. The skin turned out to be the most sensitive tissue to the intensification of sodium ion transport through nickel ions.

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Cisplatin influences the skin ion transport – An in vitro study

Cited by 5 publications

References 30 publications

The Impact of Diclofenac Gel on Ion Transport in the Rabbit (Oryctolagus cuniculus) Skin: An In Vitro Study

The Impact of Diclofenac Gel on Ion Transport in the Rabbit (Oryctolagus cuniculus) Skin: An In Vitro Study

Cutaneous metastasis of carcinomatous component of ovarian carcinosarcoma: A case report and review of the literature

Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin

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