Cisplatin (CDDP) triggers cell death of MCF-7 cells following disruption of intracellular calcium ([Ca&lt;sup&gt;2+&lt;/sup&gt;]&lt;sub&gt;i&lt;/sub&gt;) homeostasis

Al-Taweel, Nawaf; Varghese, Elizabeth; Florea, Ana; Büsselberg, Dietrich

doi:10.2131/jts.39.765

Cited by 42 publications

(28 citation statements)

References 28 publications

(38 reference statements)

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“…The MTS colorimetric assay was used to determine cytotoxic effects of CLO extract at final concentrations of 50–1000 μg/ml. After 72 hrs of incubation, 10 μl of MTS (Promega, Madison, WI, USA) was added to each well according to the CellTiter 96 ® AQueous One Solution Cell Proliferation Assay protocol.…”

Section: Methodsmentioning

confidence: 99%

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Kubatka

Uramova

Kello

et al. 2017

J Cellular Molecular Medi

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It is supposed that plant functional foods, rich in phytochemicals, may potentially have preventive effects in carcinogenesis. In this study, the anticancer effects of cloves in the in vivo and in vitro mammary carcinoma model were assessed. Dried flower buds of cloves (CLOs) were used at two concentrations of 0.1% and 1% through diet during 13 weeks after the application of chemocarcinogen. After autopsy, histopathological and immunohistochemical analyses of rat mammary carcinomas were performed. Moreover, in vitro evaluation using MCF‐7 cells was carried out. Dietary administered CLO caused the dose‐dependent decrease in tumour frequency by 47.5% and 58.5% when compared to control. Analysis of carcinoma cells in animals showed bcl‐2, Ki67, VEGFA, CD24 and CD44 expression decrease and Bax, caspase‐3 and ALDH1 expression increase after high‐dose CLO administration. MDA levels were substantially decreased in rat carcinomas in both CLO groups. The evaluation of histone modifications revealed increase in lysine trimethylations and acetylations (H4K20me3, H4K16ac) in carcinomas after CLO administration. TIMP3 promoter methylation levels of CpG3, CpG4, CpG5 islands were altered in treated cancer cells. An increase in total RASSF1A promoter methylation (three CpG sites) in CLO 1 group was found. In vitro studies showed antiproliferative and pro‐apoptotic effects of CLO extract in MCF‐7 cells (analyses of cytotoxicity, Brdu, cell cycle, annexin V/PI, caspase‐7, Bcl‐2 and mitochondrial membrane potential). This study showed a significant anticancer effect of clove buds in the mammary carcinoma model in vivo and in vitro.

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Section: Methodsmentioning

confidence: 99%

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Kubatka

Uramova

Kello

et al. 2017

J Cellular Molecular Medi

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“…Cancer cells might become resistant to these Pt (II) drugs due to detoxification, decreased cellular uptake, DNA repair, increased drug efflux and diminished apoptosis. Platinum (IV) prodrugs are considered one of the approaches developed to overcome the drawbacks of the Pt (II) drugs . The octahedral Pt (IV) complexes are activated by reduction inside the cell to the active platinum (II) counterparts before binding to their target DNA (Figure ) .…”

Section: Introductionmentioning

confidence: 99%

“…Platinum (IV) prodrugs are considered one of the approaches developed to overcome the drawbacks of the Pt (II) drugs. [14][15][16][17][18] The octahedral Pt (IV) complexes are activated by reduction inside the cell to the active platinum (II) counterparts before binding to their target DNA ( Figure 2). [19][20][21][22] The axial ligands in Pt (IV) complexes that are released upon reduction can be used to control important properties of the drug, such as stability, reduction potential and lipophilicity.…”

Section: Introductionmentioning

confidence: 99%

Anti‐tumor platinum (IV) complexes bearing the anti‐inflammatory drug naproxen in the axial position

Tolan

Abdel‐Monem

El-Nagar

2019

Applied Organom Chemis

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The role of inflammation in cancer generation is gaining importance in the field of cancer research. The chemo‐anti‐inflammatory strategy that involves using non‐steroidal anti‐inflammatory drug compounds as effective anti‐tumor agents is being acceded globally. In the present study, seven new Pt (IV) complexes based on cisplatin, carboplatin and oxaliplatin scaffold bearing the anti‐inflammatory drug naproxen in the axial position were synthesized and characterized by elemental analysis, ESI‐MS, Fourier transform‐infrared, 1H‐ and 195Pt‐NMR spectroscopy. The reduction behavior in the presence of ascorbic acid was studied using high‐performance liquid chromatography. The cytotoxicity against two human breast cell lines and the anti‐inflammatory properties were evaluated. All the complexes are able to promote a comparable activity, with average three‐ and 13‐fold more cytotoxic than cisplatin against MCF7 and MDA‐MB‐231 cell lines, respectively. The complexes show remarkable anti‐inflammatory effects, which indicated their potential in treating cancer associated with inflammation and reducing side‐effects of chemotherapy.

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“…Although molecular-targeted cancer therapy has advanced considerably, chemotherapy is the primary treatment for lung cancer cases that are unresectable. For patients with advanced NSCLC, the platinum-based two-drug combination regimen is a standard chemotherapy (3,4). Cisplatin (CDDP)-based chemotherapy has improved the survival rate of patients with NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Effect of the p53α gene on the chemosensitivity of the H1299 human lung adenocarcinoma cell line

2017

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To investigate the effects of tumor protein p53 (p53 or TP53) α gene on the chemosensitivity of the H1299 human lung adenocarcinoma cell line, the recombinant vector pEGFP-p53α was constructed. The vector pEGFP-p53α was transfected into the cultured p53-null H1299 cells using Lipofectamine 2000. The G418-resistant cells were then selected. The expression of the p53α gene in these cells was examined using reverse transcription-polymerase chain reaction, and TP53 protein expression was examined using western blot analysis and immunocytochemistry. An MTT assay and colony formation assay were used to analyze the response of the transfected cells to cisplatin (CDDP). DAPI staining was used to determine the level of apoptosis of the transfected cells. The transfected H1299 human lung adenocarcinoma cells stably expressed TP53 protein. The MTT assay demonstrated that the 50% inhibitory concentrations for the H1299, H1299/pEGFP-N1 and H1299/pEGFP-p53α cells were 28, 24 and 18 µmol/l, respectively. The survival rate of H1299/pEGFP-p53α cells was significantly reduced compared with that of H1299 and H1299/pEGFP-N1 cells (P<0.05). The colony formation assay and DAPI staining identified that the colony formation rate and the number of apoptotic cells of H1299/pEGFP-p53α were significantly reduced, compared with those of the H1299 and H1299/pEGFP-N1 cells (P<0.05). Therefor, the present study demonstrated that the transfection of H1299 cells with the p53α gene resulted in an increase in sensitivity to CDDP chemotherapy. The combination of CDDP and gene therapy for H1299 lung adenocarcinoma cell line provides an experimental basis for clinical research.

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Cisplatin (CDDP) triggers cell death of MCF-7 cells following disruption of intracellular calcium ([Ca<sup>2+</sup>]<sub>i</sub>) homeostasis

Cited by 42 publications

References 28 publications

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Anti‐tumor platinum (IV) complexes bearing the anti‐inflammatory drug naproxen in the axial position

Effect of the p53α gene on the chemosensitivity of the H1299 human lung adenocarcinoma cell line

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